Skip to main content
    • Aa
    • Aa
  • Access
  • Cited by 57
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Arnold, Jason W. Roach, Jeffrey and Azcarate-Peril, M. Andrea 2016. Emerging Technologies for Gut Microbiome Research. Trends in Microbiology,

    Moloney, Rachel D. Johnson, Anthony C. O'Mahony, Siobhain M. Dinan, Timothy G. Greenwood-Van Meerveld, Beverley and Cryan, John F. 2016. Stress and the Microbiota-Gut-Brain Axis in Visceral Pain: Relevance to Irritable Bowel Syndrome. CNS Neuroscience & Therapeutics, Vol. 22, Issue. 2, p. 102.

    Petersen, Beate Egert, Sarah Bosy-Westphal, Anja Müller, Manfred J. Wolffram, Siegfried Hubbermann, Eva M. Rimbach, Gerald and Schwarz, Karin 2016. Bioavailability of quercetin in humans and the influence of food matrix comparing quercetin capsules and different apple sources. Food Research International,

    Maukonen, Johanna and Saarela, Maria 2015. Human gut microbiota: does diet matter?. Proceedings of the Nutrition Society, Vol. 74, Issue. 01, p. 23.

    Neuman, Manuela G. French, Samuel W. French, Barbara A. Seitz, Helmut K. Cohen, Lawrence B. Mueller, Sebastian Osna, Natalia A. Kharbanda, Kusum K. Seth, Devanshi Bautista, Abraham Thompson, Kyle J. McKillop, Iain H. Kirpich, Irina A. McClain, Craig J. Bataller, Ramon Nanau, Radu M. Voiculescu, Mihai Opris, Mihai Shen, Hong Tillman, Brittany Li, Jun Liu, Hui Thomes, Paul G. Ganesan, Murali and Malnick, Steve 2014. Alcoholic and non-alcoholic steatohepatitis. Experimental and Molecular Pathology, Vol. 97, Issue. 3, p. 492.

    Taddei, Carla R. Oliveira, Fernanda F. Duarte, Rubens T. D. Talarico, Silvia T. Takagi, Elizabeth H. Ramos Carvalho, Isabel I. Gomes, Filumena M. S. Brandt, Kátia and Martinez, Marina B. 2014. High Abundance of Escherichia During the Establishment of Fecal Microbiota in Brazilian Children. Microbial Ecology, Vol. 67, Issue. 3, p. 624.

    Ariefdjohan, Merlin W. Brown-Esters, Onikia N. and Savaiano, Dennis A. 2013. Nutrition in the Prevention and Treatment of Disease.

    Umu, Özgün Candan Onarman Oostindjer, Marije Pope, Phillip B. Svihus, Birger Egelandsdal, Bjørg Nes, Ingolf F. and Diep, Dzung B. 2013. Potential applications of gut microbiota to control human physiology. Antonie van Leeuwenhoek, Vol. 104, Issue. 5, p. 609.

    Hashad, Mohamed Mohie Eldin Atta, Mohamed Elabbady, Ahmed Elfiky, Souzan Khattab, Amr and Kotb, Ahmed 2012. Safety of no bowel preparation before ileal urinary diversion. BJU International, Vol. 110, Issue. 11c, p. E1109.

    Loo, Jan Van 2012. Prebiotics: Development & Application.

    Rosendale, Douglas I. Blatchford, Paul A. Sims, Ian M. Parkar, Shanthi G. Carnachan, Susan M. Hedderley, Duncan and Ansell, Juliet 2012. Characterizing Kiwifruit Carbohydrate Utilizationin vitroand its Consequences for Human Faecal Microbiota. Journal of Proteome Research, p. 121026145750009.

    Tuohy, Kieran M. and McCartney, Anne L. 2012. Prebiotics: Development & Application.

    Jia, J. Frantz, N. Khoo, C. Gibson, G.R. Rastall, R.A. and McCartney, A.L. 2011. Investigation of the faecal microbiota of geriatric cats. Letters in Applied Microbiology, Vol. 53, Issue. 3, p. 288.

    Mai, Volker Ukhanova, Maria and Baer, David J. 2010. Understanding the Extent and Sources of Variation in Gut Microbiota Studies; a Prerequisite for Establishing Associations with Disease. Diversity, Vol. 2, Issue. 9, p. 1085.

    Millet, S. Van Oeckel, M. J. Aluwé, M. Delezie, E. and De Brabander, D. L. 2010. Prediction of In Vivo Short-Chain Fatty Acid Production in Hindgut Fermenting Mammals: Problems and Pitfalls. Critical Reviews in Food Science and Nutrition, Vol. 50, Issue. 7, p. 605.

    Roberfroid, Marcel Gibson, Glenn R. Hoyles, Lesley McCartney, Anne L. Rastall, Robert Rowland, Ian Wolvers, Danielle Watzl, Bernhard Szajewska, Hania Stahl, Bernd Guarner, Francisco Respondek, Frederique Whelan, Kevin Coxam, Veronique Davicco, Marie-Jeanne Léotoing, Laurent Wittrant, Yohann Delzenne, Nathalie M. Cani, Patrice D. Neyrinck, Audrey M. and Meheust, Agnes 2010. Prebiotic effects: metabolic and health benefits. British Journal of Nutrition, Vol. 104, Issue. S2, p. S1.

    Sturtzel, Baerbel Dietrich, A. Wagner, K. -H. Gisinger, C. and Elmadfa, I. 2010. The status of vitamins B6, B12, folate, and of homocysteine in geriatric home residents receiving laxatives or dietary fiber. The journal of nutrition, health & aging, Vol. 14, Issue. 3, p. 219.

    Edwards, C.A. and Garcia, A.L. 2009. Handbook of Hydrocolloids.

    Flint, H.J. Duncan, S.H. and Louis, P. 2009. Designing Functional Foods.

    Forssten, Sofia Lahtinen, Sampo and Ouwehand, Arthur C. 2009. Probiotics for the elderly. Food Science & Technology Bulletin: Functional Foods, Vol. 6, Issue. 3, p. 31.


Molecular biological methods for studying the gut microbiota: the EU human gut flora project

  • M. Blaut (a1), M.D. Collins (a2), G. W. Welling (a3), J. Doré (a4), J. van Loo (a5) and W. de Vos (a6)
  • DOI:
  • Published online: 01 March 2007

Seven European laboratories co-operated in a joint project (FAIR CT97-3035) to develop, refine and apply molecular methods towards facilitating elucidation of the complex composition of the human intestinal microflora and to devise robust methodologies for monitoring the gut flora in response to diet. An extensive database of 16S rRNA sequences for tracking intestinal bacteria was generated by sequencing the 16S rRNA genes of new faecal isolates and of clones obtained by amplification with polymerase chain reaction (PCR) on faecal DNA from subjects belonging to different age groups. The analyses indicated that the number of different species (diversity) present in the human gut increased with age. The sequence information generated, provided the basis for design of 16S rRNA-directed oligonucleotide probes to specifically detect bacteria at various levels of phylogenetic hierarchy. The probes were tested for their specificity and used in whole-cell and dot-blot hybridisations. The applicability of the developed methods was demonstrated in several studies and the major outcomes are described.

    • Send article to Kindle

      To send this article to your Kindle, first ensure is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the or variations. ‘’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Molecular biological methods for studying the gut microbiota: the EU human gut flora project
      Available formats
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Molecular biological methods for studying the gut microbiota: the EU human gut flora project
      Available formats
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Molecular biological methods for studying the gut microbiota: the EU human gut flora project
      Available formats
Corresponding author
*Corresponding author: Professor M. Blaut, tel +49 33 20 08 84 70, fax +49 33 20 08 84 07, email
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

RD Berg (1996) The indigenous gastrointestinal microflora. Trends in Microbiology 4, 430435.

JH Cummings & GT Macfarlane (1997) Colonic microflora: nutrition and health. Nutrition 13, 476478.

J Doré , A Sghir , G Hannequart-Gramet , G Corthier & P Pochart (1998) Design and evaluation of a 16S rRNA-targeted oligonucleotide probe for specific detection and quantitation of human faecal Bacteroides populations. Systematics and Applied Microbiology 21, 6571.

SM Finegold , VL Sutter & GE Mathisen (1983) Normal indigenous intestinal flora. In Human intestinal microflora in health and disease, pp. 331 [DJ Hentges , editor]. New York: Academic Press.

HJ Harmsen , AC Wildeboer-Veloo , J Grijpstra , J Knol , JE Degener & GW Welling (2000) Development of 16S rRNA-based probes for the Coriobacterium group and the Atopobium cluster and their application for enumeration of Coriobacteriaceae in human feces from volunteers of different age groups. Applied and Environmental Microbiology 66, 45234527.

W Ludwig , O Strunk , S Klugbauer , N Klugbauer , M Weizenegger , J Neumaier , M Bachleitner & KH Schleifer (1998) Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19, 554568.

BL Maidak , GJ Olsen , N Larsen , R Overbeek , MJ McCaughey & CR Woese (1997) The RDP (Ribosomal Database Project). Nucleic Acids Research 25, 109110.

W Manz , R Amann , W Ludwig , M Vancanneyt & KH Schleifer (1996) Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum Cytophaga-Flavobacter-Bacteroides in the natural environment. Microbiology 142, 10971106.

GJ Olsen , DJ Lane , SJ Giovannoni , NR Pace & DA Stahl (1986) Microbial ecology and evolution: a ribosomal RNA approach. Annual Reviews of Microbiology 40, 337365.

IR Rowland (1988) Interactions of the gut microflora and the host in toxicology. Toxicology and Pathology 16, 147153.

H Schneider , A Schwiertz , MD Collins & M Blaut (1999) Anaerobic transformation of quercetin-3-glucoside by bacteria from the human intestinal tract. Archives of Microbiology 171, 8191.

H Schneider , R Simmering , L Hartmann , H Pforte & M Blaut (2000) Degradation of quercetin-3-glucoside in gnotobiotic rats associated with human intestinal bacteria. Journal of Applied Microbiology 89, 10271037.

A Schwiertz , G Le Blay & M Blaut (2000) Quantification of different Eubacterium spp in human fecal samples with species-specific 16S rRNA-targeted oligonucleotide probes. Applied and Environmental Microbiology 66, 375382.

A Sghir , G Gramet , A Suau , V Rochet , P Pochart & J Doré (2000) Quantification of bacterial groups within human fecal flora by oligonucleotide probe hybridization. Applied and Environmental Microbiology 66, 22632266.

CR Woese , O Kandler & ML Wheelis (1990) Towards a natural system of organisms: proposal for the domains Archaea, Bacteria and Eucarya. Proeedings of the National Academy of Sciences USA 87, 45764579.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *