Investigation of the time effect on the nanozyme-mediated oxidation of 3, 3’-diaminobenzidine by hydrogen peroxide

Time effect on the nanozyme-mediated oxidation of 3, 3’-diaminobenzidine by hydrogen peroxide was investigated. Silver-based nanomaterials were synthesized and then used as peroxidase mimics. The time-dependent activity of the 3, 3’-diaminobenzidine oxidation catalyzed by silver-based nanomaterials was calculated by probing the color intensity of the produced brown-colored indamine polymer during the reaction at different time intervals. The time-dependent activity curve was used as an index for evaluating the time effect on the process, showed that the concentration of the brown-colored indamine polymer was increased by increasing the oxidation time and then leveled off, revealing saturation of active nodes of nanoparticles with nanozyme-substrate.