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Development and characterization of tetraploid castor plants

Published online by Cambridge University Press:  31 January 2020

K. Baghyalakshmi ,
Mobeen Shaik ,
Manmode Darpan Mohanrao ,
Ranjan Kumar Shaw ,
C. Lavanya ,
T. Manjunatha  and
S. Senthilvel Open the ORCID record for S. Senthilvel [Opens in a new window]
K. Baghyalakshmi
Affiliation:
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad – 500030, India ICAR-Central Institute for Cotton Research, Regional Station, Coimbatore – 641003, India
Mobeen Shaik
Affiliation:
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad – 500030, India
Manmode Darpan Mohanrao
Affiliation:
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad – 500030, India Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad – 500030, India
Ranjan Kumar Shaw
Affiliation:
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad – 500030, India
C. Lavanya
Affiliation:
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad – 500030, India
T. Manjunatha
Affiliation:
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad – 500030, India
S. Senthilvel*
Affiliation:
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad – 500030, India
*
*Corresponding author. E-mail: senthilvel.senapathy@icar.gov.in
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Abstract

Castor is a prime industrial crop belonging to a monotypic genus and its genetic improvement depends on creating desired variability in the primary gene pool. This study reports the development of tetraploid castor plants through colchicine treatment. Seeds of three castor genotypes were soaked in aqueous solutions of colchicine with variable concentrations, and the LD50 value was determined. Of 1010 treated field-raised plants, three were identified as potential polyploids based on increases in a guard cell size and reductions in the number of stomata. The putative polyploid plants were selfed and the progeny were subjected to meiotic analysis. All the progeny were found to be tetraploid. The pairing of chromosomes was abnormal with univalent to octavalent configurations during meiosis-I, but the later parts of meiosis were normal. Seasonal variations in pollen fertility indicated the possible role of temperature-sensitive male sterility in causing the sterility in tetraploid plants. The tetraploid plants were phenotypically comparable with their diploid counterparts, but produced substantially bigger seeds. Thus, these tetraploid plants are valuable resources for basic and applied research in castor.

Keywords

autopolyploidcolchicinemeiotic behaviourpollen sterilityRicinus communis L.
Type
Research Article
Information
Plant Genetic Resources , Volume 18 , Issue 2 , April 2020 , pp. 98 - 104
Copyright
Copyright © NIAB 2020

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