Hostname: page-component-848d4c4894-sjtt6 Total loading time: 0 Render date: 2024-06-16T07:53:34.926Z Has data issue: false hasContentIssue false
  • English
  • Français

Alpha 1 Integrin and Collagen Type III in C2C12 Cell Lines

Published online by Cambridge University Press:  02 July 2020

C. DiLullo ,
J. Malsbury  and
P. M. Mattioli
C. DiLullo
Affiliation:
Department of Anatomy, Philadelphia College of Osteopathic Medicine, Philadelphia, PA19131.
J. Malsbury
Affiliation:
Department of Anatomy, Philadelphia College of Osteopathic Medicine, Philadelphia, PA19131.
P. M. Mattioli
Affiliation:
Department of Anatomy, Philadelphia College of Osteopathic Medicine, Philadelphia, PA19131.
Get access

Extract

In nascent cultured primary chick skeletal myocytes, a l integrin is observed to have a punctate distribution. As early as day 3 in culture, however, it can be found to reorganize into periodic doublet bands. Subsequent to a l integrin reorganization, collagen type III has been found to colocalize with these periodic α l integrin bands. The coordinated appearance of these two proteins is suggestive of a possible regulatory role for one or both proteins in muscle development.

To ascertain whether this same αl integrin/collagen type III reorganization occurs in muscle cell lines, differentiating myocytes from the C2C12 cell line were examined for the temporal appearance and localization of both proteins. Cultured C2C12 cells were immunofluorescently labeled with antibodies to al integrin, collagen type III and other muscle specific proteins on days 1, 3, 6, 9 and 11 after growth medium was replaced with differentiation medium.

Type
Developmental/Reproductive Biology
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 962 - 963
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

References:

1)DiLullo, C. et al, Basic Appl. Myol., 8(4) (1998) 325–340.Google Scholar
2)Belkin, A.M. et al., J. Cell Biol, 132 (1996) 211–226.CrossRefGoogle Scholar
3)Gotwals, P.J. et al., J. Clin. Invest., 97(11) (1996) 2469–2477.CrossRefGoogle Scholar
4)Yamada, K.M. and Miyamato, S., Cur. Opinion Cell Biol, 7 (1995) 681–689.CrossRefGoogle Scholar