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Oxidative stress status of highly prolific sows during gestation and lactation

Published online by Cambridge University Press:  03 June 2011

C. B. Berchieri-Ronchi ,
S. W. Kim ,
Y. Zhao ,
C. R. Correa ,
K.-J. Yeum  and
A. L. A. Ferreira
C. B. Berchieri-Ronchi
Affiliation:
Carotenoids and Health Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, 02111 MA, USA Botucatu Medical School, Sao Paulo State University, UNESP, 18618-970 Botucatu, Brazil
S. W. Kim*
Affiliation:
Department of Animal Science, North Carolina State University, Raleigh, 27695 NC, USA
Y. Zhao
Affiliation:
Department of Animal Science, North Carolina State University, Raleigh, 27695 NC, USA
C. R. Correa
Affiliation:
Carotenoids and Health Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, 02111 MA, USA Botucatu Medical School, Sao Paulo State University, UNESP, 18618-970 Botucatu, Brazil
K.-J. Yeum
Affiliation:
Carotenoids and Health Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, 02111 MA, USA
A. L. A. Ferreira
Affiliation:
Botucatu Medical School, Sao Paulo State University, UNESP, 18618-970 Botucatu, Brazil
*
†E-mail: sungwoo_kim@ncsu.edu
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Abstract

Elevated oxidative stress is reported to be associated with pregnancy complications in highly prolific sows. Oxidative DNA damage and the antioxidant status were determined in blood samples collected during the course of gestation and lactation in multiparous sows. Blood samples were drawn from sows (n = 5) on days 30, 60, 90 and 110 of gestation (G30, G60, G90 and G110, respectively), on day 3, 10 and 18 of lactation (L3, L10 and L18, respectively) and on day 5 of postweaning (W5). Lymphocytes were isolated from the fresh blood and cryopreserved in each time point. Lymphocyte DNA damage was analyzed by alkaline single-cell gel electrophoresis (comet assay) to determine the single- and double-strand brakes and endogenous antioxidant concentrations using an HPLC system with UV detection. The comet assay showed elevated (P < 0.05) DNA damage (between 38% and 47%) throughout the gestational and lactational periods than during early gestation (G30; 21%). Plasma retinol concentration was reduced (P < 0.05) at the end of gestation (G110) compared with G30. Plasma α-tocopherol concentrations also showed a similar trend as to retinol. This study indicates that there is an increased systemic oxidative stress during late gestation and lactation, which are not fully recovered until the weaning compared with the G30, and that antioxidant nutrients in circulation substantially reduced in the mother pig at G110.

Keywords

antioxidantDNA damageoxidative stresssows
Type
Full Paper
Information
animal , Volume 5 , Issue 11 , 26 September 2011 , pp. 1774 - 1779
Copyright
Copyright © The Animal Consortium 2011

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References

Agarwal, A, Saleh, RA, Bedaiwy, MA 2003. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertility and Sterility 79, 829–843.Google Scholar
Agarwal, A, Gupta, S, Sharma, RK 2005. Role of oxidative stress in female reproduction. Reproductive Biology and Endocrinology 3, 28.CrossRefGoogle ScholarPubMed
Casanueva, E, Viteri, FE 2003. Iron and oxidative stress in pregnancy. Journal of Nutrition 133 (suppl. 2), 1700S–1708S.Google Scholar
Chen, X, Scholl, TO 2005. Oxidative stress: changes in pregnancy and with gestational diabetes mellitus. Current Diabetes Reports 5, 282–288.Google Scholar
Collins, AR 2004. The comet assay for DNA damage and repair: principles, applications, and limitations. Molecular Biotechnology 26, 249–261.Google Scholar
Collins, A, Dusinska, M, Franklin, M, Somorovska, M, Petrovska, H, Duthie, S, Fillion, L, Panayiotidis, M, Raslova, K, Vaughan, N 1997. Comet assay in human biomonitoring studies: reliability, validation, and applications. Environmental and Molecular Mutagenesis 30, 139–146.3.0.CO;2-I>CrossRefGoogle ScholarPubMed
Duthie, SJ, Pirie, L, Jenkinson, AM, Narayanan, S 2002. Cryopreserved versus freshly isolated lymphocytes in human biomonitoring: endogenous and induced DNA damage, antioxidant status and repair capability. Mutagenesis 17, 211–214.CrossRefGoogle ScholarPubMed
Dusinska, M, Collins, A 2010. DNA oxidation, antioxidant effects and DNA repair measured with the comet assay. In Biomarkers for antioxidant defense and oxidative damage: principles and practical applications (ed. G Aldini, KJ Yeum, E Niki and RM Russell), p. 363.Wiley-Blackwell, IA, USA.Google Scholar
Ferreira, AL, Yeum, KJ, Liu, C, Smith, D, Krinsky, NI, Wang, XD, Russell, RM 2000. Tissue distribution of lycopene in ferrets and rats after lycopene supplementation. Journal of Nutrition 130, 1256–1260.Google Scholar
Gupta, S, Agarwal, A, Sharma, RK 2005. The role of placental oxidative stress and lipid peroxidation in preeclampsia. Obstetrical and Gynecological Survey 60, 807–816.Google Scholar
Heaton, PR, Ransley, R, Charlton, CJ, Mann, SJ, Stevenson, J, Smith, BH, Rawlings, JM, Harper, EJ 2002. Application of single-cell gel electrophoresis (comet) assay for assessing levels of DNA damage in canine and feline leukocytes. Journal of Nutrition 132 (suppl. 2), 1598S–1603S.Google Scholar
Hubel, CA 1999. Oxidative stress in the pathogenesis of preeclampsia. Proceedings of the Society for Experimental Biology and Medicine 222, 222–235.CrossRefGoogle ScholarPubMed
Jauniaux, E, Poston, L, Burton, GJ 2006. Placental-related diseases of pregnancy: involvement of oxidative stress and implications in human evolution. Human Reproduction Update 12, 747–755.CrossRefGoogle ScholarPubMed
Ji, F, Hurley, WL, Kim, SW 2006. Characterization of mammary gland development in pregnant gilts. Journal of Animal Science 84, 579–587.Google Scholar
Ji, F, Wu, G, Blanton, JR, Kim, SW 2005. Weight and compositional changes in pregnant gilts and its implication to nutrition. Journal of Animal Science 83, 366–375.CrossRefGoogle Scholar
Kim, SW, Hurley, WL, Wu, G, Ji, F 2009. Ideal amino acid balance for sows during gestation and lactation. Journal of Animal Science 87, E123–E132.CrossRefGoogle ScholarPubMed
Klemmensen, A, Tabor, A, Osterdal, ML, Knudsen, VK, Halldorsson, TI, Mikkelsen, TB, Olsen, SF 2009. Intake of vitamin C and E in pregnancy and risk of pre-eclampsia: prospective study among 57 346 women. BJOG 116, 964–974.Google Scholar
Marlin, DJ, Johnson, L, Kingston, DA, Smith, NC, Deaton, CM, Mann, S, Heaton, P, van Vugt, F, Saunders, K, Kydd, J, Harris, PA 2004. Application of the comet assay for investigation of oxidative DNA damage in equine peripheral blood mononuclear cells. Journal of Nutrition 134 (suppl. 2), 2133S–2140S.Google Scholar
Mateo, RD, Wu, G, Moon, HK, Carroll, JA, Kim, SW 2008. Effects of dietary arginine supplementation during gestation and lactation on the performance of lactating primiparous sows and nursing piglets. Journal of Animal Science 86, 827–835.Google Scholar
Mateo, RD, Carroll, JA, Hyun, Y, Smith, S, Kim, SW 2009. Effect of dietary supplementation of omega-3 fatty acids and high protein on reproductive outcome of primiparous sows for two parities. Journal of Animal Science 87, 948–959.Google Scholar
Mateo, RD, Wu, G, Bazer, FW, Park, JC, Shinzato, I, Kim, SW 2007. Dietary L-arginine supplementation improves pregnancy outcome in gilts. Journal of Nutrition 137, 652–656.CrossRefGoogle Scholar
McPherson, RL, Ji, F, Wu, G, Kim, SW 2004. Fetal growth and compositional changes of fetal tissues in the pigs. Journal of Animal Science 82, 2534–2540.Google Scholar
Mueller, A, Koebnick, C, Binder, H, Hoffmann, I, Schild, RL, Beckmann, MW, Dittrich, R 2005. Placental defence is considered sufficient to control lipid peroxidation in pregnancy. Medical Hypotheses 64, 553–557.Google Scholar
Myatt, L, Cui, X 2004. Oxidative stress in the placenta. Histochemistry and Cell Biology 122, 369–382.Google Scholar
National research Council (NRC) 1998. Nutritional requirements of pigs, 10th edition. National Academy Press, Washington, DC.Google Scholar
Pinelli-Saavedra, A 2003. Vitamin E in immunity and reproductive performance in pigs. Reproduction Nutrition Development 43, 397–408.Google Scholar
Pinelli-Saavedra, A, Scaife, JR 2005. Pre- and postnatal transfer of vitamin E and C to piglets in sows supplemented with vitamin E and vitamin C. Livestock Production Science 97, 231–240.Google Scholar
Pinelli-Saavedra, A, Calderon De La Barca, AM, Hernandez, J, Valenzuela, R, Scaife, JR 2008. Effect of supplementing sows’ feed with alpha-tocopherol acetate and vitamin C on transfer of alpha-tocopherol to piglet tissues, colostrum, and milk: aspects of immune status of piglets. Research in Veterinary Science 85, 92–100.Google Scholar
Prater, MR, Laudermilch, CL, Liang, C, Holladay, SD 2008. Placental oxidative stress alters expression of murine osteogenic genes and impairs fetal skeletal formation. Placenta 29, 802–808.Google Scholar
Reyes, MR, Sifuentes-Alvarez, A, Lazalde, B 2006. Estrogens are potentially the only steroids with an antioxidant role in pregnancy: in vitro evidence. Acta Obstetricia et Gynecologica Scandinavica 85, 1090–1093.CrossRefGoogle ScholarPubMed
Ruder, EH, Hartman, TJ, Goldman, MB 2009. Impact of oxidative stress on female fertility. Current Opinion in Obstetrics and Gynecology 21, 219–222.Google Scholar
Serdar, Z, Gur, E, Colakoethullary, M, Develioethlu, O, Sarandol, E 2003. Lipid and protein oxidation and antioxidant function in women with mild and severe preeclampsia. Archives of Gynecology and Obstetrics 268, 19–25.CrossRefGoogle ScholarPubMed
Sugino, N, Takiguchi, S, Umekawa, T, Heazell, A, Caniggia, I 2007. Oxidative stress and pregnancy outcome: a workshop report. Placenta 28 (suppl. A), S48–S50.CrossRefGoogle ScholarPubMed
Tremellen, K 2008. Oxidative stress and male infertility-a clinical perspective. Human Reproduction Update 14, 243–258.CrossRefGoogle ScholarPubMed
Wuryastuti, H, Stowe, HD, Bull, RW, Miller, ER 1993. Effects of vitamin E and selenium on immune responses of peripheral blood, colostrum, and milk leukocytes of sows. Journal of Animal Science 71, 2464–2472.CrossRefGoogle ScholarPubMed
Yeum, KJ, Booth, SL, Sadowski, JA, Liu, C, Tang, G, Krinsky, NI, Russell, RM 1996. Human plasma carotenoid response to the ingestion of controlled diets high in fruits and vegetables. American Journal of Clinical Nutrition 64, 594–602.CrossRefGoogle Scholar
Zhao, X, Aldini, G, Johnson, EJ, Rasmussen, H, Kraemer, K, Woolf, H, Musaeus, N, Krinsky, NI, Russell, RM, Yeum, KJ 2006. Modification of lymphocyte DNA damage by carotenoid supplementation in postmenopausal women. American Journal of Clinical Nutrition 83, 163–169.CrossRefGoogle ScholarPubMed