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Gene-flow between populations of cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) is highly variable between years

  • K.D. Scott (a1), K.S. Wilkinson (a1), N. Lawrence (a1), C.L. Lange (a1), L.J. Scott (a1), M.A. Merritt (a1), A.J. Lowe (a1) and G.C Graham (a1)...
Abstract
Abstract

Both large and small scale migrations of Helicoverpa armigera Hübner in Australia were investigated using AMOVA analysis and genetic assignment tests. Five microsatellite loci were screened across 3142 individuals from 16 localities in eight major cotton and grain growing regions within Australia, over a 38-month period (November 1999 to January 2003). From November 1999 to March 2001 relatively low levels of migration were characterized between growing regions. Substantially higher than average gene-flow rates and limited differentiation between cropping regions characterized the period from April 2001 to March 2002. A reduced migration rate in the year from April 2002 to March 2003 resulted in significant genetic structuring between cropping regions. This differentiation was established within two or three generations. Genetic drift alone is unlikely to drive genetic differentiation over such a small number of generations, unless it is accompanied by extreme bottlenecks and/or selection. Helicoverpa armigera in Australia demonstrated isolation by distance, so immigration into cropping regions is more likely to come from nearby regions than from afar. This effect was most pronounced in years with limited migration. However, there is evidence of long distance dispersal events in periods of high migration (April 2001–March 2002). The implications of highly variable migration patterns for resistance management are considered.

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*Fax: +61 7 3365 1861 E-mail: k.scott@uq.edu.au
References
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Arguedas N., Parker P.G. (2000) Seasonal migration and genetic population structure in house wrens. Condor 102, 517528.
Britten H.B., Glasford J.W. (2002) Genetic population structure of the Dakota skipper (Lepidoptera: Hesperia dacotae): a North American native prairie obligate. Conservation Genetics 3, 363374.
Coombs M. (1997) Tethered-flight and age-related reproductive performance of Helicoverpa punctigera (Wallengren) and H. armigera (Hübner) (Lepidoptera: Noctuidae). Australian Journal of Zoology 45, 409422.
Cornuet J.M., Piry S., Luikart G., Estoup A. & Solignac M. (1999) New methods employing multilocus genotypes to select or exclude populations as origins of individuals. Genetics 153, 19892000.
Daly J.C. & Gregg P. (1985) Genetic variation in Heliothis in Australia: species identification and gene-flow in two pest species H. armigera (Hübner) and H. punctigera Wallengren (Lepidoptera: Noctuidae). Bulletin of Entomological Research 75, 169184.
Excoffier L., Smouse P.R., Quattro J.M. (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction sites. Genetics 131, 479491.
Fitt G.P. (1989) The ecology of Heliothis species in relation to agroecosystems. Annual Review of Entomology 34, 1752.
Fitt G.P., Daly J.C. (1990) Abundance of overwintering pupae and the spring generation of Helicoverpa spp. (Lepidoptera; Noctuidae) in northern New South Wales, Australia: implications for pest management. Journal of Economic Entomology 83, 18271836.
Fitt G.P., Dillon M.L. (1993) Spatial population modelling of Helicoverpa spp.: studies of adult behaviour and movement. pp. 449451in Corey S.A., Dall D.J. & Milne W.M., (Eds) Pest control and sustainable agriculture. Melbourne, CSIRO Publications.
Fitt G.P., Dillon M.L., Hamilton J.G. (1995) Spatial dynamics of Helicoverpa populations in Australia: simulation modelling and empirical studies of adult movement. Computers and Electronics in Agriculture 13, 177192.
Forrester N.W., Cahill M., Bird L.J., Layland J.K. (1993) Management of pyrethroid and endosulfan resistance in Helicoverpa armigera (Lepidoptera: Noctuidae). Bulletin of Entomological Research Supp. series1, 132.
Gregg P.C. (1993) Pollen as a marker for migration of Helicoverpa armigera and H. punctigera (Lepidoptera: Noctuidae) from western Queensland. Australian Journal of Ecology 18, 209219.
Gunning R.V., Easton C.S. (1994) Endosulphan resistance in Helicoverpa armigera (Lepidoptera: Noctuidae) in Australia. Journal of the Australian Entomological Society 33, 381383.
Hartl D.L., Clark A.G. (1997) Principles of population genetics 3rd edn Sunderland, Massachusetts Sinauer Associates Inc
Hou M. & Sheng C. (1999) Fecundity and longevity of Helicoverpa armigera (Lepidoptera: Noctuidae): effects of multiple mating. Journal of Economic Entomology 92, 569573.
Ji Y.-J., Zhang D.-X., Hewitt G.M., Kang L., Li D.M. (2003) Polymorphic microsatellite loci for the cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) and some remarks on their isolation. Molecular Ecology Notes 3, 102104.
Johannesen J., Schwing U., Seufert W., Seitz A. & Veith M. (1997) Analysis of gene-flow and habitat patch network for Chazara briseis (Lepidoptera: Satyridae) in an agricultural landscape. Biochemical Systematics and Ecology 25, 419427.
Meglecz E., Pecsenye K., Varga Z. & Solignac M. (1998) Comparison of differentiation pattern at allozyme and microsatellite loci in Parnassius mnemosyne (Lepidoptera) populations. Hereditas 128, 95103.
Michalakis Y. & Excoffier L. (1996) A generic estimation of population subdivision using distances between alleles with special reference for microsatellite loci. Genetics 142, 10611064.
Miller S.A., Dykes D.D., Polesky H.F. (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research 16, 1215
Nibouche S., Buès R., Toubon J.-F. & Poitout S. (1998) Allozyme polymorphism in the cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae): comparison of African and European populations. Heredity 80, 438445.
Paetkau D., Slade R., Burden M. & Estoup A. (2004) Genetic assignment methods for the direct, real-time estimation of migration rate: a simulation-based exploration of accuracy and power. Molecular Ecology 13, 5565.
Peakall R. & Smouse P.E. (2001) GenA1Ex Ver.5: Genetic analysis in Excel. Population genetic software for teaching and research. Australian National University, Canberra, Australia. http://www.anu.edu.au/BoZo/GenA1Ex/
Peakall R., Smouse P.E., Huff D.R. (1995) Evolutionary implications of allozyme and RAPD variation in diploid populations of buffalograss (Buchloë dactyloides (Nutt. Engelm.). Molecular Ecology 4, 135147.
Piry S., Alapetite A., Cornuet J.-M., Paetkau D., Baudouin L. & Estoup A. (2004) GeneClass2: a software for genetic assignment and first generation migrants detection. Journal of Heredity 95, 536539.
Rannala B., Mountain J.L. (1997) Detecting immigration by using multilocus genotypes. Proceedings of the National Academy of Sciences of the USA 94, 91979201.
Rochester W.A. (1999) The migration systems of Helicoverpa punctigera (Wallengren) and Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) in Australia. PhD thesis, The University of Queensland, Brisbane, Australia.
Rochester W.A., Dillon M.L., Fitt G.P., Zalucki M.P. (1996) A simulation model of the long-distance migration of Helicoverpa spp. moths. Ecological Modelling 86, 151156.
Saito O. (2000) Flight activity of the cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), by aging and copulation as measured by flight actograph. Applied Entomology and Zoology 35, 5361.
Scott K.D., Wilkinson K.S., Merritt M.A., Scott L.J., Lange C.L., Schutze M.K., Kent J.K., Merritt D.J., Grundy P.R., Graham G.C. (2003) Genetic shifts in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) over a year in the Dawson/Callide Valleys. Australian Journal of Agricultural Research 54, 739744.
Scott K.D., Lange C.L., Scott L.J., Graham G.C. (2004) Isolation and characterisation of microsatellite loci from Helicoverpa armigera Hübner (Lepidoptera: Noctuidae). Molecular Ecology Notes 4, 204205.
Singh A.K. & Rembold H. (1989) Oviposition behaviour of Heliothis armigera (Lepidoptera: Noctuidae) in relation to the day–night cycle. Insect Science and its Applications 10, 393400.
Smouse P.E., Long J.C. (1992) Matrix correlation analysis in anthropology and genetics. Yearbook of Physical Anthropology 35, 187213.
Smouse P.E., Long J.C., Sokal R.R. (1986) Multiple regression and correlation extensions of the Mantel test of matrix correspondence. Systematic Zoology 35, 627632.
Stokes N.H., McKechnie S.W., Forrester N.W. (1997) Multiple allelic variation in a sodium channel gene from populations of Australian Helicoverpa armigera (Hübner) (Lepidoptera; Noctuidae) detected via temperature gradient gel electrophoresis. Australian Journal of Entomology 36, 191196.
Tan S., Chen X., Zhang A. & Li D. (2001) Isolation and characterization of DNA microsatellites from cotton bollworm (Helicoverpa armigera, Hübner). Molecular Ecology Notes 1, 243244.
Wardill T.J., Scott K.D., Graham G.C., Zalucki M.P. (2004) Isolation and characterisation of microsatellite loci from Chiasmia assimilis Warren (Lepidoptera: Geometridae). Molecular Ecology Notes 4, 361363.
Wilson G.A. & Rannala B. (2003) Bayesian inference of recent migration using multilocus genotypes. Genetics 163, 11771191.
Wu K. & Guo Y. (1996) Flight activity in Helicoverpa armigera. Acta Entomologica Sinica 16, 612617.
Zhou X., Faktor O., Applebaum S.W. & Coll M. (2000) Population structure of the pestiferous moth Helicoverpa armigera in the eastern Mediterranean using RAPD analysis. Heredity 85, 251256.
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Bulletin of Entomological Research
  • ISSN: 0007-4853
  • EISSN: 1475-2670
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