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Different effects of casein and soyabean protein on gastric emptying of protein and small intestinal transit after spontaneous feeding of diets in rats

Published online by Cambridge University Press:  09 March 2007

Hiroshi Hara ,
Hiroyuki Nishikawa  and
Shuhachi Kiriyama
Hiroshi Hara
Affiliation:
Laboratory of Nutritional Biochemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan
Hiroyuki Nishikawa
Affiliation:
Laboratory of Nutritional Biochemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan
Shuhachi Kiriyama
Affiliation:
Laboratory of Nutritional Biochemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan
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Abstract

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The effects of dietary casein and soyabean-protein isolate (SPI) on gastric emptying and small intestinal transit were observed in rats fed on an 80 g casein or 80 g SPI/kg diet. After a 24 h fast, rats were given 2 g of both the test diets containing 10 g guanidinated casein/kg diet as a marker protein. The amounts of the marker protein remaining in the stomach of the rats fed on the casein or SPI diet were similar and decreased to about 50% after 20min. The emptying rate then slowed, especially in the casein group, so that the amount leaving the stomach after 1 h in the SPI group was slightly higher (P < 0.05). The small intestinal transit of chyme was estimated by a bolus injection of colloidal carbon suspension or of colloidal carbon and 3H-labelled polyethylene glycol through an implanted duodenal catheter 6 min before death. The average value of transit at 12, 20, 40 and 60 min after feeding of SPI diet was about 25% faster than that after casein diet. The transit velocity of the SPI group was also faster than that of the non-protein group 40 min after feeding. These findings reveal that SPI enhances the small intestinal transit of the liquid phase of chyme. There was no correlation between the gastric emptying of homoarginine and small intestinal transit. This result suggests that the small intestinal transit of lumen contents is controlled by the dietary protein regardless of the gastric emptying of protein.

Keywords

Gastric emptyingSmall intestinal transitDietary protein
Type
Diet and Intestinal Function
Information
British Journal of Nutrition , Volume 68 , Issue 1 , July 1992 , pp. 59 - 66
Copyright
Copyright © The Nutrition Society 1992

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