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Genetic diversity and antibiotic resistance in Shigella dysenteriae and Shigella boydii strains isolated from children aged

  • A. M. EL-GENDY (a1), A. MANSOUR (a1), M. A. WEINER (a1), G. PIMENTEL (a2), A. W. ARMSTRONG (a1), S. Y. N. YOUNG (a1), N. ELSAYED (a3) and J. D. KLENA (a1)...
Summary
SUMMARY

Diversity within Shigella dysenteriae (n=40) and Shigella boydii (n=30) isolates from children living in Egypt aged <5 years was investigated. Shigella-associated diarrhoea occurred mainly in summer months and in children aged <3 years, it commonly presented with vomiting and fever. Serotypes 7 (30%), 2 (28%), and 3 (23%) accounted for most of S. dysenteriae isolates; 50% of S. boydii isolates were serotype 2. S. dysenteriae and S. boydii isolates were often resistant to ampicillin, chloramphenicol and tetracycline (42%, 17%, respectively), although resistance varied among serotypes. Pulsed-field gel electrophoresis separated the isolates into distinct clusters correlating with species and serotype. Genetic differences in trimethoprim/sulfamethoxazole and β-lactam-encoding resistance genes were also evident. S. dysenteriae and S. boydii are genetically diverse pathogens in Egypt; the high level of multidrug resistance associated with both pathogens and resistance to the most available inexpensive antibiotics underlines the importance of continuing surveillance.

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Corresponding author
*Author for correspondence: Professor J. D. Klena, Unit 7300, Box 060 DPO AP 96521-0050 USA. (Email: irc4@cn.cdc.gov)
References
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1.Ram PK, et al. Part II. Analysis of data gaps pertaining to Shigella infections in low and medium human development index countries, 1984–2005. Epidemiology and Infection 2008; 136: 577603.
2.Kotloff KL, et al. Global burden of Shigella infections: implications for vaccine development and implementation of control strategies. Bulletin of the World Health Organization 1999; 77: 651666.
3.Niyogi SK. Shigellosis. Journal of Microbiology 2005; 43: 133143.
4.Acheson DWK, Donohue-Rolfe A, Keusch GT. The family of shiga and shiga-like toxins. In: Alouf JE, Freer AH, eds. Sourcebook of Bacterial Protein Toxins. New York: Academic Press, 1991, pp. 415433.
5.Kernéis S, et al. A look back at an ongoing problem: Shigella dysenteriae type 1 epidemics in refugee settings in Central Africa (1993–1995). PLoS One 2009; 4: e4494.
6.Putnam SD, et al. Antimicrobial susceptibility trends among Escherichia coli and Shigella spp. isolated from rural Egyptian paediatric populations with diarrhoea between 1995 and 2000. Clinical Microbiology and Infection 2004; 10: 804810.
7.Abu-Elyazeed R, et al. Epidemiology of Shigella-associated diarrhea in rural Egyptian children. American Journal of Tropical Medicine and Hygiene 2004; 71: 367372.
8.Wasfy MO, et al. Isolation and antibiotic susceptibility of Salmonella. Shigella, and Campylobacter from acute enteric infections in Egypt. Journal of Health, Population and Nutrition 2000; 18: 3338.
9.Zaki AM, et al. The detection of enteropathogens in acute diarrhea in a family cohort population in rural Egypt. American Journal of Tropical Medicine and Hygiene 1986; 35: 10131022.
10.Wierzba TF, et al. Clinic-based surveillance for bacterial- and rotavirus-associated diarrhea in Egyptian children. American Journal of Tropical Medicine and Hygiene 2006; 74: 148153.
11.Ahmed SF, et al. Epidemiology and genetic characterization of Shigella flexneri strains from three paediatric populations in Egypt (2000–2004). Epidemiology and Infection 2006; 134: 12371248.
12.El-Gendy A, et al. Identification of Shigella flexneri subserotype 1c in rural Egypt. Journal of Clinical Microbiology 1999; 37: 873874.
13.Sack DA, Lyke C, McLaughlin C. Antimicrobial Resistance in Shigellosis, Cholera and Campylobacteriosis. In: Sack DA, Lyke C, McLaughlin C, eds. World Health Organization, CDS/CSR/DRS/2001, pp. 151.
14.Bopp CA, et al. Escherichia, Shigella, and Salmonella. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, eds. Manual of Clinical Microbiology, 7th edn. Washington, DC: American Society for Microbiology, 1999, pp. 459474.
15.Rao MR, et al. High disease burden of diarrhea due to enterotoxigenic Escherichia coli among rural Egyptian infants and young children. Journal Clinical Microbiology 2003; 41: 48624864.
16.CLSI. Performance standards for antimicrobial susceptibility testing; fifteenth informational supplement 100-S15. Clinical and Laboratory Standards Institute, 2005.
17.Houng H-SH, Sethabutr O, Echeverria PA. simple polymerase chain reaction technique to detect and differentiate Shigella and enteroinvasive Escherichia coli in human feces. Diagnostic Microbiology and Infectious Diseases 1997; 28: 1925.
18.Goldstein C, et al. Incidence of class 1 and class 2 integrases in clinical and commensal bacteria from livestock, companion animals and exotics. Antimicrobial Agents Chemotherapy 2001; 45: 723726.
19.Chen S, et al. Characterization of multiple-antimicrobial-resistant Salmonella serovars isolated from retail meats. Applied Environmental Microbiology 2004; 70: 17.
20.Kerrn MB, et al. Susceptibility of Danish Escherichia coli strains isolated from urinary tract infections and bacteraemia, and distribution of sul genes conferring sulphonamide resistance. Journal of Antimicrobial Chemotherapy 2002; 50: 513516.
21.Ribot EM, et al. Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet. Foodborne Pathogens and Disease 2006; 3: 5967.
22.Vauterin P. Dendrograms and PFGE Analysis. In: 10th PulseNet Annual Meeting. Miami, FL, 6 April 2006 (http://www.aphl.org/profdev/conferences/proceedings/Pages/10thAnnualPulseNetUpdateMeeting.aspx). Accessed 5 December 2010.
23.Kernéis S, et al. A look back at an ongoing problem: Shigella dysenteriae Type 1 epidemics in refugee settings in Central Africa (1993–1995). PLoS One 2009; 4: e4494.
24.Löfdahl M, et al. An outbreak of Shigella dysenteriae in Sweden, May–June 2009, with sugar snap peas as the suspected source. Eurosurveillance 2009; 14: 13.
25.Smith AM, et al. Analysis of a temporal cluster of Shigella boydii isolates in Mpumalanga, South Africa, November to December 2007. Journal of Infection in Developing Countries 2009; 3: 6570.
26.Khan AI, et al. Shigella serotypes among hosptialized patients in urban Bangladesh and their antimicrobial resistance. Epidemiology and Infection 2004; 132: 773777.
27.Zafar A, et al. Frequency of isolation of various subtypes and antimicrobial resistance of Shigella from urban slums of Karachi, Pakistan. International Journal of Infectious Diseases 2009; 13: 668672.
28.Von Seidlin L, et al. A multicentre study of Shigella diarrhoea in six Asian countries: disease burden, clinical manifestations and microbiology. PLoS Medicine 2006; 9: e353.
29.Al-Moyed KA, et al. Increasing single and multi-antibiotic resistance in Shigella species isolated from shigellosis patients in Sana'a, Yemen. Saudi Medical Journal 2006; 27: 11571160.
30.Karacan C, et al. Evaluation of shigellosis in a Turkish children's hospital. Pediatrics International 2007; 49: 589592.
31.Farshad S, et al. Characterization of Shigella strains in Iran by plasmid profile analysis and PCR amplification of ipa genes. Journal of Clinical Microbiology 2006; 44: 28792883.
32.Taneja N. Changing epidemiology of shigellosis and emergence of ciprofloxacin-resistant Shigellae in India. Journal of Clinical Microbiology 2007; 45: 678679.
33.Khatun F, et al. Changing species distribution and antimicrobial susceptibility pattern of Shigella over a 29-year period (1980–2008). Epidemiology and Infection 2010; 18: 17.
34.Dubois V, et al. High genetic stability of integrons in clinical isolates of Shigella spp. of worldwide origin. Antimicrobial Agents Chemotherapy 2007; 51: 13331340.
35.Gerner-Smidt P, et al. Molecular surveillance of shiga toxigenic Escherichia coli O157 by Pulsenet USA. Journal of Food Protection 2005; 68: 1926–1231.
36.Sánchez S, et al. Longitudinal study of Shiga toxin-producing Escherichia coli shedding in sheep feces: persistence of specific clones in sheep flocks. Applied Environmental Microbiology 2009; 75: 17691773.
37.Talukder KA, et al. Genetic relatedness of ciprofloxacin-resistant Shigella dysenteriae type 1 strains isolated in south Asia. Journal of Antimicrobial Chemotherapy 2004; 54: 730734.
38.Jeong HJ, et al. Acanthamoeba: could it be an environmental host of Shigella? Experimental Parasitiology 2007; 115: 181186.
39.Lorenzo-Morales J, et al. Acanthamoeba isolates belonging to T1, T2, T3, T4 and T7 genotypes from environmental freshwater samples in the Nile Delta region, Egypt. Acta Tropica 2006; 100: 6369.
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Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
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