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A randomized controlled trial of vitamin D3 supplementation for the prevention of symptomatic upper respiratory tract infections

  • M. LI-NG (a1), J. F. ALOIA (a1), S. POLLACK (a1), B. A. CUNHA (a2), M. MIKHAIL (a1), J. YEH (a1) and N. BERBARI (a3)...

Vitamin D has been shown to be an important immune system regulator. Vitamin D insufficiency during winter may cause increased susceptibility to upper respiratory tract infections (URIs). To determine whether vitamin D supplementation during the winter season prevents or decreases URI symptoms, 162 adults were randomized to receive 50 μg vitamin D3 (2000 IU) daily or matching placebo for 12 weeks. A bi-weekly questionnaire was used to record the incidence and severity of URI symptoms. There was no difference in the incidence of URIs between the vitamin D and placebo groups (48 URIs vs. 50 URIs, respectively, P=0·57). There was no difference in the duration or severity of URI symptoms between the vitamin D and placebo groups [5·4±4·8 days vs. 5·3±3·1 days, respectively, P=0·86 (95% CI for the difference in duration −1·8 to 2·1)]. The mean 25-hydroxyvitamin D level at baseline was similar in both groups (64·3±25·4 nmol/l in the vitamin D group; 63·0±25·8 nmol/l in the placebo group; n.s.). After 12 weeks, 25-hydroxyvitamin D levels increased significantly to 88·5±23·2 nmol/l in the vitamin D group, whereas there was no change in vitamin D levels in the placebo group. There was no benefit of vitamin D3 supplementation in decreasing the incidence or severity of symptomatic URIs during winter. Further studies are needed to determine the role of vitamin D in infection.

Corresponding author
*Author for correspondence: J. F. Aloia, M.D., Winthrop University Hospital, 222 Station Plaza North, Suite 510, Mineola, NY 11501, USA. (Email:
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2.M Visser , DJ Deeg , P Lips . Low vitamin D and high parathyroid hormone levels as determinants of loss of muscle strength and muscle mass (sarcopenia): the Longitudinal Aging Study Amsterdam. Journal of Clinical Endocrinology and Metabolism 2003; 88: 57665772.

3.ER Bertone-Johnson , Plasma 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D and risk of breast cancer. Cancer Epidemiology Biomarkers & Prevention 2005; 14: 19911997.

5.DM Freedman , M Dosemeci , K McGlynn . Sunlight and mortality from breast, ovarian, colon, prostate, and non-melanoma skin cancer: a composite death certificate based case-control study. Occupational & Environmental Medicine 2002; 59: 257262.

6. H. Schmidt-Gayk , Serum 25-hydroxycalciferol in myocardial infarction. Atherosclerosis 1977; 26: 5558.

7. M Cutolo , Vitamin D in rheumatoid arthritis. Autoimmunity Reviews 2007; 7: 5964.

8.M Cutolo , K Otsa . Vitamin D, immunity and lupus [Review]. Lupus 2008; 17: 610.

9. TT Wang , Cutting edge: 1,25-dihydroxyvitamin D3 is a direct inducer of antimicrobial peptide gene expression. Journal of Immunology 2004; 173: 29092912.

10.ME Klotman , TL Chang . Defensins in innate antiviral immunity. Nature Reviews Immunology 2006; 6: 447456.

11. E Leikina , Carbohydrate-binding molecules inhibit viral fusion and entry by crosslinking membrane glycoproteins. Nature Immunology 2005; 6: 9951001.

12.PT Liu , Toll-like receptor triggering of a vitamin D-mediated human antimicrobial response. Science 2006; 311: 17701773.

13.SS Harris , B Dawson-Hughes . Seasonal changes in plasma 25-hydroxyvitamin D concentrations of young American black and white women. American Journal of Clinical Nutrition 1998; 67: 12321236.

14.RE Hope-Simpson , DB Golubev . A new concept of the epidemic process of influenza A virus. Epidemiology and Infection 1987; 99: 554.

15. RE Hope-Simpson . The Transmission of Epidemic Influenza. New York: Plenum Press, 1992.

16.AM Fendrick , The economic burden of non-influenza-related viral respiratory tract infection in the United States. Archives of Internal Medicine 2003; 163: 487494.

18.JM Lemire . Immunomodulatory role of 1,25-dihydroxyvitamin D3. Journal of Cell Biochemistry 1992; 49: 2631.

20.JF Aloia , A randomized controlled trial of vitamin D3 supplementation in African American women. Archives of Internal Medicine 2005; 165: 16181623.

23.R Vieth , The urgent need to recommend an intake of vitamin D that is effective. American Journal of Clinical Nutrition 2007; 85: 649650.

24.J Chubak , Moderate-intensity exercise reduces the incidence of colds among postmenopausal women. American Journal of Medicine 2006; 119: 937942.

25.DC Nieman , Physical activity and immune function in elderly women. Medicine & Science in Sports & Exercise 1993; 25: 823831.

28. A Avenell , Vitamin D supplementation to prevent infections: a sub-study of a randomised placebo-controlled trial in older people [RECORD trial, ISRCTN 51647438]. Age and Ageing 2007; 36: 574577.

31.MJ Barger-Lux , Vitamin D and its major metabolites: serum levels after graded oral dosing in healthy men. Osteoporosis International 1998; 8: 222230.

32.AC Looker , Serum 25-hydroxyvitamin D status of adolescents and adults in two seasonal subpopulations from NHANES III. Bone 2002; 30: 771777.

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Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
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