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Pleiotropy of p-fluorophenylalanine-resistant and antibiotic hypersensitive mutants of Pseudomonas aeruginosa

  • N. W. Dunn (a1) and B. W. Holloway (a1)

Summary

Mutants of Pseudomonas aeruginosa isolated as being resistant to p-fluorophenylalanine (FPA-r) are pleiotropic, showing patterns of phenotypic alteration of host-controlled modification, growth rate at 37 and 43 °C, ability to grow on minimal medium, response to antibiotics and ribosomal characteristics. Alterations in host-controlled modification are also found in FPA-r mutants of Escherichia coli B.

The pleiotropy arising in these FPA-r mutants of P. aeruginosa appears to result from alterations in the specificity of protein synthesis. Phenotypic variations in host-controlled modification of the type found in the FPA-r mutants may provide a method for the detection of suppressor mutations which act by miscoding.

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References

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Apirion, D. (1966). Altered ribosomes in a suppressor strain of Escherichia coli. Journal of Molecular Biology 16, 285301.
Apirion, D. (1967). Three genes that affect Escherichia coli ribosomes. Journal of Molecular Biology 30, 255275.
Bruno, G. A. & Christian, J. E. (1961). Determination of carbon-14 in aqueous bicarbonate solutions by liquid scintillation counting techniques. Analytical Chemistry 33, 12161218.
Davies, J. (1966). Streptomycin and the genetic code. Cold Spring Harbor Symposium on Quantitative Biology 31, 665670.
Dussoix, D. & Arber, W. (1962). Host specificity of DNA produced by Escherichia coli. II. Control over acceptance of DNA from infecting phage λ. Journal of Molecular Biology 5, 3749.
von Ehrenstein, G. & Lipmann, F. (1961). Experiments on haemoglorin synthesis. Proceedings of the National Academy of Sciences, U.S.A. 47, 941950.
Fangman, W. L. & Neidhardt, F. C. (1964). Demonstration of an altered amino-acyl ribonucleic acid synthetase in a mutant of Escherichia coli. Journal of Biological Chemistry 239, 18391843.
Fargie, B. & Holloway, B. W. (1965). Absence of clustering of functionally related genes in Pseudomonas aeruginosa. Genetical Research 6, 284299.
Gartner, T. K. & Orias, E. (1966). Effects of mutation to streptomycin resistance on the rate of translocation of mutant genetic information. Journal of Bacteriology 91, 10211028.
Gartner, T. K., Orias, E., Lennan, J. E., Beeson, J. & Reid, P. J. (1969). The molecular basis of suppression in an ochre suppressor strain possessing altered ribosomes. Proceedings of the National Academy of Sciences, U.S.A. 62, 946951.
Holloway, B. W. (1965). Variations in restriction and modification of bacteriophage following increase in growth temperature of Pseudomonas aeruginosa. Virology 25, 634642.
Holloway, B. W. (1969). Genetics of Pseudomonas. Bacteriological Reviews 33, 419443.
Holloway, B. W., Egan, J. B. & Monk, M. (1960). Lysogeny in Pseudomonas aeruginosa. Australian Journal of Experimental Biology and Medical Science 38, 321330.
Holloway, B. W. & Fargie, B. (1960). Fertility factors and genetic linkage in Pseudomonas aeruginosa. Journal of Bacteriology 80, 362368.
Holloway, B. W., Khishnapillai, V. & Stanisich, V. (1971). Pseudomonas genetics. Annual Review of Genetics (in the Press).
Holloway, B. W. & van de Putte, P. (1968). Lysogeny and bacterial recombination. In Replication and Recombination of Genetical Material (ed. Peacock, W. J. and Brock, R. D.), pp. 175183. Australian Academy of Science.
Isaac, J. & Holloway, B. W. (1968). Control of pyrimidine biosynthesis in Pseudomonas aeruginosa. Journal of Bacteriology 96, 17321741.
Kuwano, M., Ishikawa, M. & Endo, H. (1968). Su-II-specific restriction of amber suppression by mutation to streptomycin resistance. Journal of Molecular Biology 33, 513516.
Lederberg, S. (1957). Suppression of the multiplication of heterologous bacteriophages in lysogenic bacteria. Virology 3, 496513.
Lederberg, E. M., Cavalli-Sforza, L. & Lederberg, J. (1964). Interaction of streptomycin and a suppressor for galactose fermentation in E. coli K12. Proceedings of the National Academy of Sciences, U.S.A. 51, 678682.
Lipmann, F. (1969). Polypeptide chain elongation in protein biosynthesis. Science, New York 164, 10241031.
Nishizuka, Y. & Lipmann, F. (1966). Comparison of guanisine triphosphate split and polypeptide synthesis with a purified E. coli system. Proceedings of the National Academy of Sciences, U.S.A. 55, 212219.
Otsuji, N. & Aono, H. (1968). Effect of mutation to streptomycin resistance on amber suppressor genes. Journal of Bacteriology 96, 4350.
Rolfe, B. & Holloway, B. W. (1968). Genetic control of DNA specificity in Pseudomonas aeruginosa. Genetical Research, Cambridge 12, 99102.
Stanisich, V. & Holloway, B. W. (1969). Conjugation in Pseudomonas aeruginosa. Genetics 61, 327339.
Waltho, J. A. & Holloway, B. W. (1966). Suppression of fluorophenylalanine resistance by mutation to streptomycin resistance in Pseudomonas aeruginosa. Journal of Bacteriology 92, 3542.
Waltho, J. A. (1968). Genetic aspects of enzyme regulation in Pseudomonas aeruginosa. Ph.D. thesis, University of Melbourne.
Wood, W. B. (1966). Host specificity of DNA produced by Escherichia coli; bacterial mutations affecting restriction and modification of DNA. Journal of Molecular Biology 16, 118133.

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