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A Nitrile-Tagged Raman Sensor for the Ratiometric Detection of Thiols in Live Cells

16 May 2023, Version 1
Working Paper
This content is an early or alternative research output and has not been peer-reviewed by Cambridge University Press at the time of posting.

Abstract

Most Raman sensors are based on Raman tags linked to bulky aromatic molecules that affect the subcellular localization. Therefore, here, we developed a small ratiometric Raman sensor, ThioRas, to effectively detect thiols in live cells. ThioRas has a nitrile group that serves as a Raman tag for the thia-Michael reaction, and its nitrile signal shifts in the presence of an adjacent double bond. The molecular weight of ThioRas (167) was sufficiently small to allow ThioRas distribution throughout cells. ThioRas and its glutathione adduct were simultaneously detected in various subcellular locations, demonstrating its potential applicability as a Raman tag for ratiometric analysis.

Keywords

Raman
thiol
sensor
imaging

Supplementary materials

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Supplementary information
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Supplementary figures, experimental procedures, characterization data, and copies of NMR spectra (PDF)
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Version History

May 16, 2023 Version 1

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2023-0lkm0
D O I: 10.26434/chemrxiv-2023-0lkm0 [opens in a new tab]

Funding

JSPS KAKENHI
22K06495
the Tokyo Biochemical Research Foundation
Kowa Life Science Foundation.

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) have declared ethics committee/IRB approval is not relevant to this content

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