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Production of tricarballylic acid by rumen microorganisms and its potential toxicity in ruminant tissue metabolism

  • James B. Russell (a1) and Neil Forsberg (a2)
Abstract

1. Rumen microorganisms convert trans-aconitate to tricarballylate. The following experiments describe factors affecting the yield of tricarballylate, its absorption from the rumen into blood and its effect on mammalian citric acid cycle activity in vitro.

2. When mixed rumen microorganisms were incubated in vitro with Timothy hay (Phleum praiense L.) and 6.7 mM-trans-aconitate, 64 % of the trans-aconitate was converted to tricarballylate. Chloroform and nirate treatments inhibited methane production and increased the yield of tricarballylate to 82 and 75% respectively.

3. Sheep given gelatin capsules filled with 20 g trans-aconitate absorbed tricarballylate and the plasma concentration ranged from 0.3 to 0.5 mM 9 h after administration. Feeding an additional 40 g potassium chloride had little effect on plasma tricarballylate concentrations. Between 9 and 36 h there was a nearly linear decline in plasma tricarballylate.

4. Tricarballylate was a competitive inhibitor of the enzyme, aconitate hydratase (aconitase; EC 4.2.1.3), and the inhibitor constant, KI, was 0.52 mM. This KI value was similar to the Michaelis-Menten constant (Km) of the enzyme for citrate.

5. When liver slices from sheep were incubated with increasing concentrations of tricarballylate, [I4C]acetate oxidation decreased. However, even at relatively high concentrations (8 mM), oxidation was still greater than 80% of the maximum. Oxidation of [I4C]acetate by isolated rat liver cells was inhibited to a greater extent by tricarballylate. Concentrations as low as 0.5 mM caused a 30% inhibition of citric acid cycle activity.

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References
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Allison M. J. & Reddy C. A. (1984). In Current perspectives in microbial ecologypp. 248256 [Klug M. J. and Reddy C. A., editors]. Washington, DC: American Society for Microbiology.
Baldwin R. L., Wood W. A. & Emery R. S. (1965). Biochimica et Biophysica Acta 97, 202213.
Barta A. L. (1973). Crop Science 13, 113114.
Bergmeyer H. U. & Klotsch H. (1965). In Methods of Enzymatic Analysis, pp. 99102 [Bergmeyer H. U., editor]. New York: Academic Press.
Bohman V. R., Horn F. P., Littledike E. T., Hurst J. G. & Griffen D. (1983 a). Journal of Animal Science 57, 13641373.
Bohman V. R., Horn F. P., Stewart B. A., Mathers A. C. & Grunes D. L. (1983 b). Journal of Animal Science 57, 13521363.
Bohman V. R., Lesperance A. L., Harding G. D. & Grunes D. L. (1969). Journal of Animal Science 29, 99102.
Bryant M. P. (1956). Journal of Bacteriology 72, 162167.
Burau R. G. & Stout P. R. (1965). Science 150, 766767.
Chen M. & Wolin M. J. (1977). Applied Environmental Microbiology 34, 756759.
Gill J. L. (1973). Journal of Dairy Science 56, 973977.
Goering H. K. & Van Soest P. J. (1970). Forage Fiber Analysis, US Department of Agriculture Handbook no. 379. Washington, DC: US Department of Agriculture.
Grunes D. L. (1967). Cornell Nutrition Conference for Feed Manufacturers. Buffalo N.Y. p. 105. Ithaca, NY: Cornell Agricultural Experiment Station.
Grunes D. L., Stout P. R. & Brownell J. R. (1970). Advances in Agronomy 22, 331374.
Hughes P. E. & Tove S. B. (1980). Journal of Biological Chemistry 255, 44474452.
Kennedy G. S. (1968). Australian Journal of Biological Sciences 21, 529538.
Kirkby E. A. (1969). In Ecological Aspects of the Mineral Nutrition of Plants, pp. 215235 [Rorison I. H., editor]. Oxford:Blackwell Scientific Publishers.
Linehan B., Scheifinger C. C. & Wolin M. J. (1978). Applied Environmental Microbiology 35, 317322.
Lomba F., Fumiere I., Chauvaux G., Binot H. & Bienfet V. (1969 a). Annales de Medicine et Veterinare 113, 2223.
Lomba F., Fumiere I., Chauvaux G., Binot H. & Bienfet V. (1969 b). Annates de Medicine et Veterinare 113, 90100.
Mayland H. F. & Grunes D. L. (1979). In Grass Tetany, pp. 123175 [Rendig V. V. and Grunes D. L., editors]. Madison, Wisconsin: American Society of Agronomy.
Morrison J. F. (1954). Australian Journal of Experimental Biology 32, 867876.
Paynter M. J. B. & Elsden S. R. (1970). Journal of General Microbiology 61, 17.
Perkin-Elmer (1982). Analytical Methods for Atomic Absorption Spectrophotometry. Norwalk, Conn.: Perkin-Elmer.
Peters R. A. (1957). Advances in Enzymology 18, 113119.
Peters R. A. & Wilson T. H. (1952). Biochimica et Biophysica Acta 9, 310315.
Prior R. L., Grunes D. L., Patterson R. P., Smith F. W., Mayland H. F. & Visek W. J. (1973). Journal of Agricultural and Food Chemistry 21, 1377.
Russell J. B. (1985). Applied Environmental Microbiology 49, 120126.
Russell J. B. & Van Soest P. J. (1984). Applied Environmental Microbiology 41, 155159.
Scheifinger C. C. & Wolin M. J. (1973). Applied Microbiology 26, 789795.
Scotto K. C., Bohman V. R. & Lesperance A. L. (1971). Journal of Animal Science 32, 354358.
Seglen P. O. (1976). In Methods in CellBiology, vol. 13, pp. 2983 [Prescott D. M., editor]. New York: Academic Press.
Sigma Chemical Co. (1977). Aconitase Methodology Sheet A-5384. St Louis, MO: Sigma Chemical Co.
Stout P. R., Brownell J. R. & Bureau R. G. (1967). Agronomy Journal 59, 2124.
Wolin M. J. (1975). In Digestion and Metabolism in the Ruminant, pp. 134148 [McDonald I. W.&Warner A. C. L., editors]. Armidale, NSW, Australia: University of New England Publishing Unit.
Wolin M. J., Wolin E. A. & Jacobs N. J. (1961). Journal of Bacteriology 8, 911917.
Wright D. E. (1971). Applied Microbiology 21, 165168.
Wright D. E. & Wolff J. E. (1969). New Zealand Journal of Agricultural Research 12, 287292.
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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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