Skip to main content
    • Aa
    • Aa

Virulence properties of Escherichia coli strains belonging to serogroups O26, O55, O111 and O128 isolated in the United Kingdom in 1991 from patients with diarrhoea

  • S. M. Scotland (a1), G. A. Willshaw (a1), H. R. Smith (a1), B. Said (a1), N. Stokes (a1) and B. Rowe (a1)...

Some strains of Escherichia coli belonging to serogroups O26, O55, O111 or O128 produce Vero cytotoxin (VT). These serogroups are included in the range of enteropathogenic E. coli (EPEC) serogroups for which commercial antisera are available. In an attempt to obtain information on VT-producing strains other than those of serogroup O157, 122 strains belonging to these four serogroups and isolated in 1991 from patients with diarrhoea in the United Kingdom were tested for hybridization with VT probes. Only 18 of the 122 strains were VT-positive and these were O26 or O128. However 90 strains hybridized with the E. coli attaching and effacing (eae) probe (including 14 VT-positive strains) and 17 with the enteroaggregative E. coli (EAggEC) probe. For 78 eae-positive and 9 EAggEC-positive strains, tissue culture tests correlated with the probe results as the strains gave, respectively, either localized adhesion and a positive fluorescent-actin staining test or a characteristic aggregative attachment. A total of 111 of the 122 strains belonging to serogroups O26, O55, O111 or O128 possessed properties that may be associated with the ability to cause human diarrhoeal disease, and similar studies are needed on strains from the other classical EPEC serogroups.

Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

1. MA Karmali . Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev 1989; 2: 1538.

2. HR Smith , SM Scotland . Vero cytotoxin-producing strains of Escherichia coli. J Med Microbiol 1988; 26: 7785.

3. LW Riley . The epidemiologic, clinical, and microbiologic features of hemorrhagic colitis. Ann Rev Microbiol 1987; 41: 383407.

5. M Bitzan , H Karch , MG Maas , Clinical and genetic aspects of shiga-like toxin production in traditional enteropathogenic Escherichia coli. Zentralbl Bakteriol 1991; 274: 496506.

7. M Katouli , I Kühn , R Möllby . Biochemical phenotypes of enteropathogenic Escherichia coli common to Iran and Sweden. J Med Microbiol 1991; 35: 270–7.

9. SM Scotland , NP Day , B Rowe . Production of a cytotoxin affecting Vero cells by strains of Escherichia coli belonging to traditional enteropathogenic serogroups. FEMS Microbiol Lett 1980; 7: 15–7.

10. SM Scotland , GA Willshaw , HR Smith , B Rowe . Properties of strains of Escherichia coli O26: H11 in relation to their enteropathogenic or enterohemorrhagic classification. J Infect Dis 1990; 162: 1069–74.

11. GA Willshaw , SM Scotland , HR Smith , B Rowe . Properties of Vero cytotoxin-producing Escherichia coli of human origin belonging to O-serogroups other than O157. J Infect Dis 1992; 166: 797802.

14. A Thomas , HR Smith , GA Willshaw , B Rowe . Non-radioactively labelled polynucleotide and oligonucleotide DNA probes, for selectively detecting Escherichia coli strains producing Vero cytotoxins VT1, VT2 and VT2 variant. Molec Cell Probes 1991; 5: 129–35.

15. MM Levine , J-g Xu , JB Kaper , A DNA probe to identify enterohemorrhagic Escherichia coli of O157:H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome. J Infect Dis 1987; 156: 175–82.

17. AE Jerse , J Yu , BD Tall , JB Kaper . A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Natl Acad Sci USA 1990; 87: 7839–43.

18. B Baudry , SJ Savarino , P Vial , JB Kaper , MM Levine . A sensitive and specific DNA probe to identify enteroaggregative Escherichia coli, a recently discovered diarrheal pathogen. J Infect Dis 1990; 161: 1249–51.

22. SM Scotland , GA Willshaw , HR Smith , RJ Gross , B Rowe . Adhesion to cells in culture and plasmid profiles of enteropathogenic Escherichia coli isolated from outbreaks and sporadic cases of infant diarrhoea. J Infect 1989; 19: 237–49.

24. GA Willshaw , HR Smith , MM McConnell , Plasmid-encoded production of coli-surface associated antigen 1 (CS1) in a strain of Escherichia coli serotype O139. H28. Microb Path 1990; 9: 111.

33. HR Smith , SM Scotland , N Stokes , B Rowe . Examination of strains belonging to enteropathogenic Escherichia coli serogroups for genes encoding EPEC adherence factor and Vero cytotoxins. J Med Microbiol 1990; 31: 235–40.

35. SM Scotland , HR Smith , B Said , GA Willshaw , T Cheasty , B Rowe . Identification of enteropathogenic Escherichia coli isolated in Britain as enteroaggregative or as members of a subclass of attaching-and-effacing E. coli not hybridising with the EPEC adherence-factor probe. J Med Microbiol 1991; 35: 278–83.

38. MK Bhan , P Raj , MM Levine , Enteroaggregative Escherichia coli associated with persistent diarrhea in a cohort of rural children in India. J Infect Dis 1989; 159: 1061–4.

39. A Cravioto , A Tello , A Navarro , Association of Escherichia coli HEp-2 adherence patterns with type and duration of diarrhoea. Lancet 1991; 337: 262–4.

41. KJ Morris , GG Rao . Conventional screening for enteropathogenic Escherichia coli in the UK. Is it appropriate or necessary? J Hosp Infect 1992; 21: 163–7.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Epidemiology & Infection
  • ISSN: 0950-2688
  • EISSN: 1469-4409
  • URL: /core/journals/epidemiology-and-infection
Please enter your name
Please enter a valid email address
Who would you like to send this to? *


Full text views

Total number of HTML views: 0
Total number of PDF views: 1 *
Loading metrics...

Abstract views

Total abstract views: 20 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 19th September 2017. This data will be updated every 24 hours.