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Macauba (Acrocomia aculeata) pulp oil has the potential to enhance the intestinal barrier morphology, goblet cell proliferation and gut microbiota composition in mice fed a high-fat diet

Published online by Cambridge University Press:  13 November 2023

Cíntia Tomaz Sant’ Ana ,
Thaísa Agrizzi Verediano ,
Mariana Grancieri ,
Renata Celi Lopes Toledo ,
Neuza Maria Brunoro Costa ,
Hércia Stampini Duarte Martino  and
Frederico Augusto Ribeiro de Barros Open the ORCID record for Frederico Augusto Ribeiro de Barros [Opens in a new window]
Cíntia Tomaz Sant’ Ana
Affiliation:
Department of Food Technology, Federal University of Viçosa, Viçosa, MG 36570-000, Brazil
Thaísa Agrizzi Verediano
Affiliation:
Department of Nutrition and Health, Federal University of Viçosa, Viçosa, MG, Brazil
Mariana Grancieri
Affiliation:
Department of Nutrition and Health, Federal University of Viçosa, Viçosa, MG, Brazil
Renata Celi Lopes Toledo
Affiliation:
Department of Nutrition and Health, Federal University of Viçosa, Viçosa, MG, Brazil
Neuza Maria Brunoro Costa
Affiliation:
Department of Pharmacy and Nutrition, Federal University of Espírito Santo, Alegre, ES, Brazil
Hércia Stampini Duarte Martino
Affiliation:
Department of Nutrition and Health, Federal University of Viçosa, Viçosa, MG, Brazil
Frederico Augusto Ribeiro de Barros*
Affiliation:
Department of Food Technology, Federal University of Viçosa, Viçosa, MG 36570-000, Brazil
*
*Corresponding author: Frederico Augusto Ribeiro de Barros, email fredbarros@ufv.br
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Abstract

Macauba (Acrocomia aculeata) is a palm tree native from Brazil, whose pulp is rich in oil that has a high content of oleic acid and carotenoids. Macauba pulp oil can bring health benefits due to its bioactive compounds; however, its effects on gut health are unknown. Thus, the objective of this study was to evaluate the effect of macauba pulp oil on the intestinal health in mice fed a high-fat (HF) diet. Male C57BL1/6 mice were randomly divided into three groups (10 animals/group): control diet, HF diet and HF diet with 4 % of macauba pulp oil (HFM). Concentration of short-chain fatty acids (SCFA), faecal pH and histomorphometric analysis of the colon were performed. Content of colon samples was used on microbiome analysis using 16S rRNA amplicon sequencing. Animals from the HFM group had higher butyric acid content and goblet cells number, greater circular and longitudinal muscle layer and higher α-diversity compared with the HF group. Moreover, consumption of MPO reduced Desulfobacterota phylum, Ruminococcaceae, Oscillospiraceae, Prevotellaceae, Bifidobacteriaceae family, Faecalibacterium, Prevotella, Ruminococcus and Enterorhabdus genus. Therefore, macauba pulp oil was able to modulate the gut microbiota and enhance intestinal barrier morphology, showing preventive effects on gut dysbiosis in mice fed a HF diet.

Keywords

HPLCButyrateOleic acidCarotenoidsGut microbiotaGoblet cell
Type
Research Article
Information
British Journal of Nutrition , Volume 131 , Issue 6 , 28 March 2024 , pp. 987 - 996
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Copyright
© The Author(s), 2023. Published by Cambridge University Press on behalf of The Nutrition Society

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