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    Pintar, K. D. M. Fazil, A. Pollari, F. Waltner-Toews, D. Charron, D. F. McEwen, S. A. and Walton, T. 2012. Considering the Risk of Infection by Cryptosporidium via Consumption of Municipally Treated Drinking Water from a Surface Water Source in a Southwestern Ontario Community. Risk Analysis, Vol. 32, Issue. 7, p. 1122.


    Robinson, Guy and Chalmers, Rachel M. 2012. Assessment of polymorphic genetic markers for multi-locus typing of Cryptosporidium parvum and Cryptosporidium hominis. Experimental Parasitology, Vol. 132, Issue. 2, p. 200.


    Pintar, K. D. M. Fazil, A. Pollari, F. Charron, D. F. Waltner-Toews, D. and McEwen, S. A. 2010. A Risk Assessment Model to Evaluate the Role of Fecal Contamination in Recreational Water on the Incidence of Cryptosporidiosis at the Community Level in Ontario. Risk Analysis, Vol. 30, Issue. 1, p. 49.


    Putignani, Lorenza and Menichella, Donato 2010. Global Distribution, Public Health and Clinical Impact of the Protozoan PathogenCryptosporidium. Interdisciplinary Perspectives on Infectious Diseases, Vol. 2010, p. 1.


    Silverlås, Charlotte Näslund, Katarina Björkman, Camilla and Mattsson, Jens G. 2010. Molecular characterisation of Cryptosporidium isolates from Swedish dairy cattle in relation to age, diarrhoea and region. Veterinary Parasitology, Vol. 169, Issue. 3-4, p. 289.


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Molecular typing of Cryptosporidium parvum associated with a diarrhoea outbreak identifies two sources of exposure

  • J. G. MATTSSON (a1), M. INSULANDER (a2), M. LEBBAD (a3), C. BJÖRKMAN (a4) and B. SVENUNGSSON (a2)
  • DOI: http://dx.doi.org/10.1017/S0950268807009673
  • Published online: 26 October 2007
Abstract
SUMMARY

An outbreak of cryptosporidiosis associated with exposure to outdoor swimming-pool water affected an estimated 800–1000 individuals. PCR products were obtained from faecal specimens from 30 individuals who tested positive for Cryptosporidium oocysts. RFLP and sequencing analyses showed that all individuals were infected with Cryptosporidium parvum. Among the infected individuals, five had just swum in an adjacent indoor pool during the same period, and had no identified contact with individuals linked to the outdoor pool. With the use of subgenotyping based on analysis of three mini- and microsatellite loci, MS1, TP14, and GP15, we could identify two sources of exposure. One subtype was associated with the outdoor pool and another with the indoor pool. These data demonstrate that the use of mini- and microsatellite loci as markers for molecular fingerprinting of C. parvum isolates are valuable in the epidemiological investigation of outbreaks.

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Corresponding author
*Author for correspondence: B. Svenungsson, M.D., Ph.D., Associate Professor, Department of Communicable Disease Control and Prevention, Karolinska University Hospital, Norrbacka, S-171 76 Stockholm, Sweden. (Email: bo.svenungsson@sll.se)
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11. M Mallon , Population structures and the role of genetic exchange in the zoonotic pathogen Cryptosporidium parvum. Journal of Molecular Evolution 2003; 56: 407417.

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14. WB Strong , J Gut , RG Nelson . Cloning and sequence analysis of a highly polymorphic Cryptosporidium parvum gene encoding a 60-kilodalton glycoprotein and characterization of its 15- and 45-kilodalton zoite surface antigen products. Infection and Immunity 2000; 68: 41174134.

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Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
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