Skip to main content Accessibility help
×
×
Home

The serological relationship between Escherichia coli O157 and Yersinia enterocolitica O9 using sera from patients with brucellosis

  • H Chart (a1), O. A Okubadejo (a2) and B Rowe (a1)

Summary

Sera from ten patients with positive brucella serology were used to investigate antibody cross-reactions between the O-antigens of Escherichia coli 0157 and Yersinia enterocolitica 09. SDS-PAGE profiles of lipopolysaccharide (LPS), purified from strains of E. coli 0157 and Y. enterocolitica 09, were reacted with sera by immunoblotting. All ten sera contained antibodies which bound to the LPS of E. coli 0157, and five of these sera also contained antibodies which bound to the LPS of Y. enterocolitica 09. Absorption studies using these five cross–reacting sera indicated the existence of at least three epitopes exposed on the O–antigens of E. coli 0157 and Y. enterocolitica 09. One antigen binding site appeared to be exposed on the LPS of both organisms, while one epitope was exposed on the LPS of E. coli 0157 only, and another on the LPS of Y. enterocolitica 09 only.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      The serological relationship between Escherichia coli O157 and Yersinia enterocolitica O9 using sera from patients with brucellosis
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      The serological relationship between Escherichia coli O157 and Yersinia enterocolitica O9 using sera from patients with brucellosis
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      The serological relationship between Escherichia coli O157 and Yersinia enterocolitica O9 using sera from patients with brucellosis
      Available formats
      ×

Copyright

References

Hide All
1.Levin, M, Walters, MDS, Barratt, TM. Hemolytic uremic syndrome. Adv Pediatr Infect Dis 1989; 4: 5182.
2.Chart, H, Scotland, SM, Rowe, B. Serum antibodies to Escherichia coli serotype 0157:H7 in patients with hemolytic uremic syndrome. J Clin Microbiol 1989; 27: 285–90.
3.Chart, H, Scotland, SM, Smith, HR, Rowe, B. Antibodies to Escherichia coli 0157 in patients with haemorrhagic colitis and haemolytic uraemic syndrome. J Clin Pathol 1989; 42: 973–6.
4.Gilmour, A, Walker, SJ. Isolation and identification of Yersinia enterocolitica and the Yersinia enterocolitica-like bacteria. J Appl Bacteriol Symp Suppl 1988; 213–36S.
5.Corbel, MJ. Brucellosis: Epidemiology and prevalence worldwide. In: Young, EJ, Corbel, MJ, eds. Brucellosis: Clinical and laboratory aspects. Florida: CRC Press, 1989; 2540.
6.Perry, MB, MacLean, L, Griffith, DW. Structure of the O-chain of the phenol-phase soluble lipopolysaccharide of Escherichia coli 0157: H7. Biochem Cell Biol 1986; 64: 21–8.
7.Caroff, M, Bundle, DR, Perry, MP. Structure of the O-chain of the phenol-phase solubale cellular lipopolysaccharide of Yersinia enterocolitica serotype 0:9. Eur J Biochem 1984; 139: 195200.
8.Bundle, DR, Cherwonogrodzky, JW, Caroff, M, Perry, MB. The lipopolysaccharides of Brucella abortus and B. Melitensis. Ann Inst Pasteur Microbiol 1987; 138: 92–8.
9.Corbell, MJ. The serological relationship between Brucella spp., Yersinia enterocolitica serotype IX and Salmonella serotypes of Kauffmann-White group N. J Hyg 1975; 75: 151–71.
10.Lindberg, AA, Haeggman, S, Karlson, K, Carlson, HE, Mair, NS. Enzyme immunoassay of the antibody response to Brucella and Yersinia enterocolitica 09 infections in humans. J Hyg 1982; 88: 295307.
11.Notenboom, RH, Borezyk, A, Karmali, MA, Duncan, LMC. Clinical relevance of a serological cross-reaction between Escherichia coli 0157 and Brucella abortus. Lancet 1987; II: 745.
12.Sandulache, R, Marx, A. Immunological studies of a Yersinia enterocolitica 09 lipopoly-saccharide cross-reacting with Brucell a abortus and Vibrio cholerae extracts. Ann Microbiol Inst Pasteur 1978; 129: 425–35.
13.Stuart, FA, Corbel, MJ. Identification of a serological cross-reaction between Brucella abortus and Escherichia coli. Vet Rec 1982; 110: 202–3.
14.Chart, H, Cheasty, T, Cope, D, Gross, RJ, Rowe, B. The serological relationship between Yersinia enterocolitica 09 and Escherichia coli 0157 using sera from patients with yersiniosis and haemolytic uraemic syndrome. Epidemiol Infect. In press.
15.Parratt, D, Neilsen, KH, White, RG, Payne, DJH. Radioimmunoassay of IgM, IgG and IgA Brucella antibodies. Lancet 1977; I: 1076–8.
16.Hewitt, WG, Payne, DJH. Estimation of IgG and IgM Brucella antibodies in infected and non-infected persons by a radioimmune technique. J Clin Pathol 1984; 37: 693–6.
17.Westphal, O, Jann, K. Bacteriallipopolysaccharide: Extraction with phenol-water and further applications of the procedure. Methods Carbohydr Chem 1965; 5: 8391.
18.Chart, H, Rowe, B. Purification of lipopolysaccharide from strains of Yersinia enterocolitica belonging to serogroups 03 and O9. FEMS Microbiol 1991; 77: 341–6.
19.Wray, W, Boulikas, T, Wray, VP, Hancock, R. Silver staining of proteins in polyacrylamide gels. Anal Biochem 1981; 118: 197203.
20.Tsai, C-M, Frasch, CE. A sensitive silver stain for detecting lipopolysaccharide in polyacrylamide gels. Anal Biochem 1982; 119: 115–19.
21.Griffiths, E, Stevenson, P, Thorpe, R, Chart, H. Naturally occurring antibodies in human sera that react with the iron-regulated outer membrane proteins of Escherichia coli. Infect Immun 1985; 47: 808–13.
22.Chart, H, Smith, HR, Scotland, SM, Rowe, B, Milford, DV, Taylor, CM. Serological identification of Escherichia coli 0157: H7 infection in haemolytic uraemic syndrome. Lancet 1991; 337: 138–40.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed