PNA Microprobe for Label-Free Detection of Nucleic Acid Repeat Mutations

We present a PNA-based microprobe sensing platform to detect nucleic acid repeat mutations by electrochemical impedance spectroscopy. The microprobe platform discriminated Huntington’s disease-associated CAG repeats in cell-derived total RNA. This sensitive, label-free, and PCR-free detection strategy has the potential to detect a plethora of length mutation disorders.