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Chemical Synthesis of Shiga Toxin Subunit B Using a Next-Generation Traceless “Helping Hand” Solubilizing Tag

23 September 2019, Version 2
Working Paper
This content is an early or alternative research output and has not been peer-reviewed by Cambridge University Press at the time of posting.

Abstract

This manuscript describes the development and characterization of a second-generation "helping hand" solubilizing tag. This semipermanent tag aids solubility of peptides during purification and handling steps and is compatible with common native chemical ligation conditions. The utility of this tag is demonstrated by the synthesis of the Shiga toxin B subunit.

Keywords

Native Chemical Ligation
Peptide synthesis
solubilizing groups
traceless tag
helping hand
Shiga toxin (Stx)
chemical Protein Synthesis

Supplementary materials

Title
Description
Actions
Title
HH2-Shiga ESI FINAL
Description
Actions
Title
HH2-main figures FINAL
Description
Actions

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Now Published

Chemical synthesis of Shiga toxin subunit B using a next-generation traceless “helping hand” solubilizing tag [opens in a new tab]
James M. Fulcher, Mark E. Petersen, Riley J. Giesler, Zachary S. Cruz, Debra M. Eckert, J. Nicholas Francis, Eric M. Kawamoto, Michael T. Jacobsen, Michael S. Kay journal article
Organic & Biomolecular Chemistry , Volume 17, Issue 48
Online publication date: 2019

Version History

Sep 23, 2019 Version 2

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv.9759314.v2
D O I: 10.26434/chemrxiv.9759314.v2 [opens in a new tab]

Funding

NIH AI150464
NIH AI120414
DoD W81XWH1810116

Author’s competing interest statement

MSK and DME are equity holders in Navigen Inc, which is developing D-peptide inhibitors.

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