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Embryonic ventricular diastolic and systolic pressure-volume relations

  • Bradley B. Keller (a1), Joseph P. Tinney (a1) and Norman Hu (a1)

The embryonic heart and vascular bed interact dynamically to support rapid growth of the embryo during cardiovascular development. Pressure-volume relations define ventricular function during alterations in loading conditions. We analyzed these relationships in the embryonic heart in order to define ventricular function and the response of the ventricle and vascular bed to acute changes in preload. We simultaneously measured ventricular pressure and recorded 60 video images per second in n≥6 stage 16, 18 and 21 white Leghorn chick embryos at baseline and during the infusion of 1–2 microliters of physiologic buffer into the venous sinus (sinus venosus). Ventricular tetany was then induced with the topical application of 2 Molar sodium chloride. Video fields were traced for ventricular pressure and epicardial cross-sectional area. Cross-sectional area was converted to volume assuming ellipsoid geometry, and cavity volume was calculated as total volume minus wall volume derived from the tetanized heart. We defined end-diastole at the onset of ventricular contraction and end-systole at maximum pressure/volume ratio. Stroke volume increased linearly with end-diastolic volume. End-diastolic pressure-volume relations were positive and linear, and end-systolic pressure-volume relations were curvilinear downward. Arterial elastance decreased with growth of the embryo and with volume infusion. Pressure-volume loop area, an index of consumption of energy, doubled between the embryonic stages. Thus, embryonic ventricular pressure-volume relations define diastolic and systolic function at rest and in response to altered preload.

Corresponding author
Division of Pediatric Cardiology,University of Rochester,601 Elmwood Avenue,Box 631,Rochester,NY 14642,USA. Tel. (716) 275-6096; Fax. (716) 275-7436.
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N Hu , EB. Clark Hemodynamics of the stage 12 to stage 29 chick embryo. Circ Res 1989; 65: 16651670.

DF Teitel , P Klautz , P Steednijk , ET van der Velde , F van Bel , J. Baan The end-systolic pressure-volume relationship in the newborn lamb: Effects of loading and inotropic interventions. Pediatr Res 1991; 29: 473482.

K Sunagawa , K Sagawa , WL. Maughan Ventricular interaction with the vascular system in terms of pressure-volume relationships. In: FCP Yin (ed). Ventricular Vascular Coupling: Clinical, Physiological, and Engineering Aspects. Springer-Verlag, New York, 1987, pp 210239.

BB Keller , N Hu , EB. Clark Correlation of ventricular area, perimeter, and conotruncal diameter with ventricular mass and function in the stage 12 to 24 chick embryo. Circ Res 1990; 66: 109114.

BB Keller , N Hu , PJ Serrino , EB. Clark Ventricular pressure-area loop characteristics in the stage 16 to 24 chick embryo. Circ Res 1991; 68: 226231.

V Hamburger , HL. Hamilton A series of normal stages in the development of the chick embryo. J Morphol 1951; 88: 4992.

N Hu , DM Connuck , BB Keller , EB. Clark Diastolic filling characteristics in the stage 12 to 27 chick embryo ventricle. Pediatr Res 1991; 29: 334337.

M Nakazawa , S Miyagawa , A Takao , N Hu , EB. Clark Hemodynamic effects of environmental hyperthermia in the stage 18, 21, and 24 chick embryo. Pediatr Res 1986; 20: 12131215.

BM Patten , TC Kramer , A. Barry Valvular action in the embryonic chick heart by localized apposition of endocardial masses. Anat Rec 1948; 102: 299311.

LK Waldman , D Nosan , F Villarreal , JW. Covell Relation between transmural deformation and local myofiber direction in canine left ventricle. Circ Res 1988; 63: 550562.

CAF Rasmussen Jr, JL Sutko , WH. Barry Effects of ryanodine and caffeine on contractility, membrane voltage, and calcium exchange in cultured heart cells. Circ Res 1987; 60: 495504.

WL Maughan , K Sunagawa , D Burkhoff , K. Sagawa Effect of arterial impedance changes on the end-systolic pressure-volume relation. Circ Res 1985; 54: 595602.

KG Zahka , N Hu , KP Brin , FCP Yin , EB. Clark Aortic impedance and hydraulic power in the chick embryo from stages 18 to 29. Circ Res 1989; 64: 10911095.

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Cardiology in the Young
  • ISSN: 1047-9511
  • EISSN: 1467-1107
  • URL: /core/journals/cardiology-in-the-young
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