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Use of plasmid profile typing for surveillance of Salmonella enteritidis phage type 4 from humans, poultry and eggs

  • E. J. Threlfall (a1), M. D. Hampton (a1), H. Chart (a1) and B. Rowe (a1)

Summary

Plasmids were found in 1022 of 1089 (94%) of drug–sensitive strains of Salmonella enteritidis phage type 4 from humans (sporadic and outbreak cases), poultry (chickens) and eggs in England and Wales in the 5-year period 1988–92 and 25 plasmid profile patterns were identified. Strains characterized by a single plasmid of 38 MDa predominated ( = plasmid profile type SE 38), comprising over 90% of isolates from humans, 70% from poultry and 92% from eggs. Eleven profile types were identified in strains from humans, 21 in strains from poultry and 3 in strains from eggs. Eight of the 11 patterns identified in human isolates were found in strains from poultry and 2 in strains from eggs. In contrast 15 patterns seen in poultry were not found in strains from humans. Four percent of strains from humans and 13% from poultry did not carry the 38 MDa plasmid but all strains from eggs were found to carry this plasmid. The second most common profile type in strains isolated between 1981 and 1988 was not identified in strains isolated from 1988–92. It is concluded that plasmid profile typing is a useful method for rapid differentiation within phage type 4 of S. enteritidis but that methods which can discriminate within the predominant profile type, SE 38, are now required.

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Copyright

References

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Anonymous. The microbiological safety of food. Pt 1. London: HMSO. 1990.
Ward, LR, de Sa, JDH, Rowe, B. A phage-typing scheme for Salmonella enteritidis. Epidemiol Infect 1987; 99: 291304.
Anonymous. PHLS-SVS: update on salmonella infection. Public Health Laboratory Service-State Veterinary Service. 1993: 14.
Anonymous. PHLS-SVS: update on salmonella infection. Public Health Laboratory Service-State Veterinary Service. 1993: 15.
Humphrey, TJ, Mead, GC, Rowe, B. Poultry meat as a source of human salmonellosis in England and Wales. Epidemiol Infect 1988: 100: 175–84.
Coyle, EF, Palmer, SR,Ribeiro, CD, Jones, HI, Howard, AJ, Ward, LR, Rowe, B. Salmonella enteritidis phage type 4: association with hens' eggs. Lancet 1988; ii: 1295–7.
Threlfall, EJ, Ward, LR, Rowe, B. Subdivision of Salmonella enteritidis by plasmid profile typing. Epidemiol Infect 1989: 102: 459–65.
Anderson, ES, Threlfall, EJ. The characterization of plasmids in the enterobacteria. J Hyg 1974: 72: 471–87.
Ward, LR, Threlfall, EJ, Rowe, B. Multiple drug resistance in salmonellae in England and Wales: a comparison between 1981 and 1988. J Clin Pathol 1990: 43: 563–6.
Kado, CL, Liu, S-T. Procedure for detection for large and small plasmids. J Bact 1981; 145: 1365–73.
Threlfall, EJ, Rowe, B, Ferguson, JL, Ward, LR. Characterization of plasmids conferring resistance to gentamicin and apramycin in strains of Salmonella typhimurium phage type 204c isolated in Britain. J Hyg 1986: 97: 419–26.
Macrina, FL, Kopecko, DJ, Jones, KR, Ayers, DJ, McCowan, SM. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmids. Plasmid 1978: 1: 417–20.
Helmuth, R, Stephan, R, Bunge, C, Hoog, B, Steinbeck, A, Bulling, E. Epidemiology of virulence-associated plasmids and outer membrane protein patterns within seven common Salmonella serovars. Infect Immunity 1985: 48: 175–82.
Lam, S, Roth, JR. IS200: a Salmonella-specific insertion sequence. Cell 1983: 34: 951–60.
Stanley, J, Jones, CS, Threlfall, EJ. Evolutionary lines among Salmonella enteritidis phage types are identified by insertion sequence IS200 distribution. FEMS Microbiol Lett 1991; 82: 8390.
Stanley, J, Goldsworthy, M, Threlfall, EJ. Molecular phylogenetie typing of pandemic isolates of Salmonella enteritidis. FEMS Microbiol Lett 1992; 90: 153–60.
Stanley, J, Burens, AP, Threlfall, EJ, Chowdrey, N, Goldsworthy, M. Genetic relationships among strains of Salmonella enteritidis in a national epidemic in Switzerland. Epidemiol Infect 1992; 108: 213–20.
Brown, DJ, Threlfall, EJ, Hampton, MD, Rowe, B. Molecular characterization of plasmids in Salmonella enteritidis phage types. Epidemiol Infect 1993; 110: 209–16.
Chart, H, Threlfall, EJ, Rowe, B. Virulence of Salmonella enteritidis phage type 4 is related to the possession of a 38 MDa plasmid. FEMS Microbiol Lett 1989; 58: 299304.
Buchmeier, NA, Heffron, F. Inhibition of macrophage phagosome-lysosome fusion by Salmonella typhimurium. Infect Immunity 1991; 59: 2232–8.
Montenegro, MA, Morelli, G, Helmuth, R. Heteroduplex analysis of Salmonella virulence plasmids and their prevalence in isolates different sources. Microbiol Pathogenesis 1991; 6; 391–7.
Humphrey, TJ, Baskerville, A, Mawer, S, Rowe, B, Hopper, S. Salmonella enteritidis phage type 4 from the contents of intact eggs: a study involving naturally-infected hens. Epidemiol Infect 1989: 103: 415–23.

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