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Screening for pathotype-specific resistance to broad bean wilt virus 2 and cucumber mosaic virus in pepper (Capsicum annuum L.)

Published online by Cambridge University Press:  17 September 2021

Kyeong-Jae Heo ,
Boram Choi ,
Myung-Hwi Kim ,
Min-Jun Kwon ,
Young-Eun Cho ,
Sun-Jung Kwon  and
Jang-Kyun Seo Open the ORCID record for Jang-Kyun Seo [Opens in a new window]
Kyeong-Jae Heo
Affiliation:
Department of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Republic of Korea
Boram Choi
Affiliation:
Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea
Myung-Hwi Kim
Affiliation:
Department of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Republic of Korea Integrated Major in Global Smart Farm, Seoul National University, Seoul 08826, Republic of Korea
Min-Jun Kwon
Affiliation:
Department of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Republic of Korea
Young-Eun Cho
Affiliation:
Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea
Sun-Jung Kwon
Affiliation:
Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea
Jang-Kyun Seo*
Affiliation:
Department of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Republic of Korea Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea Integrated Major in Global Smart Farm, Seoul National University, Seoul 08826, Republic of Korea
*
Author for correspondence: Jang-Kyun Seo, E-mail: jangseo@snu.ac.kr
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Abstract

Two aphid-transmitted RNA viruses, broad bean wilt virus 2 (BBWV2) and cucumber mosaic virus (CMV), are the most prevalent viruses in Korean pepper fields and cause chronic damage in pepper production. In this study, we employed a screening system for pathotype-specific resistance of pepper germplasm to BBWV2 and CMV by utilizing infectious cDNA clones of different pathotypes of the viruses (two BBWV2 strains and three CMV strains). We first examined pathogenic characteristics of the BBWV2 and CMV strains in various plant species and their phylogenetic positions in the virus population structures. We then screened 34 commercial pepper cultivars and seven accessions for resistance. While 21 pepper cultivars were resistant to CMV Fny strain, only two cultivars were resistant to CMV P1 strain. We also found only one cultivar partially resistant to BBWV2 RP1 strain. However, all tested commercial pepper cultivars were susceptible to the resistance-breaking CMV strain GTN (CMV-GTN) and BBWV2 severe strain PAP1 (BBWV2-PAP1), suggesting that breeding new cultivars resistant to these virus strains is necessary. Fortunately, we identified several pepper accessions that were resistant or partially resistant to CMV-GTN and one symptomless accession despite systemic infection with BBWV2-PAP1. These genetic resources will be useful in pepper breeding programs to deploy resistance to BBWV2 and CMV.

Keywords

Broad bean wilt virus 2cucumber mosaic viruspepperresistance screening
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 5 , October 2021 , pp. 405 - 410
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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Footnotes

*

These authors contributed equally to the article.

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