Hostname: page-component-76dd75c94c-h9cmj Total loading time: 0 Render date: 2024-04-30T07:47:56.564Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of feeding lambs with a tanniferous shrub (rockrose) and a vegetable oil blend on fatty acid composition of meat lipids

Published online by Cambridge University Press:  16 June 2016

A. Francisco ,
S. P. Alves ,
P. V. Portugal ,
V. M. R. Pires ,
M. T. Dentinho ,
C. M. Alfaia ,
E. Jerónimo ,
J. A. M. Prates ,
J. Santos-Silva  and
R. J. B. Bessa
A. Francisco
Affiliation:
Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal Faculdade de Medicina Veterinária, Universidade de Lisboa (ULisboa), 1300-477 Lisbon, Portugal Unidade Estratégica de Investigação e Serviços em Produção e Saúde Animal, Instituto Nacional de Investigação Agrária e Veterinária (INIAV), 2005-048 Vale de Santarém, Portugal
S. P. Alves
Affiliation:
Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal Faculdade de Medicina Veterinária, Universidade de Lisboa (ULisboa), 1300-477 Lisbon, Portugal
P. V. Portugal
Affiliation:
Unidade Estratégica de Investigação e Serviços em Produção e Saúde Animal, Instituto Nacional de Investigação Agrária e Veterinária (INIAV), 2005-048 Vale de Santarém, Portugal
V. M. R. Pires
Affiliation:
Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal Faculdade de Medicina Veterinária, Universidade de Lisboa (ULisboa), 1300-477 Lisbon, Portugal
M. T. Dentinho
Affiliation:
Unidade Estratégica de Investigação e Serviços em Produção e Saúde Animal, Instituto Nacional de Investigação Agrária e Veterinária (INIAV), 2005-048 Vale de Santarém, Portugal
C. M. Alfaia
Affiliation:
Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal Faculdade de Medicina Veterinária, Universidade de Lisboa (ULisboa), 1300-477 Lisbon, Portugal
E. Jerónimo
Affiliation:
Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo (CEBAL), Instituto Politécnico de Beja (IPBeja), 7801-908 Beja, Portugal
J. A. M. Prates
Affiliation:
Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal Faculdade de Medicina Veterinária, Universidade de Lisboa (ULisboa), 1300-477 Lisbon, Portugal
J. Santos-Silva
Affiliation:
Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal Unidade Estratégica de Investigação e Serviços em Produção e Saúde Animal, Instituto Nacional de Investigação Agrária e Veterinária (INIAV), 2005-048 Vale de Santarém, Portugal
R. J. B. Bessa*
Affiliation:
Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal Faculdade de Medicina Veterinária, Universidade de Lisboa (ULisboa), 1300-477 Lisbon, Portugal
*
†E-mail: rjbbessa@fmv.ulisboa.pt
Get access

Abstract

The effects of feeding Cistus ladanifer (Cistus) and a blend of soybean and linseed oil (1 : 2 vol/vol) on fatty acid (FA) composition of lamb meat lipids and messenger RNA (mRNA) expression of desaturase enzymes was assessed. In total, 54 male lambs were randomly assigned to 18 pens and to nine diets, resulting from the combination of three inclusion levels of Cistus (50 v. 100 v. 200 g/kg of dry matter (DM)) and three inclusion levels of oil (0 v. 40 v. 80 g/kg of DM). The forage-to-concentrate ratio of the diets was 1 : 1. Longissimus muscle lipids were extracted, fractionated into neutral (NL) and polar lipid (PL) and FA methyl esters obtained and analyzed by GLC. The expression of genes encoding Δ5, Δ6 and Δ9 desaturases (fatty acid desaturase 1 (FADS1), fatty acid desaturase 2 (FADS2) and stearoyl CoA desaturase (SCD)) was determined. Intramuscular fat, NL and PL contents were not affected by oil or Cistus. Oil supplementation reduced (P<0.05) 16:0, c9-16:1, 17:0, c9-17:1 and c9-18:1 FA and increased (P<0.05) 18:2n-6, 18:3n-3 and the majority of biohydrogenation intermediates in NL. Cistus alone had few effects on FA of NL but interacted with oil (P<0.05) by increasing t10-18:1,t10,t12-18:2,t10,c12-18:2 and t7,c9-18:2. The t10-/t11-18:1 ratio increased with both Cistus and oil levels. The c9, t11-18:2 did not increase (P<0.05) with both oil and Cistus dietary inclusion. Oil reduced c9-16:1, 17:0, c9-17:1,c9-18:1, 20:4n-6, 22:4n-6 and 20:3n-9 proportions in PL, and increased 18:2n-6, 18:3n-3, 20:3n-3 and of most of the biohydrogenation intermediates. The Cistus had only minor effects on FA composition of PL. Cistus resulted in a reduction (P<0.05) of 20:5n-3 and 22:6n-3 in the meat PL. The expression level of SCD mRNA increased (P=0.015) with Cistus level, although a linear relationship with condensed tannins intake (P=0.11) could not be established. FADS1 mRNA expressed levels increased linearly (P=0.019) with condensed tannins intake. In summary, the inclusion of Cistus and oil in 1 : 1 forage-to-concentrate ratio diets resulted in a large increase in t10-18:1 and no increase in c9,t11-18:2 or n-3 long chain poor in polyunsaturated fatty acids in lamb meat.

Keywords

tanninslipid supplementationbiohydrogenationTrans-10 shiftfatty acid desaturases
Type
Research Article
Information
animal , Volume 10 , Issue 12 , December 2016 , pp. 2061 - 2073
Copyright
© The Animal Consortium 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aldai, N, de Renobales, M, Barron, LJR and Kramer, JKG 2013. What are the trans fatty acids issues in foods after discontinuation of industrially produced trans fats? Ruminant products, vegetable oils, and synthetic supplements. European Journal of Lipid Science and Technology 115, 1378–1401.Google Scholar
Alvarenga, TIRC, Chen, Y, Furusho-Garcia, IF, Perez, JRO and Hopkins, DL 2015. Manipulation of omega-3 PUFAs in lamb: phenotypic and genotypic views. Comprehensive Reviews in Food Science and Food Safety 14, 189–204.Google Scholar
Alves, SP and Bessa, RJB 2009. Comparison of two gas-liquid chromatograph columns for the analysis of fatty acids in ruminant meat. Journal of Chromatography A 1216, 5130–5139.Google Scholar
Alves, SP and Bessa, RJB 2014. The trans-10,cis-15 18:2: a missing intermediate of trans-10 shifted rumen biohydrogenation pathway? Lipids 49, 527–541.CrossRefGoogle Scholar
Bessa, RJB, Alves, SP, Jerónimo, E, Alfaia, CM, Prates, JAM and Santos-Silva, J 2007. Effect of lipid supplements on ruminal biohydrogenation intermediates and muscle fatty acids in lambs. European Journal of Lipid Science and Technology 109, 868–878.CrossRefGoogle Scholar
Bessa, RJB, Alves, SP and Santos-Silva, J 2015. Constraints and potentials for the nutritional modulation of the fatty acid composition of ruminant meat. European Journal of Lipid Science and Technology 117, 1325–1344.Google Scholar
Bessa, RJB, Portugal, PV, Mendes, IA and Santos-Silva, J 2005. Effect of lipid supplementation on growth performance, carcass and meat quality and fatty acid composition of intramuscular lipids of lambs fed dehydrated lucerne or concentrate. Livestock Production Science 96, 185–194.Google Scholar
Bessa, RJB, Santos-Silva, J, Ribeiro, JMR and Portugal, AV 2000. Reticulo-rumen biohydrogenation and the enrichment of ruminant edible products with linoleic acid conjugated isomers. Livestock Production Science 63, 201–211.Google Scholar
Buccioni, A, Pauselli, M, Viti, C, Minieri, S, Pallara, G, Roscini, V, Rapaccini, S, Marinucci, MT, Lupi, P, Conte, G and Mele, M 2015. Milk fatty acid composition, rumen microbial population, and animal performances in response to diets rich in linoleic acid supplemented with chestnut or quebracho tannins in dairy ewes. Journal of Dairy Science 98, 1145–1156.Google Scholar
da Costa, ASH, Bessa, RJB, Pires, VMR, Rolo, EA, Pinto, RMA, Fontes, CMGA and Prates, JAM 2014. Is hepatic lipid metabolism of beef cattle influenced by breed and dietary silage level? BMC Veterinary Research 10, 65.Google Scholar
Daniel, ZCTR, Wynn, RJ, Salter, AM and Buttery, PJ 2004. Differing effects of forage and concentrate diets on the oleic acid and conjugated linoleic acid content of sheep tissues: the role of stearoyl-CoA desaturase. Journal of Animal Science 82, 747–758.CrossRefGoogle ScholarPubMed
Food and Agriculture Organization (FAO) 2010. Fats and fatty acids in human nutrition (FAO report of an expert consultation). FAO, Rome, Italy.Google Scholar
Francisco, A, Dentinho, MT, Alves, SP, Portugal, PV, Fernandes, F, Sengo, S, Jerónimo, E, Oliveira, MA, Costa, P, Sequeira, A, Bessa, RJB and Santos-Silva, J 2015. Growth performance, carcass and meat quality of lambs supplemented with increasing levels of a tanniferous bush (Cistus ladanifer L.) and vegetable oils. Meat Science 100, 275–282.Google Scholar
Gruffat, D, Remond, C, Durand, D, Loreau, O and Bauchart, D 2008. 9cis, 11trans conjugated linoleic acid (CLA) is synthesised and desaturated into conjugated 18:3 in bovine adipose tissues. Animal 2, 645–652.CrossRefGoogle Scholar
Guerreiro, O, Alves, SP, Duarte, MF, Bessa, RJB and Jerónimo, E 2015. Cistus ladanifer L. Shrub is rich in saturated and branched chain fatty acids and their concentration increases in the mediterranean dry season. Lipids 50, 493–501.Google Scholar
Guerreiro, O, Dentinho, MT, Moreira, OC, Guerra, AR, Ramos, PAB, Bessa, RJB, Duarte, MF and Jerónimo, E 2016. Potential of Cistus ladanifer L. (rockrose) in small ruminant diets – effect of season and plant age on chemical composition, in vitro digestibility and antioxidant activity. Grass and Forage Science, doi: 10.1111/gfs.12188.Google Scholar
Jerónimo, E, Alves, SP, Alfaia, CM, Prates, JAM, Santos-Silva, J and Bessa, RJB 2011. Biohydrogenation intermediates are differentially deposited between polar and neutral intramuscular lipids of lambs. European Journal of Lipid Science and Technology 113, 924–934.CrossRefGoogle Scholar
Jerónimo, E, Alves, SP, Dentinho, MTP, Martins, SV, Prates, JAM, Vasta, V, Santos-Silva, J and Bessa, RJB 2010. Effect of grape seed extract, Cistus ladanifer L., and vegetable oil supplementation on fatty acid composition of abomasal digesta and intramuscular fat of lambs. Journal of Agricultural and Food Chemistry 58, 10710–10721.Google Scholar
Khiaosa-Ard, R, Bryner, SF, Scheeder, MRL, Wettstein, HR, Leiber, F, Kreuzer, M and Soliva, CR 2009. Evidence for the inhibition of the terminal step of ruminal α-linolenic acid biohydrogenation by condensed tannins. Journal of Dairy Science 92, 177–188.Google Scholar
Kronberg, SL, Scholljegerdes, EJ, Barcelo-Coblijn, G and Murphy, EJ 2007. Flaxseed treatments to reduce biohydrogenation of α-linolenic acid by rumen microbes in cattle. Lipids 42, 1105–1111.Google Scholar
Livak, KJ and Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25, 402–408.CrossRefGoogle Scholar
Moore, JH and Christie, WW 1984. Digestion, absorption and transport of fats in ruminant animals. In Fats in Animal Nutrition (ed. J Wiseman), pp. 123–149. Butterwords, London, UK.Google Scholar
Palmquist, DL, St-Pierre, N and McClure, KE 2004. Tissue fatty acid profiles can be used to quantify endogenous rumenic acid synthesis in lambs. Journal of Nutrition 134, 2407–2414.Google Scholar
Rana, MS, Tyagi, A, Hossain, SA and Tyagi, AK 2012. Effect of tanniniferous Terminalia chebula extract on rumen biohydrogenation, Δ9-desaturase activity, CLA content and fatty acid composition in longissimus dorsi muscle of kids. Meat Science 90, 558–563.Google Scholar
Rosa, HJD, Rego, OA, Silva, CCG, Alves, SP, Alfaia, CMM, Prates, JAM and Bessa, RJB 2014. Effect of corn supplementation of grass finishing of Holstein bulls on fatty acid composition of meat lipids. Journal of Animal Science 92, 3701–3714.Google Scholar
Shingfield, KJ, Bonnet, M and Scollan, ND 2013. Recent developments in altering the fatty acid composition of ruminant-derived foods. Animal 7, 132–162.Google Scholar
Shingfield, KJ and Wallace, RJ 2014. Synthesis of conjugated linoleic acid in ruminants and humans. In Conjugated linoleic acids and conjugated vegetable oils (ed. B Sels and A Philippaerts), pp. 1–65. The Royal Society of Chemistry, London, UK.Google Scholar
Smith, SB, Kawachi, H, Choi, CB, Choi, CW, Wu, G and Sawyer, JE 2009. Cellular regulation of bovine intramuscular adipose tissue development and composition. Journal of Animal Science 87, E72–E82.Google Scholar
Sukhija, PS and Palmquist, DL 1988. Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. Journal of Agricultural and Food Chemistry 36, 1202–1206.Google Scholar
Toral, PG, Hervas, G, Belenguer, A, Bichi, E and Frutos, P 2013. Effect of the inclusion of quebracho tannins in a diet rich in linoleic acid on milk fatty acid composition in dairy ewes. Journal of Dairy Science 96, 431–439.Google Scholar
Vasta, V and Bessa, RJB 2012. Manipulating ruminal biohydrogenation by the use of plants bioactive compounds. In Dietary phytochemistry and microbes (ed. AK Patra), pp. 263–284. Springer, Dordrecht, The Netherlands.Google Scholar
Vasta, V, Mele, M, Serra, A, Scerra, M, Luciano, G, Lanza, M and Priolo, A 2009b. Metabolic fate of fatty acids involved in ruminal biohydrogenation in sheep fed concentrate or herbage with or without tannins. Journal of Animal Science 87, 2674–2684.Google Scholar
Vasta, V, Priolo, A, Scerra, M, Hallett, KG, Wood, JD and Doran, O 2009a. Δ9 desaturase protein expression and fatty acid composition of longissimus dorsi muscle in lambs fed green herbage or concentrate with or without added tannins. Meat Science 82, 357–364.Google Scholar
Wynn, RJ, Daniel, ZCTR, Flux, CL, Craigon, J, Salter, AM and Buttery, PJ 2006. Effect of feeding rumen-protected conjugated linoleic acid on carcass characteristics and fatty acid composition of sheep tissues. Journal of Animal Science 84, 3440–3450.Google Scholar
Supplementary material: PDF

Francisco supplementary material

Table S1

Download Francisco supplementary material(PDF)
PDF 273.7 KB