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Exogenous phospholipase A2 affects inflammatory gene expression in primary bovine mammary epithelial cells

  • Jacqueline P. Kurz (a1) (a2) (a3), Mark P. Richards (a4), Matthew Garcia (a1) and Zhongde Wang (a1)

This Research Communication addresses the hypothesis that exogenously administered phospholipase A2 (PLA2) affects the inflammatory responses of bovine mammary epithelial cells (bMEC) in vitro with the aim of providing preliminary justification of investigation into the uses of exogenously administered PLA2 to manage or treat bovine mastitis. Primary bMEC lines from 11 lactating Holstein dairy cows were established and the expression of 14 pro-inflammatory genes compared under unchallenged and lipopolysaccharide (LPS)-challenged conditions, with and without concurrent treatment with bovine pancreatic PLA2G1B, a secreted form of PLA2. No differences in the expression of these genes were noted between PLA2-treated and untreated bMEC under unchallenged conditions. Following LPS challenge, untreated bMEC exhibited significant downregulation of CXCL8, IL1B, CCL20, and CXCL1. In contrast, PLA2-treated bMEC exhibited significant downregulation of IL1B and CCL20 only. These findings indicate that exogenous PLA2 affects the expression of some pro-inflammatory factors in immune-stimulated bMEC, but does not influence the constitutive expression of these factors. Further investigation of the influence of exogenous PLA2 in the bovine mammary gland is justified.

Corresponding author
Author for correspondence: Zhongde Wang, Email:
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Hanasaki, K (2004) Mammalian phospholipase A2: phospholipase A2 receptor. Biological & Pharmaceutical Bulletin 27, 11651167.
Jiang, L, Sorensen, P, Rontved, C, Vels, L and Ingvartsen, K (2008) Gene expression profiling of liver from dairy cows treated intra-mammary with lipopolysaccharide. BMC Genomics 9, 443.
Lahouassa, H, Moussay, E, Rainard, P and Riollet, C (2007) Differential cytokine and chemokine responses of bovine mammary epithelial cells to Staphylococcus aureus and Escherichia coli. Cytokine 38, 1221.
Lourenço, AG, Komesu, MC, Duarte, G, Del Ciampo, LA, Mussi-Pinhata, MM and Yamamoto, AY (2017) High levels of chemokine C–C motif ligand 20 in human milk and its production by oral keratinocytes. Breastfeeding Medicine 12, 116121.
Pareek, R, Wellnitz, O, Van Dorp, R, Burton, J and Kerr, D (2005) Immunorelevant gene expression in LPS-challenged bovine mammary epithelial cells. Journal of Applied Genetics 46, 171177.
Persson Waller, K, Colditz, IG, Lun, S and Östensson, K (2003) Cytokines in mammary lymph and milk during endotoxin-induced bovine mastitis. Research in Veterinary Science 74, 3136.
Rinaldi, M, Li, RW, Bannerman, DD, Daniels, KM, Evock-Clover, C, Silva, MVB, Paape, MJ, Van Ryssen, B, Burvenich, C and Capuco, AV (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis. Functional and Integrative Genomics 10, 2138.
Seroussi, E, Klompus, S, Silanikove, M, Krifucks, O, Shapiro, F, Gertler, A and Leitner, G (2013) Nonbactericidal secreted phospholipase A2s are potential anti-inflammatory factors in the mammary gland. Immunogenetics 65, 861871.
Wellnitz, O, Reith, P, Haas, SC and Meyer, HHD (2006) Immune relevant gene expression of mammary epithelial cells and their influence on leukocyte chemotaxis in response to different mastitis pathogens. Veterinarni Medicina 51, 125132.
Yedgar, S, Lichtenberg, D and Schnitzer, E (2000) Inhibition of phospholipase A2 as a therapeutic target. Biochimica et Biophysica Acta (BBA) – Molecular and Cell Biology of Lipids 1488, 182187.
Zhang, K, Zhang, D and Jiao, X (2013) full-text. European Review for Medical and Pharmacological Sciences 17, 32793284.
Zheng, J, Watson, AD and Kerr, DE (2006) Genome-wide expression analysis of lipopolysaccharide-induced mastitis in a mouse model. Infection and Immunity 74, 19071915.
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Journal of Dairy Research
  • ISSN: 0022-0299
  • EISSN: 1469-7629
  • URL: /core/journals/journal-of-dairy-research
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