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Effects of Si-Jun-Zi decoction polysaccharides on cell migration and gene expression in wounded rat intestinal epithelial cells

Published online by Cambridge University Press:  08 March 2007

L. Liu ,
L. Han ,
Daisy Y. L. Wong ,
Patrick Y. K. Yue ,
W. Y. Ha ,
Y. H. Hu ,
P. X. Wang  and
Ricky N. S. Wong
L. Liu
Affiliation:
Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong
L. Han
Affiliation:
Immunology Laboratory, Guangzhou University of Traditional Chinese Medicine, Guangzhou, People's Republic of China
Daisy Y. L. Wong
Affiliation:
Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong
Patrick Y. K. Yue
Affiliation:
Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong
W. Y. Ha
Affiliation:
Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong
Y. H. Hu
Affiliation:
Department of Biology, Science Faculty, Hong Kong Baptist University, Kowloon Tong, Hong Kong
P. X. Wang
Affiliation:
Immunology Laboratory, Guangzhou University of Traditional Chinese Medicine, Guangzhou, People's Republic of China
Ricky N. S. Wong*
Affiliation:
Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong Department of Biology, Science Faculty, Hong Kong Baptist University, Kowloon Tong, Hong Kong
*
*Corresponding author: Dr Ricky N. S. Wong, fax +852 3411 5995, email rnswong@hkbu.edu.hk
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Abstract

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Si-Jun-Zi decoction (SJZD), a traditional Chinese herbal prescription, has been used clinically for treating patients with disorders of the digestive system. Previous studies indicated that the polysaccharides of SJZD (SJZPS) are the active components contributing towards its pharmacological effects in improving gastrointestinal function and immunity. However, the protective and restitutive effects on intestinal epithelial cells remain unknown. In the present study, SJZPS were first extracted and chemically characterized. Then their stimulatory and restitutive effects on intestinal epithelial cells (IEC-6 cells) were elicited by different in vitro models including migration of wounded IEC-6 cells and cell proliferation. Results indicated that SJZPS not only protects the cells against the harmful impairment of indomethacin but also enhances re-epithelialization of a wounded monolayer at an optimal dose of 100 μg/ml at 24 h incubation. To elucidate the modulatory effect of SJZPS on wounded IEC-6 cells at the molecular level, an oligonucleotide microarray was employed to study differential gene expression of SJZPS-treated IEC-6 cells and the candidate genes were validated by RT-PCR. There was increased expression of genes coding for ion channels and transporters, which are critical to cell migration and restoration of wounded intestinal cells, suggesting a possible mechanism for re-epithelialization. In conclusion, our data show for the first time that SJZPS can enhance intestinal restitution and protect against indomethacin-induced damage of intestinal epithelial cells. These findings provide new insight into the mechanism of action of a traditional Chinese herbal prescription, SJZD, in intestinal wound restitution.

Keywords

Si-Jun-Zi decoctionPolysaccharidesIEC-6 cellMicroarray
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 1 , January 2005 , pp. 21 - 29
Copyright
Copyright © The Nutrition Society 2005

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