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Comparison of various semen extenders and addition of prostaglandin F2α on pregnancy rate in cows

Published online by Cambridge University Press:  11 November 2015

A. M. Abdussamad ,
J. Detterer ,
M. Gauly  and
W. Holtz
A. M. Abdussamad
Affiliation:
Department of Animal Science, Georg-August-University Goettingen, Albrecht-Thaer-Weg 3, 37075 Goettingen, Germany
J. Detterer
Affiliation:
Verein Ostfriesischer Stammviehzuechter, AI and ET Center, 26770 Georgsheil, Germany
M. Gauly
Affiliation:
Department of Animal Science, Georg-August-University Goettingen, Albrecht-Thaer-Weg 3, 37075 Goettingen, Germany
W. Holtz*
Affiliation:
Department of Animal Science, Georg-August-University Goettingen, Albrecht-Thaer-Weg 3, 37075 Goettingen, Germany
*
†E-mail: wholtz@gwdg.de
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Abstract

This investigation comprises three trials. Trial 1 consists of an in vitro comparison of three semen extenders: two egg yolk based (customized Tris-egg yolk-glycerol and Triladyl®), the third (AndroMed®) soybean lecithin based. With regard to post-thaw motility, the phytoextender AndroMed® proved to be superior (59±3% v. 53±2% and 53±2%, P<0.05). It had earlier been shown that addition of the commercial prostaglandin F2α preparation Dinolytic® before freezing compromises post-thaw motility; therefore, in Trial 2, Dinolytic® was added after thawing. Frozen-thawed spermatozoa tolerated addition of Dinolytic® at a concentration of 30% (v/v). In Trial 3, cows were inseminated using straws in which diluted semen and Dinolytic® were frozen in the same straw, separated by an air bubble, so intermingling could only take place in the course of insemination. Pregnancy rates at Dinolytic® dosages of 0%, 30% or 60% amounted to 44%, 41% and 56%, respectively (P>0.05), a result that encourages a large-scale field study, which is envisioned.

Keywords

cattleartificial inseminationspermatozoan motilitysemen extenderprostaglandin F2α
Type
Research Article
Information
animal , Volume 10 , Issue 4 , April 2016 , pp. 655 - 659
Copyright
© The Animal Consortium 2015 

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