Bovine Serum Albumin as A Superior “FP-Tag” for High-Throughput Glycosyltransferase Assay

Here, we report the development of a high-throughput “FP-Tag” OGT assay with bovine serum albumin as a low-cost and superior “FP-Tag”. With this assay, 2-methyleurotinone was identified as a low M-range OGT inhibitor. This type of assay with BSA as “FP-Tag” would find more applications with other glycosyltransferases.