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Fast Real-Time PCR assay for detection of Tetramicra brevifilum in cultured turbot

Published online by Cambridge University Press:  15 October 2012

MERCEDES ALONSO ,
FÁTIMA C. LAGO ,
MARÍA GÓMEZ-REINO ,
JACOBO FERNÁNDEZ ,
IRIS MARTÍN ,
JUAN M. VIEITES  and
MONTSERRAT ESPIÑEIRA
MERCEDES ALONSO
Affiliation:
Research Department of Genomics and Proteomics Applied to the Marine and Food Industry, ANFACO-CECOPESCA, Vigo, 36310 Pontevedra, Spain
FÁTIMA C. LAGO
Affiliation:
Research Department of Genomics and Proteomics Applied to the Marine and Food Industry, ANFACO-CECOPESCA, Vigo, 36310 Pontevedra, Spain
MARÍA GÓMEZ-REINO
Affiliation:
Research Department of Aquaculture, ANFACO-CECOPESCA, Vigo, 36310 Pontevedra, Spain
JACOBO FERNÁNDEZ
Affiliation:
INSUIÑA S.L., Xove, Lugo, Spain
IRIS MARTÍN
Affiliation:
INSUIÑA S.L., Xove, Lugo, Spain
JUAN M. VIEITES
Affiliation:
Research Department of Genomics and Proteomics Applied to the Marine and Food Industry, ANFACO-CECOPESCA, Vigo, 36310 Pontevedra, Spain
MONTSERRAT ESPIÑEIRA*
Affiliation:
Research Department of Genomics and Proteomics Applied to the Marine and Food Industry, ANFACO-CECOPESCA, Vigo, 36310 Pontevedra, Spain
*
*Corresponding author: Research Department of Genomics and Proteomics Applied to the Marine and Food Industry, ANFACO-CECOPESCA, Vigo, 36310 Pontevedra, Spain. Tel: +31 34 986 469 301. Fax: +31 34 986 469 269. E-mail: montse@anfaco.es
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Summary

Global aquaculture production of turbot has rapidly increased worldwide in the last decade and it is expected to have even bigger growth in the next years due to new farms operating. The losses caused by pathogen infections have grown at the same time as the production of this species. Parasitological infections are among the main relevant pathologies associated with its culture and produce serious losses in aquaculture, reduce the growth rate in fish and may lead to unmarketable fish due to skeletal muscle abnormalities in cases with high intensity of infection. The microsporidian parasite Tetramicra brevifilum causes severe infections and generates major losses in farmed turbot. Infections are difficult to control due to spore longevity and its direct transmission. To facilitate the infection management, an effective tool for fast detection and identification of T. brevifilum is needed. This study provides a molecular methodology of fast Real-Time PCR for T. brevifilum detection to the aquaculture industry, useful for routine control of T. brevifilum at turbot farms. The method is characterized by its high specificity and sensitivity, and it can be applied to cultured turbot for parasite detection regardless of the life-cycle stage of the pathogen or the infection intensity.

Keywords

aquaculturefast Real-Time PCRTetramicra brevifilummicrosporidianturbot diseasedetection
Type
Research Article
Information
Parasitology , Volume 140 , Issue 3 , March 2013 , pp. 338 - 342
Copyright
Copyright © Cambridge University Press 2012

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