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Variation in the ovine keratin-associated protein 15-1 gene affects wool yield

Published online by Cambridge University Press:  28 November 2018

W. Li ,
H. Gong ,
H. Zhou Open the ORCID record for H. Zhou [Opens in a new window] ,
J. Wang ,
X. Liu ,
S. Li ,
Y. Luo  and
J. G. H. Hickford
W. Li
Affiliation:
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China International Wool Research Institute, Gansu Agricultural University, Lanzhou 730070, China
H. Gong
Affiliation:
International Wool Research Institute, Gansu Agricultural University, Lanzhou 730070, China Gene-marker Laboratory, Faculty of Agricultural and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
H. Zhou
Affiliation:
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China International Wool Research Institute, Gansu Agricultural University, Lanzhou 730070, China Gene-marker Laboratory, Faculty of Agricultural and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
J. Wang*
Affiliation:
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China International Wool Research Institute, Gansu Agricultural University, Lanzhou 730070, China
X. Liu
Affiliation:
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China International Wool Research Institute, Gansu Agricultural University, Lanzhou 730070, China
S. Li
Affiliation:
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China International Wool Research Institute, Gansu Agricultural University, Lanzhou 730070, China
Y. Luo
Affiliation:
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China International Wool Research Institute, Gansu Agricultural University, Lanzhou 730070, China
J. G. H. Hickford*
Affiliation:
International Wool Research Institute, Gansu Agricultural University, Lanzhou 730070, China Gene-marker Laboratory, Faculty of Agricultural and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
*
Authors for correspondence: J. Wang, E-mail: wangjq@gsau.edu.cn J. G. Hickford, E-mail: Jonathan.Hickford@Lincoln.ac.nz
Authors for correspondence: J. Wang, E-mail: wangjq@gsau.edu.cn J. G. Hickford, E-mail: Jonathan.Hickford@Lincoln.ac.nz
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Abstract

Keratin-associated proteins (KAPs) are constituents of wool and hair fibres and are believed to play an important role in determining the characteristics of the fibres. In the current study, a polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) approach was used to screen for variation in the ovine KAP15-1 gene (KRTAP15-1). Four PCR-SSCP banding patterns, representing four different variants (named A to D), were detected. Four single nucleotide polymorphisms were found within the coding region and three of these were non-synonymous. The effect of this genetic variation on wool traits was investigated in 396 Merino × Southdown-cross sheep. Of the three variants found in these sheep (A, B and C), the presence of B was found to be associated with decreased wool yield, while C was associated with increased wool yield and decreased fibre diameter standard deviation. Sheep of genotype AC had a higher wool yield than those of genotype AA or AB.

Keywords

KAP15-1 gene (KRTAP15-1)Keratin-associated protein (KAP)sheepwool yield
Type
Animal Research Paper
Information
The Journal of Agricultural Science , Volume 156 , Issue 7 , September 2018 , pp. 922 - 928
Copyright
Copyright © Cambridge University Press 2018 

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