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Supramolecular methods: the 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) transport assay

20 October 2021, Version 2
Working Paper
This content is an early or alternative research output and has not been peer-reviewed by Cambridge University Press at the time of posting.

Abstract

The vesicular anion transport activity assay, which uses 8-hydroxypyrene-1,3,6-trisulfonic acid to monitor the internal pH of the vesicles (the HPTS assay), is a widely used technique for analysing the activity of anionophore facilitated transport across a phospholipid membrane. This methods paper describes the stepwise technique to conduct this transport assay, detailing both the perks and pitfalls of using this method to determine the activity of an anionophore and the transport mechanism.

Keywords

supramolecular chemistry
anion transport
HPTS assay
vesicles

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Now Published

Supramolecular methods: the 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) transport assay [opens in a new tab]
Alexander M. Gilchrist, Patrick Wang, Israel Carreira-Barral, Daniel Alonso-Carrillo, Xin Wu, Roberto Quesada, Philip A. Gale journal article
Supramolecular Chemistry
Online publication date: Nov 17, 2021

Version History

Oct 20, 2021 Version 2

Version Notes

Revised version of the manuscript.

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The content is available under CC BY 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2021-43rq6-v2
D O I: 10.26434/chemrxiv-2021-43rq6-v2 [opens in a new tab]

Funding

Australian Research Council
DP200100453
Consejería de Educación de la Junta de Castilla y León
BU067P20
Ministerio de Ciencia e Innovación
PID2020-117610RB-I00

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) have declared ethics committee/IRB approval is not relevant to this content

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