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Skatole metabolites in urine as a biological marker of pigs with enhanced hepatic metabolism

Published online by Cambridge University Press:  15 April 2016

C. Brunius ,
J. K. Vidanarachchi ,
J. Tomankova ,
K. Lundström ,
K. Andersson  and
G. Zamaratskaia
C. Brunius*
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences, BioCenter, PO Box 7051, SE-750 07 Uppsala, Sweden
J. K. Vidanarachchi
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences, BioCenter, PO Box 7051, SE-750 07 Uppsala, Sweden Department of Animal Science, University of Peradeniya, 20400 Peradeniya, Sri Lanka
J. Tomankova
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences, BioCenter, PO Box 7051, SE-750 07 Uppsala, Sweden
K. Lundström
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences, BioCenter, PO Box 7051, SE-750 07 Uppsala, Sweden
K. Andersson
Affiliation:
Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, PO Box 7024, SE-750 07 Uppsala, Sweden
G. Zamaratskaia
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences, BioCenter, PO Box 7051, SE-750 07 Uppsala, Sweden
*
†E-mail: carl.brunius@slu.se
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Abstract

Boar taint is a quality defect in meat, related to accumulation of skatole and androstenone in male pigs. The levels of skatole and its main metabolites in plasma and urine samples were measured with a validated liquid chromatography-MS method and related to activity of hepatic cytochrome P450 (CYP450) in order to identify ‘fast metabolizing’ pigs. Urine (n=46), blood (n=12), liver (n=25) and adipose tissue (n=46) were sampled from a total of 46 entire male pigs. Skatole levels in fat were negatively correlated to CYP2E1 activity and positively to 3-hydroxy-3-methyloxindole (HMOI), indole-3-carboxylic acid (ICA) and 2-aminoacetophenone in urine. HMOI and ICA levels in urine were the best predictors of high skatole levels in fat. In summary, the present study provided further evidence for the key role of CYP2E1 in skatole metabolism and suggested that measurement of HMOI and/or ICA in urine might provide information about skatole levels in live pigs.

Keywords

pigskatole3-hydroxy-3-methyloxindole (HMOI)indole-3-carboxylic acid (ICA)cytochrome P450 (CYP450)
Type
Research Article
Information
animal , Volume 10 , Issue 10 , October 2016 , pp. 1734 - 1740
Copyright
© The Animal Consortium 2016 

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