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Surface Energy-mediated Protein and Osteoblast Responses on Nanostructured Stiff Surfaces

Published online by Cambridge University Press:  28 November 2012

Lei Yang ,
Maswazi Sihlabela ,
Brian W. Sheldon  and
Thomas J. Webster
Lei Yang
Affiliation:
Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou 215006, PR China School of Engineering, Brown University, Providence, RI 02912, USA
Maswazi Sihlabela
Affiliation:
School of Engineering, Brown University, Providence, RI 02912, USA
Brian W. Sheldon
Affiliation:
School of Engineering, Brown University, Providence, RI 02912, USA
Thomas J. Webster
Affiliation:
School of Engineering, Brown University, Providence, RI 02912, USA Department of Orthopaedics, Brown University, Providence, RI 02912, USA Current address: Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA
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Abstract

Nanostructured surfaces have demonstrated extraordinary capacity to influence protein adsorption and cellular responses, although the mechanisms behind such capacity are still not clear to date. In the present study, the role of surface energy associated with nanostructured stiff surfaces in modulating fibronectin and consequently osteoblast (OB, bone forming cells) responses was investigated. Nanocrystalline diamond (NCD) and submicron crystalline diamond (SMCD) films with controllable surface energy were prepared by microwave-enhanced plasma chemical vapor deposition (MPCVD) techniques. Fibronectin adsorption on the diamond films with varied surface energy values was measured via the enzyme-linked immunosorbent assay (ELISA) and the relationship between the surface energy and fibronectin adsorption was studied. OB aggregates (each containing 30∼50 cells) on the NCD with varied surface energy values were also studied. The results indicated that fibronectin adsorption on nanostructured surfaces was closely related to both surface energy and material microstructures, and osteoblast spreading and migration on stiff nanosurfaces are surface energy-driven processes.

Keywords

DiamondBoneSurface Chemistry
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1486: Symposium S4A – Nanotechnology-enhanced Biomaterials and Biomedical Devices , 2012 , imrc12-1486-s4a-o020
Copyright
Copyright © Materials Research Society 2012 

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References

REFERENCES

Yang, L., Zhang, L.J., Webster, T.J.. Adv Eng Mater 13, B197–B217 (2011).CrossRefGoogle Scholar
Chun, Y.W., Webster, T.J.. Ann Biomed Eng 37, 2034–2047 (2009).CrossRefGoogle Scholar
Zhang, L.J., Webster, T.J.. Nano Today 4, 66–80 (2009).CrossRefGoogle Scholar
Khang, D., Kim, S.Y., Liu-Snyder, P., Palmore, G.T., Durbin, S.M., Webster, T.J.. Biomaterials 28, 4756–4768 (2007).CrossRefGoogle Scholar
Wilson, C.J., Clegg, R.E., Leavesley, D.I., Pearcy, M.J.. Tissue Eng 11, 1–18 (2005).CrossRefGoogle Scholar
Yang, L., Li, Y.W., Sheldon, B.W., Webster, T.J.. J Mater Chem 22, 205–214 (2012).CrossRefGoogle Scholar
Lamour, G., Eftekhari-Bafrooei, A., Borguet, E., Soues, S., Hamraoui, A.. Biomaterials 31, 3762–3771 (2010).CrossRefGoogle Scholar
Yang, L., Sheldon, B.W., Webster, T.J.. Biomaterials 30, 3458–3465 (2009).CrossRefGoogle Scholar
Ryan, P.L., Foty, R.A., Kohn, J., Steinberg, M.S.. P Natl Acad Sci USA 98, 4323–4327 (2001).CrossRefGoogle Scholar
Goeting, C.H., Marken, F., Gutierrez-Sosa, A., Compton, R.G., Foord, J.S.. Diam Relat Mater 9, 390–396 (2000).CrossRefGoogle Scholar
Ostrovskaya, L., Perevertailo, V., Ralchenko, V., Dementjev, A., Loginova, O.. Diam Relat Mater 11, 845–850 (2002).CrossRefGoogle Scholar
Gruen, D.M.. Annu Rev Mater Sci 29, 211–259 (1999).CrossRefGoogle Scholar