Skip to main content
    • Aa
    • Aa
  • Get access
    Check if you have access via personal or institutional login
  • Cited by 53
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Barbosa, Priscila Oliveira Pala, Daniela Silva, Carla Teixeira de Souza, Melina Oliveira do Amaral, Joana Ferreira Vieira, Renata Adrielle Lima Folly, Gilce Andrezza de Freitas Volp, Ana Carolina Pinheiro and de Freitas, Renata Nascimento 2016. Açai (Euterpe oleracea Mart.) pulp dietary intake improves cellular antioxidant enzymes and biomarkers of serum in healthy women. Nutrition, Vol. 32, Issue. 6, p. 674.

    Cooperstone, J.L. and Schwartz, S.J. 2016. Handbook on Natural Pigments in Food and Beverages.

    Peluso, Ilaria and Raguzzini, Anna 2016. Salivary and Urinary Total Antioxidant Capacity as Biomarkers of Oxidative Stress in Humans. Pathology Research International, Vol. 2016, p. 1.

    Periago, María and García-Alonso, Francisco-Javier 2016. Handbook of Functional Beverages and Human Health.

    Stedile, Natalia Canuto, Raquel de Col, Camila Dallavechia de Sene, Juliane Souza Stolfo, Adriana Wisintainer, Gabrielle Nunes de Souza Henriques, João Antonio Pêgas and Salvador, Mirian 2016. Dietary total antioxidant capacity is associated with plasmatic antioxidant capacity, nutrient intake and lipid and DNA damage in healthy women. International Journal of Food Sciences and Nutrition, Vol. 67, Issue. 4, p. 479.

    Flynn, Tanya J Cadzow, Murray Dalbeth, Nicola Jones, Peter B Stamp, Lisa K Hindmarsh, Jennie Harré Todd, Alwyn S Walker, Robert J Topless, Ruth and Merriman, Tony R 2015. Positive association of tomato consumption with serum urate: support for tomato consumption as an anecdotal trigger of gout flares. BMC Musculoskeletal Disorders, Vol. 16, Issue. 1,

    Ghavipour, Mahsa Sotoudeh, Gity and Ghorbani, Mohammad 2015. Tomato juice consumption improves blood antioxidative biomarkers in overweight and obese females. Clinical Nutrition, Vol. 34, Issue. 5, p. 805.

    Kim, Min-Ju Kim, Jung-In Kang, Min-Jung Kwon, Bokyeong Jun, Jung-Guy Choi, Ji-Hye and Kim, Myo-Jeong 2015. Quality evaluation of fresh tomato juices prepared using high-speed centrifugal and low-speed masticating household juicers. Food Science and Biotechnology, Vol. 24, Issue. 1, p. 61.

    Kuchay, Raja A.H. Bhatia, Alka Mahmood, Akhtar Anwar, Mumtaz and Mahmood, Safrun 2015. Brush border enzyme activity and expression of apoptotic marker genes in lycopene fed rats with 5-Fu induced gastrointestinal mucositis. Nutrition and Aging, Vol. 3, Issue. 1, p. 1.

    Lee, Li-Chen Wei, Li Huang, Wen-Ching Hsu, Yi-Ju Chen, Yi-Ming and Huang, Chi-Chang 2015. Hypolipidemic Effect of Tomato Juice in Hamsters in High Cholesterol Diet-Induced Hyperlipidemia. Nutrients, Vol. 7, Issue. 12, p. 10525.

    Li, Yu-Fen Chang, Ya-Yuan Huang, Hui-Chi Wu, Yi-Chen Yang, Mei-Due and Chao, Pei-Min 2015. Tomato juice supplementation in young women reduces inflammatory adipokine levels independently of body fat reduction. Nutrition, Vol. 31, Issue. 5, p. 691.

    Martín-Pozuelo, Gala Navarro-González, Inmaculada González-Barrio, Rocío Santaella, Marina García-Alonso, Javier Hidalgo, Nieves Gómez-Gallego, Carlos Ros, Gaspar and Periago, María Jesús 2015. The effect of tomato juice supplementation on biomarkers and gene expression related to lipid metabolism in rats with induced hepatic steatosis. European Journal of Nutrition, Vol. 54, Issue. 6, p. 933.

    Shidfar, Farzad and Arjomand, Gol-Naz 2015. Glucose Intake and Utilization in Pre-Diabetes and Diabetes.

    Tonin, Fernanda Steimbach, Laiza Wiens, Astrid Perlin, Cássio and Pontarolo, Roberto 2015. Impact of Natural Juice Consumption on Plasma Antioxidant Status: A Systematic Review and Meta-Analysis. Molecules, Vol. 20, Issue. 12, p. 22146.

    Joseph, Serlene Chatli, Manish K. Biswas, Ashim K. and Sahoo, Jhari 2014. Oxidative stability of pork emulsion containing tomato products and pink guava pulp during refrigerated aerobic storage. Journal of Food Science and Technology, Vol. 51, Issue. 11, p. 3208.

    Petrosino, Teresa and Serafini, Mauro 2014. Antioxidant Modulation of F2-Isoprostanes in Humans: A Systematic Review. Critical Reviews in Food Science and Nutrition, Vol. 54, Issue. 9, p. 1202.

    Raiola, Assunta Rigano, Maria Manuela Calafiore, Roberta Frusciante, Luigi and Barone, Amalia 2014. Enhancing the Health-Promoting Effects of Tomato Fruit for Biofortified Food. Mediators of Inflammation, Vol. 2014, p. 1.

    Reyes-De-Corcuera, José I. Goodrich-Schneider, Renée M. Barringer, Sheryl A. and Landeros-Urbina, Miguel A. 2014. Food Processing.

    Riso, Patrizia Vendrame, Stefano Del Bo', Cristian Martini, Daniela Martinetti, Antonia Seregni, Ettore Visioli, Francesco Parolini, Marina and Porrini, Marisa 2014. Effect of 10-day broccoli consumption on inflammatory status of young healthy smokers. International Journal of Food Sciences and Nutrition, Vol. 65, Issue. 1, p. 106.

    Tsitsimpikou, Christina Tsarouhas, Konstantinos Kioukia-Fougia, Nassia Skondra, Christina Fragkiadaki, Persefoni Papalexis, Peter Stamatopoulos, Panagiotis Kaplanis, Ioannis Hayes, A. Wallace Tsatsakis, Aristidis and Rentoukas, Elias 2014. Dietary supplementation with tomato-juice in patients with metabolic syndrome: A suggestion to alleviate detrimental clinical factors. Food and Chemical Toxicology, Vol. 74, p. 9.


Influence of lycopene and vitamin C from tomato juice on biomarkers of oxidative stress and inflammation

  • Karin Jacob (a1), María J. Periago (a1), Volker Böhm (a2) and Gaspar Ros Berruezo (a1)
  • DOI:
  • Published online: 01 January 2008

A human study was carried out to investigate whether tomato juice, rich in natural lycopene and fortified with vitamin C, is able to reduce several biomarkers of oxidative stress and inflammation and whether the effect can be attributed to lycopene, vitamin C or any other micronutrient. Following a 2-week depletion phase, volunteers were assigned randomly to ingest either tomato juice with (LC) or without (L) vitamin C fortification for 2 weeks (daily dose 20·6 mg lycopene and 45·5/435 mg vitamin C). Plasma and urine were analysed for carotenoids and vitamin C, lipid status, antioxidant capacity, thiobarbituric acid reactive substances (TBARS) and 8-epi-PGF, protein carbonyls, cytokines IL-1β and TNFα and C-reactive protein (CRP). The consumption of tomato juice led to a reduction in total cholesterol levels (L: 157·6 v. 153·2 mg/dl, P = 0·008; LC: 153·4 v. 147·4 mg/dl, P = 0·002) and that of CRP (L: 315·6 v. 262·3 μg/l, P = 0·017; LC: 319·2 v. 247·1 μg/l, P = 0·001) in both groups. The vitamin C-fortified juice slightly raised the antioxidant capacity in urine and decreased TBARS in plasma and urine. All other markers were affected to a lesser extent or remained unchanged. Cholesterol reduction was correlated with lycopene uptake (P = 0·003), whereas the other effects could not be related with particular micronutrients. Any beneficial effects of tomato consumption for human health cannot be attributed only to lycopene and, as the additional supplementation with ascorbic acid indicates, a variety of antioxidants might be needed to optimize protection against chronic diseases.

Corresponding author
*Corresponding author: Dr M.J. Periago, fax +34 968 361447, email
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

1AV Rao & LG Rao (2007) Carotenoids and human health. Pharmacol Res 55, 207216.

2S Agarwal & AV Rao (1998) Tomato lycopene and low density lipoprotein oxidation: a human dietary intervention study. Lipids 33, 981984.

4N Sakuma , T Hibino , T Sato , N Ohte , S Akita , N Tamai , K Sasai , T Yoshimata & T Fujinami (1997) Levels of thiobarbituric acid-reactive substance in plasma from coronary artery disease patients. Clin Biochem 30, 505507.

5MF Walter , RF Jacob , B Jeffers , MM Ghadanfar , GM Preston , J Buch & RP Mason (2004) Serum levels of thiobarbituric acid reactive substances predict cardiovascular events in patients with stable coronary artery disease: A longitudinal analysis of the PREVENT study. J Am Coll Cardio 44, 19962002.

6A Salvi , PA Carrupt , JP Tillement & B Testa (2001) Structural damage to proteins caused by free radicals: assessment, protection by antioxidants, and influence of protein binding. Biochem Pharmacol 61, 12371242.

7I Dalle-Donne , D Giustarini , R Colombo , R Rossi & A Milzani (2003) Protein carbonylation in human diseases. Trends Mol Med 9, 169176.

10PM Ridker , CH Hennekens , JE Buring & N Rifai (2000) C-reactive protein and other markers of inflammation in the prediction of cardiovascular disease in women. N Engl J Med 342, 836842.

12C Sánchez-Moreno , MP Cano , B De Ancos , L Plaza , B Olmedilla , F Granado & A Martin (2006) Mediterranean vegetable soup consumption increases plasma vitamin C and decreases F2-isoprostanes, prostaglandin E2 and monocyte chemotactic protein-1 in healthy humans. J Nutr Biochem 17, 183189.

15Y Klopotek , K Otto & V Böhm (2005) Processing strawberries to different products alters contents of vitamin C, total phenolics, total anthocyanins and antioxidant capacity. J Agric Food Chem 53, 56405646.

17V Böhm (2001) Use of column temperature to optimise carotenoid isomer separation by C30 high performance liquid chromatography. J Sep Sci 24, 955959.

18C Seybold , K Fröhlich , R Bitsch , K Otto & V Böhm (2004) Changes in contents of carotenoids and vitamin E during tomato processing. J Agric Food Chem 52, 70057010.

19S Gahler , K Otto & V Böhm (2003) Alterations of vitamin C, total phenolics, and antioxidant capacity as affected by processing tomatoes to different products. J Agric Food Chem 51, 79627968.

22AW Wahlefeld (1974) Triglycerides determination after enzymatic hydrolysis. In Method of Enzymatic Analysis, pp. 18311833 [AW Wahlefeld and HU Bergmeyer , editors]. New York: Academic Press.

23R Re , N Pellegrini , A Proteggente , A Pannala , M Yang & C Rice-Evans (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26, 12311237.

24K Schlesier , M Harwat , V Böhm & R Bitsch (2002) Assessment of antioxidant activity by using different in vitro methods. Free Radic Res 36, 177187.

25GF Domagk & HH Schlicke (1968) A colourimetric method using uricase and peroxidase for the determination of uric acid. Anal Biochem 22, 219224.

27RL Levine , D Garland , CN Oliver , A Amici , I Climent , AG Lenz , BW Ahn , S Shaltiel & ER Stadtman (1990) Determination of carbonyl content in oxidatively modified proteins. Meths Enzymol 186, 464478.

28AZ Reznick & L Packer (1994) Oxidative damage to proteins: Spectroscopic method for carbonyl assay. Meth Enzymiol 233, 357363.

29JA Buege & SD Aust (1978) Microsomal lipid peroxidation. Meths Enzymol. 52, 302310.

30J Nourooz-Zadeh , J Tajaddini-Sarmadi & SP Wolf (1994) Measurement of plasma hydroperoxide concentrations by the ferrous oxidation-xylenol orange assay in conjunction with triphenilphosphine. Anal Biochem 220, 403409.

31B Beutler , D Greenwald , JD Hulmes , . (1985) Identity of tumor necrosis factor and the macrophage-secreted factor cachectin. Nature 316, 552554.

32J Grassi , CJ Roberge , Y Frobert , . (1991) Determination of ILα, IL1βand IL2 in biological media using specific enzyme immunometric assays. Immunol Rev 119, 125142.

34LJ Roberts II & JD Morrow (2000) Measurement of F2-isoprostanes as an index of oxidative stress in vivo. Free Radic Biol Med 28, 505513.

35S Georgé , P Bart , P Alter & MJ Amiot (2005) Rapid determination of polyphenols and vitamin C in plant-derived products. J Agric Food Chem 53, 13701373.

40AV Rao & H Shen (2002) Effect of low dose lycopene intake on lycopene bioavailability and oxidative stress. Nutr Res 22, 11251131.

42RB Van Breemen , X Xu , MA Viana , L Chen , M Stacewicz-Sapuntzakis , C Duncan , PE Bowen & R Sharifi (2002) Liquid chromatography-mass spectrometry of cis- and all-trans-lycopene in human serum and prostate tissue after dietary supplementation with tomato sauce. J Agric Food Chem 50, 22142219.

46B Fuhrman , A Elis & M Aviram (1997) Hypocholesterolemic effect of lycopene and β-carotene is related to suppression of cholesterol synthesis and augmentation of LDL receptor activity in macrophages. Biochem Biophys Res Comm 233, 658662.

47JK Willcox , GL Catignani & S Lazarus (2003) Tomatoes and cardiovascular health. Crit Rev Food Sci Nutr 43, 118.

48N Pellegrini , P Riso & M Porrini (2000) Tomato consumption does not affect the total antioxidant capacity of plasma. Nutr 16, 268271.

49B Kirschbaum (2001) Total urine antioxidant capacity. Clin Chim Acta 305, 167173.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *