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The relationship between the serogroup antigen and lipopolysaccharide of Legionella pneumophila

  • J. W. Conlan (a1) and L. A. E. Ashworth (a1)

Summary

Serogroup-specific antigen was extracted from a number of Legionella pneumophila strains and compared with phenol-water extracted lipopolysaccharide on the basis of gel filtration, chemical analysis, SDS-PAGE and reaction with serogroup-specific antibody in immunoblots. Serogroup specificity is apparently borne by the O side-chains of the lipopolysaccharide, which, although smooth in type, partitions in the phenol phase. For four serogroup 1 strains tested, there was no qualitative correlation between O side-chain length and pulmonary virulence for guinea-pigs.

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References

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Baskerville, A., Fitzgeorge, R. B., Conlan, J. W., Ashworth, L. A. E., Gibson, D. H. & Morgan, C. P. (1983). Studies on protective immunity to aerosol challenge with Legionella pneumophila. Zentralblatt für Bacteriologie, Mikrobiologie und Hygiene (Abteilung 1, Originate A) 255, 150155.
Collins, M. T., Espersen, F., Hoiby, N., Cho, S. N., Friis-Moller, A. & Reif, J. S. (1983). Crossed immunoelectrophoretic analysis of Legionella pneumophila serogroup 1 antigens. Infection and Immunity 35, 14281440.
Diedrich, D. L., Domenico, A. R. & Fralick, J. A. (1983). Influence of urea on the resolution of lipopolysaccharide in sodium dodecylsulfate polyacrylamide gels. Journal of Microbiological Methods 1, 245251.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28, 350356.
Edelstein, P. H. (1981). Improved semi-selective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. Journal of Clinical Microbiology 14, 298303.
Farshey, C. E., Klein, G. C. & Feely, J. C. (1978). Detection of antibodies to Legionnaires' disease organism by microagglutination and micro-enzyme-linked immunosorbent assay tests. Journal of Clinical Microbiology 7, 327331.
Fitzgeorge, R. B., Baskerville, A., Broster, M., Hambleton, P. & Dennis, P. J. (1983). Aerosol infection of animals with strains of Legionella pneumophila of different virulence: comparison with intraperitoneal and intranasal routes of infection. Journal of Hygiene 90, 8189.
Fitzgeorge, R. B. (1985). Effect of antibiotics on growth of L. pneumophila in guinea-pig alveolar phagocytes infected in vivo by aerosol. Journal of Infection 10, 189193.
Flesher, A. R., Jennings, H. J., Lugowski, C. & Kasper, D. L. (1982). Isolation of a serogroup 1 specific antigen from Legionella pneumophila. Journal of Infectious Diseases 145, 224233.
Gabay, J. E. & Horwitz, M. A. (1985). Isolation and characterisation of the cytoplasmic and outer membranes of Legionella pneumophila. Journal of Experimental Medicine 161, 409422.
Goldman, R. C. & Leive, L. (1980). Heterogeneity of antigenic side chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella LT2. European Journal of Biochemistry 107, 137143.
Harrison, T. G. & Taylor, A. G. (1982). A rapid microagglutination test for the diagnosis of Legionella pneumophila(serogroup 1) infection. Journal of Clinical Pathology 35, 10281031.
Irons, L. I., Ashworth, L. A. E. & Wilton-Smith, P. (1983). Heterogeneity of the filamentous haemagglutinin of Bordetella pertussis studied with monoclonal antibodies. Journal of General Microbiology 129, 27692778.
Johnson, W., Elliot, J. A., Helms, C. M. & Renner, E. D. (1979). A high molecular weight antigen in Legionnaires Disease Bacterium: Isolation and partial characterisation. Annals of Internal Medicine 90, 638641.
Joly, R. J. & Kenny, G. E. (1982). Antigenic analysis of Legionella pneumophila and Tatlockia micdadei by two-dimensional immunoelectrophoresis. Infection and Immunity 35, 721729.
Karkhanis, Y. D., Zeltner, J. Y., Jackson, J. J. & Carlo, D. J. (1978). A new improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of Gram negative bacteria. Analytical Biochemistry 85, 595601.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680685.
Medearis, D. M., Camita, B. M. & Heath, E. C. (1968). Cell wall composition and virulence in Escherichia coli. Journal of Experimental Medicine 128, 399414.
Nakane, P. K. & Kawaoi, A. (1974). Peroxidase-labelled antibody. A new method of conjugation. Journal of Histochemistry and Cytochemistry 22, 10841091.
Palva, E. T. & Makela, P. H. (1980). Lipopolysaccharide heterogeneity in Salmonella typhimurium analysed by sodium dodecylsulphate polyacrylamide gel electrophoresis. European Journal of Biochemistry 107, 137143.
Peppler, M. S. (1984). Two physically and serologically distinct lipopolysaccharide profiles in strains of Bordetella pertussis and their phenotype variants. Infection and Immunity 43, 224232.
Ristroph, J. D., Hedlund, K. W. & Allen, R. G. (1980). Liquid medium for growth of Legionella pneumophila. Journal of Clinical Microbiology 11, 1921.
Roantree, R. J. (1967). Salmonella O antigens and virulence. Annual Reviews of Microbiology 21, 443466.
Schramek, S., Kazar, J. & Bazovska, S. (1982). Lipid A in Legionella pneumophila. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (Abteilung 1, Originale A) 252, 401404.
Stanek, G., Hirschl, A., Lessky, E., Wewalka, F., Ruckdeschel, G. & Wewalka, G. (1983).Indirect immunofluorescent assay (IFA), microagglutination test (MA) and enzyme linked immunosorbent assay (ELISA) in diagnosis of legionellosis. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (Abteilung I, Originale A) 255, 108114.
Tsai, C. M. & Frasch, C. E. (1982). A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Analytical Biochemistry 119, 115119.
Westphal, O. & Jann, K. (1965). Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. Methods in Carbohydrate Chemistry, volume V (ed. Whistler, R. L.), pp. 8991.
Wong, K. H., Schalla, W. O., Arko, A. J., Bullard, J. C. & Feely, J. C. (1979). Immunochemical, serologic and immunologic properties of major antigens isolated from Legionnaires' disease bacterium. Annals of Internal Medicine 90, 634638.
Wong, K. H., Schall, W. O., Wong, M. C, McMaster, P. R. B., Feely, J. C. & Arko, R. J. (1982). Biological activities of antigens from Legionella pneumophila. Seminars in Infectious Diseases. IV: Bacterial Vaccines.

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