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Benchtop NMR Signal Enhancement of Metabolites in Urine Extract using SABRE

12 November 2025, Version 1
Working Paper
This content is an early or alternative research output and has not been peer-reviewed by Cambridge University Press at the time of posting.

Abstract

Metabolites in a urine extract are signal-enhanced by SABRE† hyperpolarization and detected using a benchtop NMR spectrometer. Quantification by standard addition is demonstrated for endogenic urinary nicotinamide (vitamin B3). Even higher sensitivity is achieved in an automated setup for multi-scan SABRE experiments. This hyperpolarization scheme is able to expedite biomarker detection and quantification, while maintaining low infrastructural requirements.

Keywords

SABRE
NMR
Parahydrogen
Hyperpolarization
Urine
Natural Extract
Mixture analysis
Benchtop Spectrometer
Metabolites

Supplementary materials

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Description
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ESI
Description
Experimental details on sample preparation, signal acquisition and data processing. Additional experiments for reproducibility, comparison of extract and lyophilized urine and concentration extimation of nicotinamide using nh-PHIP.
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Supplementary weblinks

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Experimental data
Description
Unedited data for spectra shown in the main text and ESI in their native format (Magritek SpinSolve and Bruker).
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Version History

Nov 12, 2025 Version 1

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The content is available under CC BY 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv-2025-6dpgs
D O I: 10.26434/chemrxiv-2025-6dpgs [opens in a new tab]

Funding

Deutsche Forschungsgemeinschaft
454252029
Deutsche Forschungsgemeinschaft
BR 4175/5-1
Deutsche Forschungsgemeinschaft
528402160
Estonian Research Competency Council
TARISTU24-TK15

Author’s competing interest statement

The author(s) have declared they have no conflict of interest with regard to this content

Ethics

The author(s) declare that they have sought and gained approval from the relevant ethics committee/IRB for this research and its publication.

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