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The growth in vitro of vaccinia virus in chick embryo chorio-allantoic memberane, minced embryo and cell suspensions

  • H. B. Maitland (a1) and D. I. Magrath (a1)

The growth curve of rabbit skin-adapted vaccinia virus in the chick chorioallantoic membrane incubated in Hanks' solution showed a drop in titre of virus for about 10 hr. followed by growth. At least 25% of virus, sometimes more, remained infective. A similar fall in titre was observed in heated membranes in which the virus did not grow and this occurred also when membranes, either normal or heated, were infected and disintegrated before incubation.

The growth curve of virus in minced chick-embryo was similar to that in chorioallantoic membrane.

Virus in cell suspensions prepared from chick embryo and incubated in a nutrient medium showed only a small loss of infectivity before growth in some experiments and rarely dropped below 65–70 % of the original titre in others.

These results throw considerable doubt on the view that loss of infectivity preceding growth of vaccinia virus should be interpreted as an essential part of a growth cycle.

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S. G. Anderson (1954). The growth curve of vaccinia virus on the chorioallantois. Aust. J. exp. Biol. med. Sci. 32, 633.

J. O. W. Bland & C. F. Robinow (1939). The inclusion bodies of vaccinia and their relationship to the elementary bodies studied in cultures of the rabbit's cornea. J. Path. Bact. 48, 381.

R. Dulbecco & M. Vogt (1954). Plaque formation and isolation of pure lines with poliomyelitis viruses. J. exp. med. 99, 167.

W. H. Gaylord & J. L. Melnick (1953). Intracellular forms of pox viruses as shown by the electron microscope (vaccinia, ectromelia, molluscum contagiosum). J. exp. Med. 98, 157.

C. L. Hoagland , J. E. Smadel & T. M. Rivers (1940). Constituents of elementary bodies of vaccinia. I. Certain basic analyses and observations on lipid components of the virus. J. exp. Med. 71, 737.

C. Morgan , S. A. Ellison , H. M. Rose & D. H. Moore (1954). Structure and development of viruses observed in the electron microscope. II. Vaccinia and fowl pox viruses. J. exp. Med. 100, 301.

W. F. Noyes & B. K. Watson (1955). Studies on the increase of vaccine virus in cultured human cells by means of the fluorescent antibody technique. J. exp. Med. 102, 237.

R. W. G. Wyckoff (1951). The virus of vaccinia in chick embryo membrane. Proc. nat. Acad. Sci., Wash., 37, 565.

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Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
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