Skip to main content
×
×
Home

Genetic variability in Indian populations of banana corm weevil [Cosmopolites sordidus (Coleoptera: Curculionidae)] assessed by RAPDs and AFLPs

  • Sweta Kumari U. Yadav (a1), Jyotsna Singh (a1), B. Padmanaban (a2) and Lalitha Sunil Kumar (a1)
Abstract

Cosmopolites sordidus (Germar), commonly known as banana corm weevil, is an important economic pest in Asia that can cause severe yield loss depending upon the stage at which infestation occurs. In spite of its economic importance, little is known about the population structure of this pest in India. Random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) were used to characterize the population genetic structure of C. sordidus collected from five hot spot locations in India. Nineteen RAPD primers and five selective AFLP primer combinations generated 147 and 304 amplification products, respectively. UPGMA dendrograms generated with both marker systems failed to reveal populations clustered based on geographic distance, which was confirmed by the Mantel test, which did not show a strong correlation between genetic distance and geographic distance. Values of indices of genetic identity showed that the populations were closely related. Though the gene flow estimate (Nm) between the populations was 0.469, suggesting restricted gene flow, the populations are not genetically distinct. These observations suggest that the range expansion of this banana pest in India has taken place through transport of infested corms and plant material, resulting in genetically close populations that are geographically distinct. These results provide important information on the population structure of this pest in India, which will aid in designing suitable strategies for its control and management, especially with respect to insecticide resistance.

Copyright
Corresponding author
*E-mail: ls.kumar@ncl.res.in; sklalitha2012@gmail.com
References
Hide All
Anderson, J. A., Churchill, G. A., Autrique, J. E., Tanksley, S. D. and Sorrells, M. E. (1993) Optimizing parental selection for genetic linkage maps. Genome 36, 181186.
Archak, S., Gaikwad, A. B., Gautam, D., Rao, E. V. V. B., Swamy, K. R. M. and Karihaloo, J. L. (2003) Comparative assessment of DNA fingerprinting techniques (RAPD, ISSR and AFLP) for genetic analysis of cashew (Anacardium occidentale L.) accessions of India. Genome 46, 362369.
Aylor, D. E. and Irwin, M. E. (1999) Aerial dispersal of pests and pathogens: implications for integrated pest management. Agricultural and Forest Meteorology 97, 233234.
Behura, S. K. (2006) Molecular marker systems in insects: current trends and future avenues. Molecular Ecology 15, 30873113. doi:10.1111/j.1365-294X.2006.03014.x.
Black, W. C. (1993) PCR with arbitrary primers: approach with care. Insect Molecular Biology 2, 16. doi:10.1111/j.1365-2583.1993.tb00118.x
Byrne, D. N. (1999) Migration and dispersal by the sweet potato whitefly, Bemisia tabaci . Agricultural and Forest Meteorology 97, 309316.
Castiglioni, L. and de Campos Bicudo, H. E. (2005) Molecular characterization and relatedness of Haematobia irritans (horn fly) populations, by RAPD-PCR. Genetica 124, 1121.
Cervera, M. T., Cabezas, J. A., Simón, B., Martinez-Zapater, J. M., Beitia, F. J. and Cenis, J. L. (2000) Genetic relationships among biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae) based on AFLP analysis. Bulletin of Entomological Research 90, 391396. doi: 10.1017/S0007485300000523.
Clark, P. L., Molina-Ochoa, J., Martinelli, S., Skoda, S. R., Isenhour, D. J., Lee, D. J., Krumm, J. T. and Foster, J. E. (2007) Population variation of the fall armyworm, Spodoptera frugiperda, in the Western Hemisphere. Journal of Insect Science 7, 110.
Collins, P. J., Treverrow, N. L. and Lambkin, T. M. (1991) Organophosphorous insecticide resistance and its management in the banana weevil borer, Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae), in Australia. Crop Protection 10, 215221.
de Graaf, J. (2006) Integrated pest management of the banana weevil, Cosmopolites sordidus (Germar), in South Africa. PhD thesis (Entomology). University of Pretoria, Pretoria.
Dellaporta, S. L., Wood, J. and Hicks, J. B. (1983) A plant DNA minipreparation, version II. Plant Molecular Biology Reporter 1, 1921.
Dvorak, V., Votypka, J., Aytekin, A. M., Alten, B. and Volf, P. (2011) Intraspecific variability of natural populations of Phlebotomus sergenti, the main vector of Leishmania tropica . Journal of Vector Ecology 36 (Supplement 1), S49S57. doi: 10.1111/j.1948-7134.2011.00111.x.
Gadelhak, G. G. and Enan, M. R. (2005) Genetic diversity among populations of red palm weevil, Rhynchophorus ferrugineus Olivier (Coleoptera: Curculionidae), determined by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). International Journal of Agriculture & Biology 7, 395399.
Godonou, I., Green, K. R., Oduro, K. A., Lomer, C. J. and Afreh-Nuamah, K. (2000) Field evaluation of selected formulations of Beauveria bassiana for the management of the banana weevil (Cosmopolites sordidus) on plantain (Musa sp., AAB group). Biocontrol Science and Technology 10, 779788.
Gold, C. S. and Messiaen, S. (2000) The banana weevil Cosmopolites sordidus. Musa Pest Fact Sheet No. 4. INIBAP, France. Available at: http://bioversityinternational.org/uploads/tx_news/The_banana_weevil_Cosmopolites_sordidus_696.pdf.
Gold, C. S., Bagabe, M. I. and Sendege, R. (1999a) Banana weevil, Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae): tests for suspected resistance to carbofuran and dieldrin in the Masaka District, Uganda. African Entomology 7, 189196.
Gold, C. S., Kagezi, G. H., Night, G. and Ragama, P. E. (2004) The effects of banana weevil, Cosmopolites sordidus, damage on highland banana growth, yield and stand duration in Uganda. Annals of Applied Biology 145, 263269.
Gold, C. S., Rukazambuga, N. D. T. M., Karamura, E. B., Nemeye, P. and Night, G. (1999b) Recent advances in banana weevil biology, population dynamics and pest status with emphasis on East Africa, pp. 3350. In Mobilizing IPM for Sustainable Banana Production in Africa. (edited by Frison, E., Gold, C. S., Karamura, E. B. and Sikora, R. A.). INIBAP, Montpellier, France. Available at: http://pdf.usaid.gov/pdf_docs/PNACK080.pdf.
Gold, C. S., Pena, J. E. and Karamura, E. B. (2001) Biology and integrated pest management for the banana weevil Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae). Integrated Pest Management Reviews 6, 79155.
Guasmi, F., Elfalleh, W., Hannachi, H., Fères, K., Touil, L., Marzougui, N., Triki, T. and Ferchichi, A. (2012) The use of ISSR and RAPD markers for genetic diversity among south Tunisian barley. ISRN Agronomy 2012, Article ID 952196, 10 pp.
Karam, N., Guglielmino, C. R., Bertin, S., Gomulski, L. M., Bonomi, A., Baldacchino, F., Simeone, V. and Malacrida, A. R. (2008) RAPD analysis in the parasitoid wasp Psyttalia concolor reveals Mediterranean population structure and provides SCAR markers. Biological Control 47, 2227.
Katiyar, S. K., Chandel, G., Tan, Y., Zhang, Y., Huang, B., Nugaliyadde, L., Fernando, K., Bentur, J. S., Inthavong, S., Constantino, S. and Bennett, J. (2000) Biodiversity of Asian rice gall midge (Orseolia oryzae Wood Mason) from five countries examined by AFLP analysis. Genome 43, 322332.
Kazachkova, N., Meijer, J. and Ekbom, B. (2008) Genetic diversity in European pollen beetle, Meligethes aeneus (Coleoptera: Nitidulidae), populations assessed using AFLP analysis. European Journal of Entomology 105, 807814.
Kiggundu, A., Pillay, M., Viljoen, A., Gold, C., Tushemereirwe, W. and Kunert, K. (2003) Enhancing banana weevil (Cosmopolites sordidus) resistance by plant genetic modification: A perspective. African Journal of Biotechnology 2, 563569.
Kim, K. S. and Sappington, T. W. (2004) Genetic structuring of boll weevil populations in the US based on RAPD markers. Insect Molecular Biology 13, 293303.
Krumm, J. T., Hunt, T. E., Skoda, S. R., Hein, G. L., Lee, D. J., Clark, P. L. and Foster, J. E. (2008) Genetic variability of the European corn borer, Ostrinia nubilalis, suggests gene flow between populations in the Midwestern United States. Journal of Insect Science 8, 72.
Kumar, L. S., Sawant, A. S., Gupta, V. S. and Ranjekar, P. K. (2001a) Comparative analysis of genetic diversity among Indian populations of Scirpophaga incertulas by ISSR-PCR and RAPD-PCR. Biochemical Genetics 39, 297309.
Kumar, L. S., Sawant, A. S., Gupta, V. S. and Ranjekar, P. K. (2001b) Genetic variation in Indian populations of Scirpophaga incertulas as revealed by RAPD-PCR analysis. Biochemical Genetics 39, 4357.
Labbe, P., Lenormand, T. and Raymond, M. (2005) On the worldwide spread of an insecticide resistance gene: a role for local selection. Journal of Evolutionary Biology 18, 14711484.
Lall, G. K., Darby, A. C., Nystedt, B., MacLeod, E. T., Bishop, R. P. and Welburn, S. C. (2010) Amplified fragment length polymorphism (AFLP) analysis of closely related wild and captive tsetse fly (Glossina morsitans morsitans) populations. Parasites & Vectors 3, 47. doi: 10.1186/1756-3305-3-47.
Lopes-da-Silva, M. and Vieira, L. G. E. (2007) Analysis of the genetic diversity in Metopolophium dirhodum (Walker) (Hemiptera, Aphididae) by RAPD markers. Revista Brasileira de Entomologia 51, 5457.
Loxdale, H. D., Hardie, J., Halbert, S., Foottit, R., Kidd, N. A. C. and Carter, C. I. (1993) The relative importance of short- and long-range movement of flying aphids. Biological Reviews 68, 291311. doi:10.1111/j.1469-185X.1993.tb00998.x.
Magaña, C., Beroiz Ramirez, B., Hernández-Crespo, P., Montes de Oca, M., Carnero, A., Ortego, F. and Casteñera, P. (2007) Population structure of the banana weevil, an introduced pest in the Canary Islands, studied by RAPD analysis. Bulletin of Entomological Research 97, 585590.
Mantel, N. (1967) The detection of disease clustering and a generalized regression approach. Cancer Research 27, 209220.
Martins, W. F. S., Ayres, C. F. J. and Lucena, W. A. (2007) Genetic diversity of Brazilian natural populations of Anthonomus grandis Boheman (Coleoptera, Curculionidae), the major cotton pest in the New World. Genetics and Molecular Research 6, 2332.
McDermott, J. M. and McDonald, B. A. (1993) Gene flow in plant pathosystems. Annual Review of Phytopathology 31, 353373.
McMichael, M. and Prowell, D. P. (1999) Differences in amplified fragment-length polymorphisms in fall armyworm (Lepidoptera: Noctuidae) host strains. Annals of the Entomological Society of America 92, 175181.
Nahrung, H. F. and Allen, G. R. (2003) Geographical variation, population structure and gene flow between populations of Chrysophtharta agricola (Coleoptera: Chrysomelidae), a pest of Australian eucalypt plantations. Bulletin of Entomological Research 93,137144. doi: 10.1079/BER2003224.
Nei, M. (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences USA 70, 33213323.
Nei, M. (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89, 583590.
Nevo, E. (1978) Genetic variation in natural populations: patterns and theory. Theoretical Population Biology 13, 121177.
Ochieng, V. O. (2001) Genetic diversity in banana weevil Cosmopolites sordidus populations in banana growing regions of the world. PhD thesis, University of Nairobi, Kenya.
Ochieng, V. O., Osir, E. O. and Mulaa, F. J. (2002) Genetic biodiversity in banana weevil Cosmopolites sordidus populations in banana growing regions of the world. In Proceedings of the 7th Biochemical Society of Kenya Annual Scientific Conference, Biochemical Society of Kenya, Nairobi.
Ostmark, H. E. (1974) Economic insect pests of bananas. Annual Review of Entomology 19, 161176.
Padmanaban, B., Sundararaju, P., Velayudhan, K. C. and Sathiamoorthy, S. (2001) Evaluation of Musa Germplasm against banana weevil borers. InfoMusa 10(1), 2628.
Peakall, R. O. D. and Smouse, P. E. (2006) genalex 6: Genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6, 288295.
Pena, J. E, Duncan, R. and Martin, R. (1993) Biological control of Cosmopolites sordidus in Florida, pp. 124139. In Biological and Integrated Control of Highland Banana and Plantain Pests and Diseases: Proceedings of a Research Coordination Meeting (edited by Gold, C. S. and Gemmill, B.). IITA, Cotonou, Benin.
Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S. and Rafalski, A. (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding 2, 225238. doi:10.1007/BF00564200.
Prevost, A. and Wilkinson, M. J. (1999) A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics 98, 107112. doi:10.1007/s001220051046.
Ranjan, S. K., Basu Mallick, C., Saha, D., Vidyarthi, A. S. and Ramani, R. (2011) Genetic variation among species, races, forms and inbred lines of lac insects belonging to the genus Kerria (Homoptera, Tachardiidae). Genetics and Molecular Biology 34, 511519.
Reineke, A., Karlovsky, P. and Zebitz, C. P. W. (1999) Amplified fragment length polymorphism analysis of different geographic populations of gypsy moth, Lymantria dispar (Lepidoptera: Lymantriidae). Bulletin of Entomological Research 89, 7988.
Reyes, A. and Ochando, M. D. (1998) Use of molecular markers for detecting the geographical origin of Ceratitis capitata (Diptera: Tephritidae) populations. Annals of the Entomological Society of America 91, 222227.
Roush, R. T. and Daly, J. C. (1990) The role of population genetics in resistance research and management, pp. 97152. In Pesticide Resistance in Arthropods (edited by Roush, R. T. and Tabashnik, B. E.). Chapman & Hall Inc. New York.
Rukazambuga, N. D. T. M., Gold, C. S. and Gowen, S. R. (1998) Yield loss in East African highland banana (Musa spp., AAA-EA group) caused by the banana weevil, Cosmopolites sordidus Germar. Crop Protection 17, 581589.
Sidorenko, A. P. and Berezovska, O. P. (2002) Genetic structure of populations of the Colorado potato beetle Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Russian Journal of Genetics 38, 12561261. doi:10.1023/A:1021194927020.
Suinaga, F., Casali, V. W. D., Picanço, M. and Foster, J. E. (2004) Genetic divergence among tomato leafminer populations based on AFLP analysis. Pesquisa Agropecuária Brasileira 39, 645651.
Timm, A. E., Geertsema, H. and Warnich, L. (2008) Population genetic structure of Grapholita molesta (Lepidoptera: Tortricidae) in South Africa. Annals of the Entomological Society of America 101, 197203.
Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Friters, A., Pot, J., Paleman, J., Kuiper, M. and Zabeau, M. (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23, 44074414.
Welsh, J. and McClelland, M. (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research 18, 72137218.
Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A. and Tingey, S. V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 65316535.
Yeh, F. C., Rong-cai, Y. and Boyle, T. (1999) POPGENE version 1.31. University of Alberta and Centre for International Forestry Research, Edmonton, Alberta, Canada. Available at: https://sites.ualberta.ca/~fyeh/popgene.pdf.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

International Journal of Tropical Insect Science
  • ISSN: 1742-7584
  • EISSN: 1742-7592
  • URL: /core/journals/international-journal-of-tropical-insect-science
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed