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Nematode extraction from banana roots by the centrifugal-flotation technique

Published online by Cambridge University Press:  17 July 2008

Jean-Louis Sarah  and
Marc Boisseau
Jean-Louis Sarah
Affiliation:
 CIRAD, blvd. La Lironde, TA A-DIR / PS3, 34398 Montpellier Cedex 5, France
Marc Boisseau
Affiliation:
 CIRAD, UPR Multiplication végétative, Station de Roujols, Petit Bourg, 97170, Guadeloupe, France ; marc.boisseau@cirad.fr
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Abstract

Introduction. The centrifugal-flotation technique allows all nematode species and life stages to be separated from root debris and residual soil particles to facilitate their observation. The principle of the method applied, key advantages, starting plant material and time required are presented. Materials and methods. Necessary laboratory materials, and details of the 19 steps required for extracting nematodes from plant tissue for counting are described. Possible troubleshooting is explained.

Keywords

France (Guadeloupe)Musa sp.pest controlmethodsextractionlaboratory equipmentplant nematodescell countingFrance (Guadeloupe)Musa sp.lutte antiravageurméthodeextractionmatériel de laboratoirenématode des plantesnumération cellulaire

Information

Type
Research Article
Information
Fruits , Volume 63 , Issue 4 , July 2008 , pp. 249 - 251
Copyright
© CIRAD, EDP Sciences, 2008

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References

Coolen W.A., d’Herde C.J., A method for the quantitative extraction of nematodes from plant tissue, Ghent State Agric. Res. Cent., Ghent, Belgium, 1972, 77 p.