Separation of Tetrahydrocannabinol Fraction from Cannabis indica Extracts by Chromatographic Method

Tetrahydrocannabinol (∆9-THC) from Cannabis indica leaves was extracted by using solvent extraction method applying condenser apparatus. The molecular and physical attributes of ∆9-THC, or tetrahydrocannabinol, were identified. The collected oil’s content and a number of additional variables were studied. The optimum oil yield was predicted. Two samples were produced with multiple parameters. Sample (B) was made by soaking the leaves for seven days, whilst sample; (A) was made by using a condenser the instrument. The derived oil’s content and multiple kinds of other factors were studied. For characterization of both samples TLC, GC-MS, IR and HPLC techniques were applied. Various physical and chemical characteristics for Tetrahydrocannabinol (∆9- THC) oil was analyzed as different values such as acid value, specific gravity, saponification value, refractive Index by titration. Some physical tests were also performed for the detection of proteins, carbohydrates, Steroids, tennins, gums and mucilage. Both samples’ results proved wide differences. Due to elevate oxidative stability and psychoactive nature Tetrahydrocannabinol (∆9-THC) oil has many medicinal applications.