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    Tirupataiah, Sirupangi Jamir, Imlitoshi Srividya, Indukuri and Mishra, Krishnaveni 2014. Yeast Nkp2 is required for accurate chromosome segregation and interacts with several components of the central kinetochore. Molecular Biology Reports, Vol. 41, Issue. 2, p. 787.


    Barbera, M. A. and Petes, T. D. 2006. Selection and analysis of spontaneous reciprocal mitotic cross-overs in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences, Vol. 103, Issue. 34, p. 12819.


    Clarke, Louise and Carbon, John 1983. Genomic substitutions of centromeres in Saccharomyces cerevisiae. Nature, Vol. 305, Issue. 5929, p. 23.


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Genetic analysis of spontaneous half-sectored colonies of Saccharomyces cerevisiae

  • J. R. Johnston (a1)
  • DOI: http://dx.doi.org/10.1017/S0016672300012581
  • Published online: 01 April 2009
Abstract
SUMMARY

Genetic analysis of spontaneous half-sectored colonies of a diploid strain homozygous for ade2 and heterozygous for linked markers aro1A, hom2, ade8 and trp4 was undertaken. Some of these were due to an unexpectedly high rate of ade2 reversion and others to a low incidence of haploid sectors. About 50% resulted from mitotic recombination. Only a few were due to mitotic crossing over alone, the majority resulted from non-reciprocal ‘conversion’, including recombination extending over two loosely-linked loci, and some colonies featured both mitotic crossing over and mitotic conversion. The latter indicate that negative interference operates for mitotic recombination. The rate of mitotic recombination for ade8, calculated from the frequency of half-sectored colonies was 3·1 × 10−4 per cell division.

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G. Pontecorvo & E. Käfer (1958). Genetic analysis based on mitotic recombination. Advances in Genetics 9, 71104.

H. Roman & F. Jacob (1958). A comparison of spontaneous and ultraviolet-induced allelic recombination with reference to the recombination of outside markers. Cold Spring Harbor Symposia on Quantitative Biology 23, 155160.

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Genetics Research
  • ISSN: 0016-6723
  • EISSN: 1469-5073
  • URL: /core/journals/genetics-research
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