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Resistance of Escherichia coli to penicillins: II. An improved mapping of the ampA gene

  • Kerstin G. Eriksson-Grennberg (a1)

Extract

The first mutation step towards ampicillin resistance in Escherichia coli occurs in the ampA gene, and gives resistance to a D, L-ampicillin concentration of 10 μg/ml. Using interrupted conjugation and transduction experiments ampA was found to be located at 82 min on the time scale of Taylor & Thoman (1964). A number of adjacent markers were studied and the probable gene order of the ampA region was found to be argH-metA-uvrA-ampA-purA-fdp-pyrB. Two independent alleles of ampA were cotransduced with fdp+ and purA+. The phenotypic expression of ampA in a purA strain has been investigated.

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References

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