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Bioinformatics-Guided Discovery of Citrulline 4-Hydroxylase from GE81112 Biosynthesis and Rational Engineering of Its Substrate Specificity

20 August 2019, Version 1
Working Paper
This content is an early or alternative research output and has not been peer-reviewed by Cambridge University Press at the time of posting.

Abstract

We functionally characterize a nonheme dioxygenase from GE81112 biosynthesis and identify it as a citrulline hydroxylase. A bioinformatics guided engineering was performed to alter the substrate specificity of the enzyme.

Keywords

nonheme dioxygenase
biocatalysis
enzyme engineering
hydroxylation

Supplementary materials

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Now Published

Characterization of a Citrulline 4‐Hydroxylase from Nonribosomal Peptide GE81112 Biosynthesis and Engineering of Its Substrate Specificity for the Chemoenzymatic Synthesis of Enduracididine [opens in a new tab]
Christian R. Zwick, Max B. Sosa, Hans Renata journal article
Angewandte Chemie International Edition , Volume 58, Issue 52
Online publication date: Nov 11, 2019

Version History

Aug 20, 2019 Version 1

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The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv.9684545.v1
D O I: 10.26434/chemrxiv.9684545.v1 [opens in a new tab]

Funding

National Institute of General Medical Sciences
R35GM128895
Biocatalytic C -H Functionalization Logic in the Chemoenzymatic Synthesis of Therapeutic Natural Products
https://app.dimensions.ai/details/grant/grant.7615422 [opens in a new tab]

Author’s competing interest statement

No conflict of interest

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