Skip to main content
    • Aa
    • Aa
  • Get access
    Check if you have access via personal or institutional login
  • Cited by 128
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Sanford, B. Holinka, L.G. O'Donnell, V. Krug, P.W. Carlson, J. Alfano, M. Carrillo, C. Wu, Ping Lowe, Andre Risatti, G.R. Gladue, D.P. and Borca, M.V. 2016. Deletion of the thymidine kinase gene induces complete attenuation of the Georgia isolate of African swine fever virus. Virus Research, Vol. 213, p. 165.

    Kollias, Christina M. Huneke, Richard B. Wigdahl, Brian and Jennings, Stephen R. 2015. Animal models of herpes simplex virus immunity and pathogenesis. Journal of NeuroVirology, Vol. 21, Issue. 1, p. 8.

    Peters, Cole and Rabkin, Samuel D 2015. Designing herpes viruses as oncolytics. Molecular Therapy — Oncolytics, Vol. 2, p. 15010.

    Terrell, S. L. Pesola, J. M. and Coen, D. M. 2014. Roles of conserved residues within the pre-NH2-terminal domain of herpes simplex virus 1 DNA polymerase in replication and latency in mice. Journal of General Virology, Vol. 95, Issue. Pt_4, p. 940.

    Ishioka, Ken Ikuta, Kazufumi Sato, Yuka Kaneko, Hisatoshi Sorimachi, Kotaro Fukushima, Eiko Saijo, Masayuki and Suzutani, Tatsuo 2013. Herpes simplex virus type 1 virion-derived US11 inhibits type 1 interferon-induced protein kinase R phosphorylation. Microbiology and Immunology, Vol. 57, Issue. 6, p. 426.

    Racz, Rebecca Chung, Monica Xiang, Zuoshuang and He, Yongqun 2013. Systematic annotation and analysis of “virmugens”—Virulence factors whose mutants can be used as live attenuated vaccines. Vaccine, Vol. 31, Issue. 5, p. 797.

    Arnizaut, AB and Hanson, LA 2011. Antibody response of channel catfish after channel catfish virus infection and following dexamethasone treatment. Diseases of Aquatic Organisms, Vol. 95, Issue. 3, p. 189.

    Azab, Walid Kato, Kentaro Abdel-Gawad, Azza Tohya, Yukinobu and Akashi, Hiroomi 2011. Equine herpesvirus 4: Recent advances using BAC technology. Veterinary Microbiology, Vol. 150, Issue. 1-2, p. 1.

    Biswas, Subhajit and Field, Hugh J. 2011. Antiviral Drug Strategies.

    Field, Hugh J. and Biswas, Subhajit 2011. Antiviral drug resistance and helicase–primase inhibitors of herpes simplex virus. Drug Resistance Updates, Vol. 14, Issue. 1, p. 45.

    Thompson, Richard L. and Sawtell, Nancy M. 2011. The herpes simplex virus type 1 latency associated transcript locus is required for the maintenance of reactivation competent latent infections. Journal of NeuroVirology, Vol. 17, Issue. 6, p. 552.

    Vere Hodge, Anthony and Field, Hugh J. 2011. Genetics and Evolution of Infectious Disease.

    Azab, Walid Tsujimura, Koji Kato, Kentaro Arii, Jun Morimoto, Tomomi Kawaguchi, Yasushi Tohya, Yukinobu Matsumura, Tomio and Akashi, Hiroomi 2010. Characterization of a thymidine kinase-deficient mutant of equine herpesvirus 4 and in vitro susceptibility of the virus to antiviral agents. Antiviral Research, Vol. 85, Issue. 2, p. 389.

    Biswas, S. Miguel, R. N. Sukla, S. and Field, H. J. 2009. A mutation in helicase motif IV of herpes simplex virus type 1 UL5 that results in reduced growth in vitro and lower virulence in a murine infection model is related to the predicted helicase structure. Journal of General Virology, Vol. 90, Issue. 8, p. 1937.

    Kelly, Barbara J. Fraefel, Cornel Cunningham, Anthony L. and Diefenbach, Russell J. 2009. Functional roles of the tegument proteins of herpes simplex virus type 1. Virus Research, Vol. 145, Issue. 2, p. 173.

    Biswas, Subhajit Tiley, Laurence S. Zimmermann, Holger Birkmann, Alexander and Field, Hugh J. 2008. Mutations close to functional motif IV in HSV-1 UL5 helicase that confer resistance to HSV helicase–primase inhibitors, variously affect virus growth rate and pathogenicity. Antiviral Research, Vol. 80, Issue. 1, p. 81.

    Shen, Y and Nemunaitis, J 2006. Herpes simplex virus 1 (HSV-1) for cancer treatment. Cancer Gene Therapy, Vol. 13, Issue. 11, p. 975.

    Brandt, Curtis R. 2005. The role of viral and host genes in corneal infection with herpes simplex virus type 1. Experimental Eye Research, Vol. 80, Issue. 5, p. 607.

    Brandt, Curtis 2004. Virulence genes in herpes simplex virus type 1 corneal infection. Current Eye Research, Vol. 29, Issue. 2-3, p. 103.

    Lachmann, Robin 2004. Herpes simplex virus-based vectors. International Journal of Experimental Pathology, Vol. 85, Issue. 4, p. 177.


The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice

  • H. J. Field (a1) and P. Wildy (a1)
  • DOI:
  • Published online: 01 May 2009

The pathogenicity for mice of two mutants of herpes simplex virus (type 1 and type 2), which fail to induce thymidine kinase, were compared with their respective parent strains. The mutants were much less virulent than the parents following either intracerebral or peripheral inoculation. The replication of the virus at the site of inoculation and its progression into the nervous system were studied. Following a very large inoculum in the ear, the type 1 mutant was found to establish a latent infection in the cervical dorsal root ganglia. Mice inoculated intracerebrally with small doses of the mutant viruses were solidly immune to challenge with lethal doses of the parent strain.

Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

H. J. Field & T. J. Hill (1974). The pathogenesis of pseudorabies in mice following peripheral inoculation. Journal of General Virology 23, 145–57.

T. J. Hill , H. J. Field & W. A. Blyth (1975). Acute and recurrent infection with herpes simplex virus in the mouse: a model for studying latency and recurrent disease. Journal of General Virology 28, 341–53.

A. T. Jamieson , G. A. Gentry & J. H. Subak-Sharpe (1974). Induction of both thymidine and deoxycytidine kinase activity by herpes viruses. Journal of General Virology 24, 465–80.

M. E. Thouless (1972). Serological properties ofthymidine kinase produced in cells infected with type 1 or type 2 herpes virus. Journal of General Virology 17, 307–15.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
Please enter your name
Please enter a valid email address
Who would you like to send this to? *