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First cloned swamp buffalo produced from adult ear fibroblast cell

Published online by Cambridge University Press:  07 May 2014

K. Tasripoo ,
W. Suthikrai ,
S. Sophon ,
R. Jintana ,
W. Nualchuen ,
S. Usawang ,
A. Bintvihok ,
M. Techakumphu  and
K. Srisakwattana
K. Tasripoo
Affiliation:
Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
W. Suthikrai
Affiliation:
Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
S. Sophon
Affiliation:
Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
R. Jintana
Affiliation:
Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
W. Nualchuen
Affiliation:
Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
S. Usawang
Affiliation:
Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
A. Bintvihok
Affiliation:
Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
M. Techakumphu
Affiliation:
Department of Obstetrics Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
K. Srisakwattana*
Affiliation:
Research and Development Centre for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Bangkok 10330, Thailand
*
†E-mail: skitiya@chula.ac.th
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Abstract

The world’s first cloned swamp buffalo (Bubalus bubalis) derived from adult ear skin fibroblast has been reported. Donor fibroblast cells were produced from biopsies taken from adult male ear skin and in vitro matured oocytes obtained from a slaughterhouse were used as cytoplasts. A total of 39 blastocysts and 19 morulae fresh embryos were transferred into 12 recipient buffaloes. Progesterone assays indicated establishment of pregnancy in 10 of the 12 buffaloes (83.3%) after 45 days, with six animals still pregnant at 3 months. One recipient maintained pregnancy to term and naturally delivered a 40 kg male calf after 326 days of gestation. DNA analysis showed that the cloned calf was genetically identical to the donor cells. Genotype analyses, using 12 buffalo microsatellite markers, confirmed that the cloned calf was derived from the donor cell lines. In conclusion, the present study reports, for the first time, the establishment of pregnancy and birth of the first cloned Thai swamp buffalo derived from adult ear skin fibroblast cells.

Keywords

cloningear skin fibroblastsomatic cell nuclear transferswamp buffalo
Type
Full Paper
Information
animal , Volume 8 , Issue 7 , July 2014 , pp. 1139 - 1145
Copyright
© The Animal Consortium 2014 

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