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Interspecific competition between the nematode-trapping fungus, Duddingtonia flagrans, and selected microorganisms and the effect of spore concentration on the efficacy of nematode trapping

Published online by Cambridge University Press:  12 April 2024

J. Grønvold ,
J. Wolstrup ,
M. Larsen ,
A. Gillespie  and
F. Giacomazzi
J. Grønvold*
Affiliation:
Section of Zoology, Department of Ecology, Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, DK-1871, Frederiksberg, C, Denmark
J. Wolstrup
Affiliation:
Section of Microbiology, Department of Ecology, Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, DK-1871, Frederiksberg, C, Denmark
M. Larsen
Affiliation:
Danish Center for Experimental Parasitology, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, 100 Dyrlægevej, DK-1870, Frederiksberg, C, Denmark
A. Gillespie
Affiliation:
Chr. Hansen A/S, Bøge Allé 10–12, 2970, Hørsholm, Denmark
F. Giacomazzi
Affiliation:
Section of Microbiology, Department of Ecology, Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, DK-1871, Frederiksberg, C, Denmark
*
*Author for correspondence Fax: +45 35 28 26 76 Email: jrg@kvl.dk
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Abstract

The fungus, Duddingtonia flagrans, is able to trap and kill free-living nematode larvae of the cattle parasite Cooperia oncophora when chlamydospores are mixed in cattle faeces. Isolates of Bacillus subtilis (two isolates), Pseudomonas spp. (three isolates) and single isolates of the fungal genera Alternaria, Cladosporium, Fusarium, Trichoderma and Verticillium were isolated from cattle faeces and shown to reduce D. flagrans growth on agar plates. When these isolates were added to cattle faeces containing D. flagrans and nematode larvae of C. oncophora, developing from eggs, none of the isolates reduced nematode mortality attributed to D. flagrans. Similarly, the coprophilic fungus Pilobolus kleinii, which cannot be cultivated on agar, also failed to suppress the ability of D. flagrans to trap and kill developing larvae of C. oncophora. Increasing chlamydospore doses of D. flagrans in faecal cultures resulted in higher nematode mortality. Thus, no evidence of interspecific or intraspecific competition was observed. The consequences of these findings are discussed.

Information

Type
Review Article
Information
Journal of Helminthology , Volume 78 , Issue 1 , March 2004 , pp. 41 - 46
Copyright
Copyright © Cambridge University Press 2004

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References

Cooke, R.C. (1969) Two nematode-trapping Hyphomycetes, Duddingtonia flagrans gen. et comb. nov. and Monacrosporium mutabilis sp. nov. Transactions of the British Mycological Society 53, 315–319.CrossRefGoogle Scholar
Githigia, S.M., Thamsborg, S.M., Larsen, M., Kyvsgaard, N.C. & Nansen, P. (1997) The preventive effect of the fungus Duddingtonia flagrans on trichostrongyle infections of lambs on pasture. International Journal for Parasitology 27, 931–939.CrossRefGoogle ScholarPubMed
Grønvold, J., Wolstrup, J., Larsen, M., Henriksen, S.A. & Nansen, P. (1993) Biological control of Ostertagia ostertagi by feeding selected nematode-trapping fungi to calves. Journal of Helminthology 67, 31–36.CrossRefGoogle ScholarPubMed
Grønvold, J., Nansen, P., Henriksen, S.A., Larsen, M., Wolstrup, J., Bresciani, J., Rawat, H. & Fribert, L. (1996) Induction of traps by Ostertagia ostertagi larvae, chlamydospore production and growth rate in the nematode-trapping fungus Duddingtonia flagrans . Journal of Helminthology 70, 291–297.CrossRefGoogle ScholarPubMed
Grønvold, J., Wolstrup, J., Nansen, P., Larsen, M., Henriksen, S.A., Bjørn, H., Kirchheiner, K., Lassen, K., Rawat, H. & Kristiansen, H.L. (1999) Biotic and abiotic factors influencing growth rate and production of traps by the nematode-trapping fungus Duddingtonia flagrans when induced by Cooperia oncophora larvae. Journal of Helminthology 73, 129–136.CrossRefGoogle Scholar
Henriksen, S.A. & Korsholm, H. (1983) A method for culture and recovery of gastrointestinal strongyle larvae. Nordisk Veterinærmedicin 35, 429–430.Google ScholarPubMed
Jørgensen, R.J., Rønne, H., Helsted, C. & Iskander, A.R. (1982) Spread of infective Dictyocaulus viviparus larvae in pasture and to grazing cattle: experimental evidence of the role of Pilobolus fungi. Veterinary Parasitology 10, 331–339.CrossRefGoogle ScholarPubMed
Larsen, M., Nansen, P., Wolstrup, J., Grønvold, J., Henriksen, S.A. & Zorn, A. (1995) Biological control of trichostrongyles in calves by the fungus Duddingtonia flagrans fed to animals under natural grazing conditions. Veterinary Parasitology 60, 321–330.CrossRefGoogle ScholarPubMed
Larsen, M., Nansen, P., Grøndahl, C., Thamsborg, S.M., Grønvold, J., Wolstrup, J., Henriksen, S.A. & Monrad, J. (1996) The capacity of the fungus Duddingtonia flagrans to prevent strongyle infections in foals on pasture. Parasitology 113, 1–6.CrossRefGoogle ScholarPubMed
Mankau, R. (1962) Soil fungistasis and nematophagus fungi. Phytopathology 52, 611–615.Google Scholar
Nansen, P., Larsen, M., Grønvold, J., Wolstrup, J., Zorn, A. & Henriksen, S.A. (1995) Prevention of clinical trichostrongylidosis in calves by strategic feeding with the predacious fungus Duddingtonia flagrans . Parasitology Research 81, 371–374.CrossRefGoogle ScholarPubMed
Nansen, P., Larsen, M., Roepstorff, A., Grønvold, J., Wolstrup, J. & Henriksen, S.A. (1996) Control of Oesophagostomum dentatum and Hyostrongylus rubidus in outdoor-reared pigs through daily feeding with the microfungus Duddingtonia flagrans . Parasitology Research 82, 580–584.CrossRefGoogle ScholarPubMed
Siegel, S. (1956) Nonparametric statistics for the behavioral sciences. 312 pp. McGraw-Hill Kogakusha, Ltd. Google Scholar
Wolstrup, J., Grønvold, J., Henriksen, S.A., Nansen, P., Larsen, M., Bøgh, H.O., Ilsøe, B. (1994) An attempt to implement the nematode-trapping fungus Duddingtonia flagrans in biological control of trichostrongyle infections of first year grazing calves. Journal of Helminthology 68, 175–180.CrossRefGoogle ScholarPubMed