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Colonization resistance of the digestive tract in conventional and antibiotic-treated mice

  • D. Van der Waaij (a1), J. M. Berghuis-de Vries (a1) and J. E. C. Lekkerkerk-van der Wees (a1)
  • DOI:
  • Published online: 01 May 2009

The effect of oral administration of antibiotics on the intestinal flora of conventional mice and their resistance to colonization by orally introduced Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa was studied. Colonization resistance (CR) was expressed as the log of the oral bacterial dose followed by a persistent take in 50 % of the contaminated animals. The intestinal flora was virtually eliminated by the antibiotics and this elimination was accompanied by a precipitous fall of CR. CR gradually returned to normal values during the period of repopulation of the intestinal tract by the organisms surviving the treatment. Antibiotic treatment resulted in the disappearance of Enterobacteriaceae, enterococci, staphylococci and yeasts and, under appropriate housing conditions, the animals remained free of these organisms indefinitely. Germ-free mice contaminated with the intestinal flora of an antibiotic-treated animal and their offspring housed in a germ-free isolator showed high values of CR. Their intestinal flora consisted of anaerobic bacteria only. Apparently, these anaerobes are responsible for CR in these and in conventional mice.

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G. D. Abrams & J. E. Bishop (1967). Effect of the normal microbial flora on gastro-intestinal motility. Proceedings of the Society for Experimental Biology and Medicine 126, 301.

M. Bohnhoff & C. P. Miller (1962). Enhanced susceptibility to Salmonella infection in streptomycin-treated mice. Journal of Infectious Diseases 111, 117.

M. Bohnhoff , C. P. Miller & W. R. Martin (1964 b). Resistance of the mouse's intestinal tract to experimental Salmonella infection. II. Factors responsible for its loss following streptomycin treatment. Journal of Experimental Medicine 120, 817.

D. C. Savage & R. Dubos (1968). Alterations in the mouse cecum and its flora produced by antibacterial drugs. Journal of Experimental Medicine 128, 97.

R. W. Schaedler , R. Dubos & R. Costello (1965). Association of germfree mice with bacteria isolated from normal mice. Journal of Experimental Medicine 122, 77.

P. H. A. Sneath (1956). Cultural and biochemical characteristics of the genus Chromobacterium. Journal of General Microbiology 15, 70.

D. van der Waaij (1968). The persistent absence of Enterobacteriaceae from the intestinal flora of mice following antibiotic treatment. Journal of Infectious Diseases 118, 32.

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Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
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