Skip to main content

In vitro digestibility of the testa in tannin-free field beans (Vicia faba L.)

  • D. A. Bond (a1)

The seed-coat of all white-flowered varieties of Vicia faba and segregants tested was free of tannin. In two field trials, the whole grain of white-flowered varieties wassignificantly higher in in vitro digestibility of the dry matter, on average 4·7% higher, than coloured-flowered varieties of similar seed size. In subsequent trials where the testa and cotyledon were examined separately, this difference was shown to be largely due to a mean D value (digestibility of the organic matter) of the testa in tannin-free varieties of 56·4% compared with 17·2% in tannin-containing varieties. Seed size was positively correlated with D value but not because of differing proportions of testa.

Differences between varieties in acid-pepsin solubility and protein content of the testa were much smaller and not associated with presence of tannin. The testa colour of some tannin-free F2 plants was a darker shade of grey than either parental population. An inbred line completely lacking in anthocyanin in flowers and stem but with the normal black wing spot had tannin in its testa.

Grains of peas from the coloured-flowered variety, Minerva, reacted to a tannin test wheieas those from white-flowered varieties, Greenshaft and Flavanda, did not.

The case for breeding further tannin-free varieties of Vicia faba is discussed.

Hide All
Bate-Smith, E. C. & Lerner, N. H. (1954). Leucoanthocyanins. Biochemical Journal 58, 126.
Chang, S. I. & Fuller, H. L. (1964). Effect of tannin content of grain sorghums on their feeding value for growing chicks. Poultry Science 43, 30–6.
Dickinson, D., Knight, M. & Rees, D. I. (1957). Varieties of broad beans suitable for canning. Chemistry and Industry 16, 1503.
Donefer, E., Crampton, E. W. & Lloyd, L. E. (1966). The prediction of digestible energy intake potential (NV1) of forages using a simple in vitro technique. Proceedings of the Xth International Grassland Congress, pp. 442–5.
Erith, A. G. (1930). The inheritance of colour, size, form of seeds and of flower colour in Vicia faba L. Genetica 13, 477510.
Harris, H. B. (1969). Bird resistance in sorghum. Proceedings of the 24th Annual Corn and Sorghum Research Conference (Chicago), pp. 113–22.
Helle, J. -L. (1973). La Féverole. Memoire de fin d'Études, Institut National Agronomique, 12 Avenue George V, 75008, Paris.
Kendall, W. A. (1966). Factors affecting foams with forage legumes. Crop Science 6, 487–92.
Picard, J. (1963). La coloration des téguments du grain chez la féverole (Vicia faba L.). Étude de l'heredité des differentes colorations. Annales de l'Amélioration des Plantes 13, 97117.
Picard, J. (1975). Aperçu sur l'heredité du caractère absence de tanins dans les graines de féverole (Vicia faba L.). Annales de l'Amélioration des Plantes 26, 101–6.
Rogers, H. H. & Whitmore, E. T. (1966). A modified method for the in vitro determination of herbage digestibility in plant breeding studies. Journal of the British Grassland Society 21, 150–2.
Rowland, D. G. & Corner, J. (1962). Genetics of pigmentation in broad beans (Vicia fabaL.). Third Congress of Eucarpia, Paris, 229–34.
Schaffert, R. E., Lechtenberg, V. L., Oswalt, D. L., Axtell, J. D., Pickett, R. C. & Rhykerd, C. L. (1974). Effect of tannin on in vitro dry matter and protein disappearance in sorghum grain. Crop Science 14, 640–3.
Sirks, M. J. (1931 a). Beitrage zu einer genotypischen Analyse der Ackerbohne Vicia faba L. Genetica 13, 210631.
Sirks, M. J. (1931 b). Plasmatic influences upon the inheritance in Vicia faba. I. The elimination of a whole linkage group in the plasm of Vicia faba minor. Proceedings of Koninklijke Akadamie van Wetenschappen te Amsterdam, 34, 1057–62.
Sjodin, J. (1971). Induced morphological variation in Vicia faba L. Hereditas 67, 155–80.
Terry, R. A. & Tilley, J. M. A. (1964). The digestibility of the stems and leaves of perennial ryegrass, cocksfoot, timothy, tall fescue, lucerne and sainfoin as measured by an in vitro procedure. Journal of the British Grassland Society 19, 363–72.
Wilson, B. J. & McNab, J. M. (1972). The effect of autoclaving and methionine supplementation on the growth of chicks given diets containing field beans (Vicia faba L.). British Poultry Science 13, 6773.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

The Journal of Agricultural Science
  • ISSN: 0021-8596
  • EISSN: 1469-5146
  • URL: /core/journals/journal-of-agricultural-science
Please enter your name
Please enter a valid email address
Who would you like to send this to? *


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed