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Effects of dietary inclusion of citrus pulp and rockrose soft stems and leaves on lamb meat quality and fatty acid composition

  • A. Francisco (a1) (a2) (a3), S. P. Alves (a1) (a2), P. V. Portugal (a3), M. T. Dentinho (a1) (a3), E. Jerónimo (a4), S. Sengo (a4), J. Almeida (a3), M. C. Bressan (a1) (a3), V. M. R. Pires (a1) (a2), C. M. Alfaia (a1) (a2), J. A. M. Prates (a1) (a2), R. J. B. Bessa (a1) (a2) and J. Santos-Silva (a1) (a3)...
Abstract

Meat from lambs finished with high-starch diets often contains low concentration of vaccenic (t11-18:1) and rumenic (c9,t11-18:2) acids and high concentration of t10-18:1. We hypothesized that replacing cereals by dehydrated citrus pulp (DCP) and the inclusion of tanniferous feed sources in oil supplemented diets might reduce the accumulation of t10-18:1 and increase the t11-18:1 and c9,t11-18:2 in lamb meat, without affecting the productive performance. In total, 32 lambs were assigned to four diets which combine two factors: basal diet (BD) (cereals v. DCP) and Cistus ladanifer (CL) (0 v. 150 g/kg dry matter). Feed intake, average daily weight gain and carcass traits were not affected by treatments, except for dressing percentage that was reduced with DCP (P=0.046). Both DCP and C. ladanifer reduced tenderness and juiciness of meat, and C. ladanifer also reduced (P<0.001) meat overall acceptability. Intramuscular fat and the concentration of saturated and polyunsaturated fatty acids (FA) were not affected (P>0.05) by diets. However, DCP increased the proportions of odd-chain FA (P=0.005) and several minor biohydrogenation (BH) intermediates in meat lipids. C. ladanifer had few effects on meat FA profile. The proportions of t11-18:1 and c9,t11-18:2 were high in all diets (5.4% and 1.5% of total FA, respectively) and were not influenced by the treatments. Basal diet and CL showed some significant interactions concerning FA composition of intramuscular fat. In diets without C. ladanifer, replacement of cereals by DCP increased the 18:0 (P<0.05) and decreased t10,c12-18:2 (P<0.05), t10-18:1 (P<0.10) and t10-/t11-18:1 ratio (P<0.10) with a large reduction of the individual variation for t10-18:1 and of t10-/t11-18:1 ratio. Combined with cereals, C. ladanifer increased 18:0 and reduced the BH intermediates in meat. Replacement of cereals by DCP seems to promote a more predictable FA profile in lamb meat, reducing the risk of t10-shifted BH pathways in the rumen.

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Corresponding author
E-mail: rjbbessa@fmv.ulisboa.pt
References
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Alvarenga, TIRC, Chen, Y, Furusho-Garcia, IF, Perez, JRO and Hopkins, DL 2015. Manipulation of omega-3 PUFAs in lamb: phenotypic and genotypic views. Compreensive Reviews in Food Science and Food Safety 14, 189204.
Alves, SP and Bessa, RJB 2009. Comparison of two gas-liquid chromatograph columns for the analysis of fatty acids in ruminant meat. Journal of Chromatography A 1216, 51305139.
Bampidis, VA and Robinson, PH 2006. Citrus by-products as ruminant feeds: a review. Animal Feed Science and Technology 128, 175217.
Bessa, RJB, Alves, SP, Jerónimo, E, Alfaia, CM, Prates, JAM and Santos-Silva, J 2007. Effect of lipid supplements on ruminal biohydrogenation intermediates and muscle fatty acids in lambs. European Journal of Lipid Science Technology 109, 868878.
Bessa, RJB, Alves, SP and Santos-Silva, J 2015. Constraints and potentials for the nutritional modulation of the fatty acid composition of ruminant meat. European Journal of Lipid Science and Technology 117, 13251344.
Caparra, P, Foti, F, Scerra, M, Sinatra, MC and Scerra, V 2007. Solar-dried citrus pulp as an alternative energy source in lamb diets: effects on growth and carcass and meat quality. Small Ruminant Research 68, 303311.
da Costa, ASH, Bessa, RJB, Pires, VMR, Rolo, EA, Pinto, RMA, Fontes, CMGA and Prates, JAM 2014. Is hepatic lipid metabolism of beef cattle influenced by breed and dietary silage level? BMC Veterinary Research 10, 65.
Eifert, ED, Lana, RD, Lanna, DPD, Teixeira, RMA, Arcuri, PB, Leao, MI, de Oliveira, MVM and Valadares, SD 2006. Milk fatty acid profile and milk conjugated linoleic acid content of dairy cows fed diets with different carbohydrate sources with or without soybean oil supplementation. Revista Brasileira de Zootecnia 35, 18291837.
Francisco, A, Alves, SP, Portugal, PV, Pires, VMR, Dentinho, MT, Alfaia, CM, Jerónimo, E, Prates, JAM, Santos-Silva, J and Bessa, RJB 2016. Effect of feeding lambs with a tanniferous shrub (rockrose) and a vegetable oil blend on fatty acid composition of meat lipids. Animal 12, 20612073.
Francisco, A, Dentinho, MT, Alves, SP, Portugal, PV, Fernandes, F, Sengo, S, Jerónimo, E, Oliveira, MA, Costa, P, Sequeira, A., Bessa, RJB and Santos-Silva, J 2015. Growth performance, carcass and meat quality of lambs supplemented with increasing levels of a tanniferous bush (Cistus ladanifer, L.) and vegetable oils. Meat Science 100, 275282.
Gebauer, SK, Chardigny, JM, Jakobsen, MU, Lamarche, B, Lock, AL, Proctor, SD and Baer, DJ 2011. Effects of ruminant trans fatty acids on cardiovascular disease and cancer: a comprehensive review of epidemiological, clinical, and mechanistic studies. Advances in Nutrition 2, 332354.
Grau, A, Guardiola, F, Boatella, J, Barroeta, A and Codony, R 2000. Measurement of 2-thiobarbituric acid values in dark chicken meat through derivative spectrophotometry: Influence of various parameters. Journal of Agriculture and Food Chemistry 48, 11551159.
Guerreiro, O, Dentinho, MT, Moreira, OC, Guerra, AR, Ramos, PAB, Bessa, RJB, Duarte, MF and Jerónimo, E 2016. Potential of Cistus ladanifer L. (rockrose) in small ruminant diets – effect of season and plant age on chemical composition, in vitro digestibility and antioxidant activity. Grass Forage Science 71, 437447.
Inserra, L, Priolo, A, Biondi, L, Lanza, M, Bognanno, M, Gravador, R and Luciano, G 2014. Dietary citrus pulp reduces lipid oxidation in lamb meat. Meat Science 96, 14891493.
ISO 1442 1997. Meat and meat products – determination of moisture content – reference method. International Organization for Standardization, Geneva, Switzerland.
ISO 2917 1999. Meat and meat products – measurement of pH – reference method. International Organization for Standardization, Geneva, Switzerland.
Jerónimo, E, Alves, SP, Dentinho, MTP, Martins, SV, Prates, JAM, Vasta, V, Santos-Silva, J and Bessa, RJB 2010. Effect of grape seed extract, Cistus ladanifer L., and vegetable oil supplementation on fatty acid composition of abomasal digesta and intramuscular fat of lambs. Journal of Agriculture and Food Chemistry 58, 1071010721.
Jerónimo, E, Alfaia, CMM, Alves, SP, Dentinho, MTP, Prates, JAM, Vasta, V, Santos-Silva, J and Bessa, RJB 2012. Effect of dietary grape seed extract and Cistus ladanifer L. in combination with vegetable oil supplementation on lamb meat quality. Meat Science 92, 841847.
Khiaosa-Ard, R, Bryner, SF, Scheeder, MRL, Wettstein, HR, Leiber, F, Kreuzer, M and Soliva, CR 2009. Evidence for the inhibition of the terminal step of ruminal α-linolenic acid biohydrogenation by condensed tannins. Journal of Dairy Science 92, 177188.
Lanza, M, Priolo, A, Biondi, L, Bella, M and Salem, HB 2001. Replacement of cereal grains by orange pulp and carob pulp in faba bean based diets fed to lambs: effects on growth performance and meat quality. Animal Research 50, 2130.
Lanza, M, Scerra, M, Bognanno, M, Buccioni, A, Cilione, C, Biondi, L, Priolo, A and Luciano, G 2015. Fatty acid metabolism in lambs fed citrus pulp. Journal of Animal Science 93, 31793188.
Lourenço, M, Ramos-Moralez, E and Wallace, RJ 2010. The role of microbes in rumen lipolysis and the biohydrogenation and their manipulation. Animal 4, 10081023.
Lund, MN, Heinonen, M, Baron, CP and Estevéz, M 2011. Protein oxidation in muscle foods: a review. Molecular Nutrition Food Research 55, 8395.
Maia, MRG, Bessa, RJB and Wallace, RJ 2009. Is the trans-10 shift that sometimes occurs in the ruminal biohydrogenation of linoleic acid caused by low pH or starch? A Rusitec study. In Ruminant physiology: digestion, metabolism, and effects of nutrition on reproduction and welfare, XIth international symposium on ruminant physiology, Clermont-Ferrand, France (ed. Y Chilliard, F Glasser, Y Faulconnier, F Bocquier, I Veissier and M Doreau), pp. 276–277, Wageningen Academic Publishers, Wageningen, The Netherlands.
Rodrigues, GH, Susin, I, Pires, AV, Mendes, CQ, Urano, FS and Castillo, CJC 2008. Citrus pulp in diets for feedlot lambs: carcass characteristics and meat quality. Revista Brasileira de Zootecnia 37, 18691875.
Santos-Silva, J, Dentinho, MT, Francisco, A, Portugal, AP, Belo, AT, Martins, APL, Alves, SP and Bessa, RJB 2016. Replacing cereals with dehydrated citrus pulp in a soybean oil supplemented diet increases vaccenic and rumenic acids in ewe milk. Journal of Dairy Science 99, 11731182.
Shingfield, KJ and Wallace, RJ 2014. Synthesis of conjugated linoleic acid in ruminants and humans. In Conjugated linoleic acids and conjugated vegetable oils (ed. B Sels and A Philippaerts), pp 165. The Royal Society of Chemistry, London, UK.
Sukhija, PS and Palmquist, DL 1988. Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. Journal of Agriculture and Food Chemistry 36, 12021206.
Tamate, H, Nagatani, T, Yoneya, S, Sakata, T and Miura, J 1973. High incidence of ruminal lesions and liver abscess in the beef associated with intensive fattening in Miyagi prefecture. Tohoku Journal of Agricultural Research 23, 184195.
Troegeler-Meynadier, A, Nicot, MC, Bayourthe, C, Moncoulon, R and Enjalbert, F 2003. Effects of pH and concentrations of linoleic and linolenic acids on extent and intermediates of ruminal biohydrogenation in vitro . Journal of Dairy Science 86, 40544063.
Vahmani, P, Rolland, DC, Gzyl, KE and Dugan, MER 2016. Non-conjugated cis/trans 18:2 in beef fat are mainly Δ-9 desaturation products of trans-18:1 isomers. Lipids 51, 14271433.
Vasta, V and Bessa, RJB 2012. Manipulating ruminal biohydrogenation by the use of plants bioactive compounds. In Dietary phytochemistry and microbes (ed. AK Patra), pp 263284. Springer, Dordrecht, The Netherlands.
Zened, A, Combes, S, Cauquil, L, Mariette, J, Klopp, C, Troegeler-Meynadier, A and Enjalbert, F 2013. Microbial ecology of the rumen evaluated by 454 GS FLX pyrosequencing is affected by starch and oil supplementation of diets. FEMS Microbiology Ecology 83, 504514.
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