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The Effects of Maternal Deprivation on the Hippocampal Structure in Adult Rats

Published online by Cambridge University Press:  02 December 2014

Pınar Karakaş ,
Memduha Gülhal Bozkır ,
Fahri Dere ,
Enver Melik ,
Emine Babar Melik ,
Mehmet Kaya  and
Sait Polat
Pınar Karakaş*
Affiliation:
Çukurova University, Faculty of Medicine, Department of Anatomy, Adana, Turkey
Memduha Gülhal Bozkır
Affiliation:
Çukurova University, Faculty of Medicine, Department of Anatomy, Adana, Turkey
Fahri Dere
Affiliation:
Çukurova University, Faculty of Medicine, Department of Anatomy, Adana, Turkey
Enver Melik
Affiliation:
Department of Physiology, Adana, Turkey
Emine Babar Melik
Affiliation:
Department of Physiology, Adana, Turkey
Mehmet Kaya
Affiliation:
Department of Histology-Embryology, Adana, Turkey
Sait Polat
Affiliation:
Department of Histology-Embryology, Adana, Turkey
*
Çukurova University, Faculty of Medicine, Department of Anatomy, 01330, Adana, Turkey
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Abstract

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Objectives:

To examine the ultrastructural effects of maternal deprivation during developmental periods of limbi-chypothalamo-pituitary-adrenal system on hippocampal dendritic structures in adult rats.

Methods:

The experiments were carried out with male and female wistar rats in our department. The rats were mated and, after birth, the pups were divided into four groups. The first group (control group) pups remained undisturbed with their dam until postweaning day 22. Maternal deprived groups were separated from their dams for 24 hours at postnatal day 4, 9 and 18. The subjects were provided with food and water ad libitum until 3-months-of-age. At the third month, the rats were transcardially perfused, samples were taken from CA1 and CA3 regions of the hippocampus. Tissues were prepared for electron microscopy.

Results:

When the data were analyzed, there were no differences between male and female rats in both ultrastructure and semiquantitative analysis of axodendritic synapses. The ultrastructure of Group 1 was seen as normal while in the second Group some neurons nuclear envelope made deep invagination into the nucleus. Additionally, axodendritic synapses were found normal. In Group 3, micrographs and axodendritic synapses were showed normal structure. However, in Group 4 in some neurons invaginations were seen similar to Group 2. Axodendritic synapses were found to be normal.

Conclusion:

These experiments establish that MD in rats produces slight ultrastructural changes and decreases the number of synapses in CA1 and CA3 subregions of the hippocampus.

Type
Original Article
Information
Canadian Journal of Neurological Sciences , Volume 36 , Issue 3 , May 2009 , pp. 356 - 362
Copyright
Copyright © The Canadian Journal of Neurological 2009

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