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Histopathological Effects on Gills of Nile Tilapia (Oreochromis niloticus, Linnaeus, 1758) Exposed to Pb and Carbon Nanotubes

Published online by Cambridge University Press:  21 December 2016

Edison Barbieri ,
Janaína Campos-Garcia ,
Diego S. T. Martinez ,
José Roberto M. C. da Silva ,
Oswaldo Luiz Alves  and
Karina F. O. Rezende
Edison Barbieri*
Affiliation:
Instituto de Pesca, APTA- Secretaria da Agricultura e Abastecimento do Governo do Estado de São Paulo; Avenida Professor Wladimir Besnard s/no - Caixa Postal 157, 11990-000 - Cananéia, São Paulo, Brazil
Janaína Campos-Garcia
Affiliation:
Instituto de Pesca, APTA- Secretaria da Agricultura e Abastecimento do Governo do Estado de São Paulo; Avenida Professor Wladimir Besnard s/no - Caixa Postal 157, 11990-000 - Cananéia, São Paulo, Brazil
Diego S. T. Martinez
Affiliation:
Brazilian Nanotechnology National Laboratory (LNNano), Brazilian Center for Research in Energy and Materials (CNPEM), Rua Giuseppe Máximo Scolfaro, 10.000 Polo II de Alta Tecnologia de Campinas - CEP 13083-970, Campinas, São Paulo, Brasil
José Roberto M. C. da Silva
Affiliation:
Instituto de Ciências Biomédicas, Universidade de São Paulo. Av. Prof. Lineu Prestes, 1524 - CEP 05508-900 - São Paulo, São Paulo, Brasil
Oswaldo Luiz Alves
Affiliation:
Solid State Chemistry Laboratory and NanoBioss Laboratory, Institute of Chemistry, University of Campinas, Rua Josué de Castro, 126 - Caixa Postal 6154, 13083-970 - Campinas, São Paulo, Brasil
Karina F. O. Rezende
Affiliation:
Instituto de Ciências Biomédicas, Universidade de São Paulo. Av. Prof. Lineu Prestes, 1524 - CEP 05508-900 - São Paulo, São Paulo, Brasil
*
*Corresponding author.edisonbarbieri@yahoo.com.br
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Abstract

The effect of heavy metal in fish has been the focus of extensive research for many years. However, the combined effect of heavy metals and nanomaterials is still a new subject that needs to be studied. The aim of this study was to examine histopathologic alterations in the gills of Nile tilapia (Oreochromis niloticus) to determine possible effects of lead (Pb), carbon nanotubes, and Pb+carbon nanotubes on their histological integrity, and if this biological system can be used as a tool for evaluating water quality in monitoring programs. For this, tilapia were exposed to Pb, carbon nanotubes and Pb+carbon nanotubes for 4 days. The main alterations observed were epithelial structure, hyperplasia and displacement of epithelial cells, and alterations of the structure and occurrence of aneurysms in the secondary lamella. The most severe alterations were related to the Pb+carbon nanotubes. We conclude that the oxidized multi-walled carbon nanotubes enhanced the acute lead toxicity in Nile tilapias. This work draws attention to the implications of carbon nanomaterials released in the aquatic environment and their interaction with classical pollutants.

Keywords

carbon nanotubenanoparticlesfishhistology
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 22 , Issue 6 , December 2016 , pp. 1162 - 1169
Copyright
© Microscopy Society of America 2016 

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