Hostname: page-component-848d4c4894-wg55d Total loading time: 0 Render date: 2024-05-16T03:35:19.760Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of a bovine colostrum whey supplementation on growth performance, faecal Escherichia coli population and systemic immune response of piglets at weaning

Published online by Cambridge University Press:  15 April 2008

C. Boudry ,
J.-P. Dehoux ,
J. Wavreille ,
D. Portetelle ,
A. Théwis  and
A. Buldgen
C. Boudry*
Affiliation:
Animal Husbandry Unit, Gembloux Agricultural University, Passage des Déportés 2, 5030 Gembloux, Belgium
J.-P. Dehoux
Affiliation:
Experimental Surgery Unit, Faculty of Medicine, Catholic University of Louvain, Avenue Hippocrate 55/70, 1200 Brussels, Belgium
J. Wavreille
Affiliation:
Department of Animal Production and Nutrition, Agricultural Research Centre, Ministry of Walloon Region, rue de Liroux 8, 5030 Gembloux, Belgium
D. Portetelle
Affiliation:
Animal and Microbial Biology Unit, Gembloux Agricultural Unit, Passage des Déportés 2, 5030 Gembloux, Belgium
A. Théwis
Affiliation:
Animal Husbandry Unit, Gembloux Agricultural University, Passage des Déportés 2, 5030 Gembloux, Belgium
A. Buldgen
Affiliation:
Animal Husbandry Unit, Gembloux Agricultural University, Passage des Déportés 2, 5030 Gembloux, Belgium
*
E-mail: boudry.c@fsagx.ac.be
Get access

Abstract

This study examined the effect of a bovine colostrum whey supplementation on growth performance, feed intake, faecal Escherichiacoli population and systemic immune response of piglets at weaning. A total of 96 piglets weaned at 26 ± 2 days of age were assigned for 4 weeks to one of the two treatments: (1) the control (commercial diet with bovine milk whey powder) and (2) the colostrum (commercial diet with freeze-dried bovine colostrum whey) treatments. The two supplements were incorporated in the diet at a level of 20 g/kg during the first 2 weeks after weaning and lowered to a level of 10 g/kg for the next 2 weeks. BW and feed intake were measured weekly. Faecal E. coli counts were determined weekly on specific culture media. Blood samples were collected weekly and submitted to a cell counter analyser for their main components (red and white blood cells, platelets) and flow cytometry was used to determine the lymphocyte population (B, T, Th and Tc). Finally, total seric immunoglobulin (IgM, IgG and IgA) concentrations were determined by the ELISA method. During the first week of the trial, the piglets from the colostrum treatment had improved average daily gain (170 g/day v. 81 g/day, P < 0.001), average daily feed intake (346 g/day v. 256 g/day, P = 0.03) and feed efficiency (BW gain/feed intake) (0.48 v. 0.31, P = 0.04). The pigs fed the colostrum treatment had also a 25% increase in circulating IgA (P = 0.03) compared with the control treatment the first week. It is concluded that a distribution of bovine colostrum whey (20 g/kg diet) during the first week post-weaning induces a systemic IgA response and has a beneficial action on growth performances and feed efficiency.

Keywords

bovine colostrumE. coliimmunoglobulinpigsweaning
Type
Full Paper
Information
animal , Volume 2 , Issue 5 , May 2008 , pp. 730 - 737
Copyright
Copyright © The Animal Consortium 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Association of Official Analytical Chemists 1990. Official methods of analysis, 15th edition. AOAC, Arlington, VA, USA.Google Scholar
Bianchi, ATJ, Scholten, JW, Moonen Leusen, BHW, Boersma, WJA 1999. Development of the natural response of immunoglobulin secreting cells in the pig as a function of organ, age and housing. Developmental and Comparative Immunology 23, 511–520.CrossRefGoogle ScholarPubMed
Boudry, C, Buldgen, A, Portetelle, D, Collard, A, Théwis, A, Dehoux, J-P 2007. Effects of oral supplementation with bovine colostrum on the immune system of weaned piglets. Research in Veterinary Science 83, 91–101.CrossRefGoogle ScholarPubMed
Carbonare, SB, Silva, ML, Palmeira, P, Carneiro-Sampaio, MM 1997. Human colostrum IgA antibodies reacting to enteropathogenic E. coli antigens and their persistence in the faeces of a breastfed infant. Journal of Diarrhoea Disease Research 15, 53–58.Google Scholar
Chun, SK, Nam, MS, Goh, JS, Kim, WS, Han, YH, Kim, PH 2004. Kinetics and biological function of transforming growth factor-β isoforms in bovine and human colostrum. Journal of Microbiology and Biotechnology 14, 1267–1274.Google Scholar
Coffey, RD, Cromwell, GL 2001. Use of spray-dried animal plasma in diets for weanling pigs. Pig News and Information 22, 39N–48N.Google Scholar
Cross, ML, Gill, HS 2000. Immunomodulatory properties of milk. British Journal of Nutrition 84, S81–S89.CrossRefGoogle Scholar
Elfstrand, L, Lindmark-Mansson, H, Paulsson, M, Nyberg, L, Akesson, B 2002. Immunoglobulins, growth factors and growth hormone in bovine colostrum and the effects of processing. International Dairy Journal 12, 879–887.CrossRefGoogle Scholar
Erdei, J, Forsgren, A, Naidu, AS 1994. Lactoferrin binds to porins Ompf and Ompc in E. coli. Infection and Immunity 62, 1236–1240.CrossRefGoogle Scholar
Faisant, N, Planchot, V, Kozlowski, F, Pacouret, M-P, Colonna, P, Champ, M 1995. Resistant starch determination adapted to products containing high level of resistant starch. Sciences des Aliments 15, 83–89.Google Scholar
Gill, HS, Rutherfurd, KJ 1998. Immunomodulatory properties of bovine milk. Bulletin of the International Dairy Federation 336, 31–35.Google Scholar
Honorio-Franca, AC, Carvalho, MP, Isaac, L, Trabulsi, LR, Carneiro-Sampaio, MM 1997. Colostral mononuclear phagocytes are able to kill enteropathogenic E. coli opsonised with colostral IgA. Scandinavian Journal of Immunology 46, 59–66.CrossRefGoogle ScholarPubMed
Hopwood, DE, Hampson, DJ 2003. Interactions between the intestinal microflora, diet and diarrhoea, and their influences on piglet health in the immediate post-weaning period. In Weaning the pig. Concepts and consequences (ed. JR Pluske, J Le Dividich and MWA Verstegen), pp. 199–218. Wageningen Academic Publishers, Wageningen, The Netherlands.Google Scholar
Huguet, A, Sève, B, Le Dividich, J, Le Huërou-Luron, I 2006. Effects of a bovine colostrum-supplemented diet on some gut parameters in weaned piglets. Reproduction, Nutrition, Development 46, 167–178.Google Scholar
Huguet, A, Le Normand, L, Fauquant, J, Kaeffer, B, Le Huërou-Luron, I 2007. Influence of bovine colostrum on restoration of intestinal mucosa in weaned piglets. Livestock Science 108, 20–22.CrossRefGoogle Scholar
Katouli, M, Lund, A, Wallgren, P, Kühn, I, Soderlind, O, Mollby, R 1995. Phenotypic characterization of intestinal E. coli of pigs during suckling, postweaning, and fattening periods. Applied and Environmental Microbiology 61, 778–783.CrossRefGoogle ScholarPubMed
King, MR, Morel, PCH, James, EAC, Hendriks, WH, Pluske, JR, Skilton, R, Skilton, G 2001. Inclusion of colostrum powder and bovine plasma in starter diets increases voluntary feed intake. In Manipulating pig production VIII, p. 213. Australian Pig Science Association, Werribee, Australia.Google Scholar
King, RH, Kelly, D, Morel, PCH, Pluske, JR 2003. Aspects of intestinal immunity in the pig around weaning. In Weaning the pig. Concepts and consequences (ed. JR Pluske, J Le Dividich and MWA Verstegen), pp. 219–258. Wageningen Academic Publishers, Wageningen, The Netherlands.Google Scholar
Le Huërou-Luron, I, Marion, J, Bebin, K, Thomas, F, Ganier, P, Thibault, JN, Le Dividich, J 2003. Supplementation of a weaning diet with a bovine colostrum extract minimise post-weaning duodenal structural, but not functional, alterations in 7-d old piglets. In The 9th International Symposium on Digestive Physiology in Pigs (ed. RO Ball), pp. 238–240. University of Alberta, Edmonton, Alberta, Canada.Google Scholar
Le Huërou-Luron, I, Hughet, A, Callarec, J, Leroux, T, Le Dividich, J 2004. La supplémentation de l’aliment de sevrage en colostrum bovin améliore l’ingestion et les performances zootechniques chez les porcelets au sevrage. Journées de la Recherche Porcine en France 36, 33–38.Google Scholar
McGee, HS, Agrawal, DK 2006. Th2 cells in the pathogenesis of airway remodelling: regulatory T cells a plausible panacea for asthma. Immunologic Research 35, 219–232.Google Scholar
Melin, L, Mattson, S, Katouli, M, Wallgren, P 2004. Development of post-weaning diarrhoea in piglets. Relation to presence of E. coli strains and Rotavirus. Journal of Veterinary Medicine Series B 51, 12–22.CrossRefGoogle ScholarPubMed
Pakkanen, R, Aalto, J 1997. Growth factors and antimicrobial factors of bovine colostrum. International Dairy Journal 7, 285–297.Google Scholar
Pierce, JL, Cromwell, GL, Lindemann, MD, Russell, LE, Weaver, EM 2005. Effects of spray-dried animal plasma and immunoglobulins on performance of early weaned piglets. Journal of Animal Science 83, 2876–2885.Google Scholar
Pluske, JR, Hampson, DJ, Williams, IH 1997. Factors influencing the structure and function of the small intestine in the weaned pig: a review. Livestock Production Science 52, 215–236.CrossRefGoogle Scholar
Pluske, JR, Pearson, G, Morel, PCH, King, MR, Skilton, G, Skilton, R 1999. A bovine colostrum product in a weaner diet increases growth and reduces day to slaughter. In Manipulating pig production, VII, p 256. Australian Pig Science Association, Werribee, Australia.Google Scholar
Reiter, B 1985. The biological significance of the non-immunoglobulin protective proteins in milk: lysozyme, lactoferrin, lactoperoxydase. Development in Dairy Chemistry 3, 281–336.CrossRefGoogle Scholar
Romagnani, S 1991. Type 1 T helper and Type 2 T helper cells: functions, regulation and role in protection and disease. Journal of Clinical Laboratory Research 21, 152–158.Google ScholarPubMed
Saito, H, Miyakawa, H, Tamura, Y, Shimamura, S, Tomita, M 1991. Potent bactericidal activity of bovine lactoferrin hydrolysate produced by heat treatment at acidic pH. Journal of Dairy Science 74, 3724–3730.Google Scholar
Vaerman, JP, Langendries, A, Pabst, R, Rothkötter, HJ 1997. Contribution of serum IgA to intestinal lymph IgA, and vice versa, in minipigs. Veterinary Immunology and Immunopathology 58, 301–308.CrossRefGoogle ScholarPubMed
Van Vlasselaer, P, Punnonen, J, De Vries, JE 1992. Transforming growth factors-β directs IgA switching in human B cells. Journal of Immunology 148, 2062–2067.CrossRefGoogle ScholarPubMed
Xu, RJ, Sangild, PT, Zhang, YQ, Zhang, SH 2002. Bioactive compounds in porcine colostrum and milk and their effects on intestinal development in neonatal pigs. In Biology of the intestine in growing animals (ed. R Zabielsky, PC Gregory and B Weström), pp. 169–192. Elsevier Science B.V., Amsterdam, The Netherlands.CrossRefGoogle Scholar
Yamauchi, K, Toimita, M, Giehl, TJ, Ellison, RT 1993. Antibacterial activity of lactoferrin and a pepsin-derived lactoferrin peptide fragment. Infection and Immunity 61, 719–728.CrossRefGoogle Scholar